Abstract:
:A method is described for the study of lymphokine-mediated cellular interactions using triple wells, which permits co-culture of cell subpopulations without direct physical contact. The triple wells are constructed by slitting the walls to half height between three adjacent wells of a 96-well microtiter plate. The cells under study are positioned in the outer two wells, whereas the middle well serves to separate the cells. The half slits permit the wells to be treated independently before filling the triple well with the culture medium and prevents cell leakage thereafter. The feasibility of the method was established by studying the interaction of isolated CD4+ and CD8+ T cell subsets during T cell proliferation induced by immobilized anti-CD3 and anti-CD28 monoclonal antibodies.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Karawajew L,Micheel B,Jung G,Wolf H,Behrsing Odoi
10.1016/0022-1759(94)90279-8subject
Has Abstractpub_date
1994-07-12 00:00:00pages
27-31issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(94)90279-8journal_volume
173pub_type
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