CTL lysis: there is a hyperbolic relation of killing rate to exocytosable granzyme A for highly cytotoxic murine cytotoxic T lymphocytes.

abstract：:The majority of immunological processes are mediated by cell-to-cell contact or receptor-ligand interactions that transmit intracellular signals and affect the regulation of transcription in the nucleus. As a consequence, precursor cells develop into their respective lineages and cells differentiate further during an ...

journal_title：Journal of immunological methods

authors： Ali M,Markham AF,Isaacs JD

更新日期：2001-04-01 00:00:00

abstract：:We have developed and characterised a variant of the human T-cell tumour, MOLT-4F, which is resistant to both 8-azaguanine and 6-thioguanine. This new cell line, M4HS2, is suitable for the efficient production of lymphokine-secreting human T-cell hybrids and is available for distribution. ...

journal_title：Journal of immunological methods

authors： Gallagher G,Christie JF,Boyd E,Stimson WH

更新日期：1986-06-10 00:00:00

abstract：:A practical mixed hemadsorption assay (MHA) was developed for the detection of cell surface binding anti-tumor antigen antibodies (anti-TA Ab) in the human sera. The assay was compared to enzyme-linked immunosorbent assay (ELISA) and dot ELISA in the detection of the antibodies developed against GA733-2 antigen. SW111...

journal_title：Journal of immunological methods

authors： Akis N,Andl C,Zhou D

更新日期：2002-03-01 00:00:00

abstract：:We report the development of an expression system for the production of soluble, calmodulin (CaM)-tagged proteins in Drosophila Schneider S2 cells and the subsequent use of these proteins for the selection of phage displayed antibodies. The CaM-tag permitted the purification of recombinant protein to >90% purity in a ...

journal_title：Journal of immunological methods

authors： Jennings NS,Smethurst PA,Knight CG,O'Connor MN,Joutsi-Korhonen L,Stafford P,Stephens J,Garner SF,Harmer IJ,Farndale RW,Watkins NA,Ouwehand WH

更新日期：2006-10-20 00:00:00

abstract：:Autoimmune (Hashimoto's) thyroiditis is a chronic inflammatory disease which affects >3% of the population and shows an increasing prevalence with increasing age. Anti-thyroid autoantibodies, particularly against thyroperoxidase (also known as thyroid peroxidase or TPO), occur commonly in humans with autoimmune thyroi...

journal_title：Journal of immunological methods

authors： Hayward SL,Suzuki K,Elliott JF

更新日期：2007-06-30 00:00:00

abstract：:The common marmoset is a small New World Primate that has been used as an immunological model for a number of human diseases. Dendritic cells (DC) have not been extensively characterised in this species and in particular protocols to derive DC from living donors without the need for animal sacrifice are presently lack...

journal_title：Journal of immunological methods

authors： Prasad S,Kireta S,Leedham E,Russ GR,Coates PT

更新日期：2010-01-31 00:00:00

abstract：:A sensitive direct assay has been developed to quantitate antigen specific helper factors (ThF). The assay depends on the ability of ThF to block the binding of free antibody to immobilized antigen. It has been used to follow the course of purification of ThF produced in short-term cultures of in vitro primed helper c...

journal_title：Journal of immunological methods

authors： Kilburn DG,Crane S,Altin M

更新日期：1984-02-24 00:00:00

abstract：:Studies describing the induction of apoptosis for CD4 mAbs do not delineate between epitope-dependent and Fc-driven epitope cross-linking induced cell death. Keliximab and clenoliximab are two CD4 mAbs that differ only in their heavy chain isotypes, being an IgG1 and a modified IgG4, respectively. These antibodies sup...

journal_title：Journal of immunological methods

authors： Fishman-Lobell J,Tsui P,Reddy M,DiPrinzio R,Eichman C,Sweet RW,Truneh A

更新日期：2001-11-01 00:00:00

abstract：:We describe here a spot test to detect antibodies to non-erythroid cell surface antigens, using ethidium bromide staining to visualize areas of target cell lysis. This assay allows rapid screening of large numbers of samples of culture supernatants, antisera, or ascitic fluids. Antibody titers determined by this spot ...

journal_title：Journal of immunological methods

authors： Cohn ML,Golding H,Rittenberg MB

更新日期：1982-01-01 00:00:00

abstract：:The purpose of this study was to develop a flow cytometric method for the detection of adenosine deaminase (ADA) in a single cell suspension of mononuclear cells. Anti-human ADA antibody was purified by affinity chromatography on a column of Sepharose 4B to which calf ADA was covalently linked. This antibody was used ...

journal_title：Journal of immunological methods

authors： SenGupta S,Petsche D,Gelfand EW,Chechik BE

更新日期：1985-06-25 00:00:00

abstract：:Surface plasmon resonance is a valuable optical phenomenon for monitoring biomolecular interactions in real time. In this project anti-mouse-Fc was coupled to the carboxymethyl dextran coating on the surface of a CM5 sensor chip (BIAcore) using amine coupling. Monoclonal antibodies (MAbs) to Luteinizing Hormone (LH) w...

journal_title：Journal of immunological methods

authors： Pearson JE,Kane JW,Petraki-Kallioti I,Gill A,Vadgama P

更新日期：1998-12-01 00:00:00

abstract：BACKGROUND:The role of CD4(+) regulatory T cells (Tregs) and their subsets during HIV infection is controversial. Cryopreserved peripheral blood mononuclear cells (PBMC) are an important source for assessing number and function of Tregs. However, it is unknown if PBMC isolation and cryopreservation affect the expressio...

