Abstract:
:We describe a rapid in vitro assay for the evaluation of in vivo properties of conjugate vaccines. Using human and murine monoclonal antibodies (mAb) specific for lipopolysaccharides (LPS), isolated from Pseudomonas aeruginosa, we determined in a competitive binding assay the amount of LPS or conjugate vaccine which was required to inhibit the antibody binding to LPS by 50% (I50 values). Furthermore, utilizing a murine burn wound sepsis model, we determined the potential of the same conjugates to induce protection in vivo against infection with the corresponding bacteria. Protective mAb have approximately 100-fold lower I50 values for preparations which are highly effective in inducing protection than for preparations which are ineffective. Furthermore, in the case of potent preparations it was noted that protective mAb exhibit similar I50 values for the conjugates and for the corresponding LPS. These results suggest that the fast and easily interpretable in vitro assay described may significantly facilitate the development and optimization of vaccines.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Bruderer U,Fürer E,Cryz SJ Jr,Lang ABdoi
10.1016/0022-1759(90)90367-5subject
Has Abstractpub_date
1990-10-19 00:00:00pages
263-8issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(90)90367-5journal_volume
133pub_type
杂志文章abstract::The aim of the study was to develop a novel system permitting automated analysis of multicolor immunofluorescence-staining of cells in solid tissues which would be comparable to the analytical capacity of flow cytometry. In the user friendly automated data acquisition and image processing system which was established,...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2000-04-03 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(98)00190-2
更新日期:1999-01-01 00:00:00
abstract::Many antigens are shared between different types of human leukocytes. In an effort to obtain new lineage-specific monoclonal antibodies, particularly antibodies to dendritic cells, we attempted to tolerize newborn mice to one type of leukocyte and then immunize the adults with another. We found that T cells, either un...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2015.08.006
更新日期:2015-11-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1994-01-03 00:00:00
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pub_type: 杂志文章
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更新日期:1989-09-29 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00115-q
更新日期:1995-09-25 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(81)90135-6
更新日期:1981-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00210-3
更新日期:1995-12-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(76)90211-8
更新日期:1976-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90158-8
更新日期:1983-07-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2005.10.012
更新日期:2006-01-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00241-3
更新日期:1996-02-05 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:1992-09-18 00:00:00
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journal_title:Journal of immunological methods
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更新日期:1985-11-07 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1983-02-25 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(80)90058-7
更新日期:1980-01-01 00:00:00
abstract::A simple semi-automated method for the assay of large numbers of replicate microcultures for the presence of antibody-forming cell clones is described. The supernatant medium is removed from microcultures by a single sharp flick on inverting the tray. The cultured cells are mixed with 0.05 ml of a plaque-revealing mix...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(82)90346-5
更新日期:1982-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1991-07-05 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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