journal_title：Journal of immunological methods

authors： Zhang W,Nilles TL,Johnson JR,Margolick JB

更新日期：2016-04-01 00:00:00

abstract：:We report a method for the precise mapping of linear epitopes by presenting a peptide library on the surface of Escherichia coli cells. A random library of gene fragments derived from the classical swine fever virus (CSFV) envelope protein E(rns) was generated by DNAse I cleavage and cloned into a specially designed b...

journal_title：Journal of immunological methods

authors： Christmann A,Wentzel A,Meyer C,Meyers G,Kolmar H

更新日期：2001-11-01 00:00:00

abstract：:Dot-immunobinding assay for anti-calmodulin (CaM), and immunoblot assay for CaM have become feasible by proper choice of a fixative, i.e., formaldehyde vapor. The protocol for these simple and convenient immunological procedures is presented. Dot-immunobinding assay could be accomplished in 3 h, and immunoblot assay i...

journal_title：Journal of immunological methods

authors： Natsukari N,Ohta H,Fujita M

更新日期：1989-12-20 00:00:00

abstract：:Elucidation of local immune response at the cervix is important for understanding and evaluating STD vaccine approaches currently being proposed. However, no well-validated method exists for the collection of cervical secretions for evaluation of cervical immune response. The purpose of this study was to determine the...

journal_title：Journal of immunological methods

authors： Hildesheim A,McShane LM,Schiffman M,Bratti MC,Rodriguez AC,Herrero R,Morera LA,Cardenas F,Saxon L,Bowman FP,Crowley-Nowick PA

更新日期：1999-05-27 00:00:00

abstract：:Use of fluorescently labelled multimers, particularly tetramers of peptide and MHC class I glycoprotein (pMHC-I) complexes, is essential for the analysis of CD8+ T cell immunity in basic research and clinical settings. A recently described combinatorial approach using pMHC-I multimers coupled to a unique combination o...

journal_title：Journal of immunological methods

authors： Cukalac T,Valkenburg SA,La Gruta NL,Turner SJ,Doherty PC,Kedzierska K

更新日期：2010-08-31 00:00:00

abstract：:An antibody detection ELISA was developed for diagnosis of visceral leishmaniasis. Antigens released by Leishmania donovani promastigotes into a protein-free medium were used. SDS-PAGE analysis has indicated that Ld-ESM contain several protein antigens. Titration and chequer-board analyses were performed to optimise t...

journal_title：Journal of immunological methods

authors： Rajasekariah GH,Ryan JR,Hillier SR,Yi LP,Stiteler JM,Cui L,Smithyman AM,Martin SK

更新日期：2001-06-01 00:00:00

abstract：:Fab's with hinges based on the human gamma1 sequence containing 1, 2, or 4 cysteines have been produced by high level Escherichia coli periplasmic secretion, and coupled in vitro by reduction/oxidation to form F(ab')2. We find that the F(ab')2 made with hinges containing 2 or 4 cysteines have a high level (approximate...

journal_title：Journal of immunological methods

authors： Humphreys DP,Vetterlein OM,Chapman AP,King DJ,Antoniw P,Suitters AJ,Reeks DG,Parton TA,King LM,Smith BJ,Lang V,Stephens PE

更新日期：1998-08-01 00:00:00

abstract：:In this study the exposure period of the lymphocyte-myeloma cell mixture to the fusogen was evaluated for its influence upon the yield of total hybridoma colonies and those which secreted monoclonal antibodies. Sp2/0 and FOX-NY myeloma cells were fused for varying periods with murine splenic lymphocytes immunized with...

journal_title：Journal of immunological methods

authors： Lane RD

更新日期：1985-08-02 00:00:00

abstract：:The neutrophil respiratory burst is essential for the host's ability to kill ingested microorganisms. Several flow cytometric assays have recently been developed to measure this process. These assays are largely unvalidated. In this study a whole blood flow cytometry assay using dihydrorhodamine 123 (DHR) as a substra...

journal_title：Journal of immunological methods

authors： Richardson MP,Ayliffe MJ,Helbert M,Davies EG

更新日期：1998-10-01 00:00:00

abstract：:The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measur...

journal_title：Journal of immunological methods

authors： Glier H,Heijnen I,Hauwel M,Dirks J,Quarroz S,Lehmann T,Rovo A,Arn K,Matthes T,Hogan C,Keller P,Dudkiewicz E,Stüssi G,Fernandez P,Swiss Cytometry Society.

更新日期：2019-12-01 00:00:00

abstract：:In order to isolate monoclonal antibodies (mAbs) that bind to tumor-associated antigens (Ags) we developed the following strategy. Using the phage-display Ab library we isolated a large number of mAbs that bind to the surface of human tumor cells. The mAbs were individually screened by immunostaining, and clones that ...

journal_title：Journal of immunological methods

authors： Kurosawa G,Sumitomo M,Akahori Y,Matsuda K,Muramatsu C,Takasaki A,Iba Y,Eguchi K,Tanaka M,Suzuki K,Morita M,Sato N,Sugiura M,Sugioka A,Hayashi N,Kurosawa Y

更新日期：2009-12-31 00:00:00

abstract：:We describe the preparation of a new rat monoclonal antibody (CRC1) to the N-terminal sequence of the 43 kDa subunit of human ovarian inhibin, and its use together with other anti-peptide monoclonal antibodies, in two-site immunoassays for the detection of inhibin-related material in biological fluids. The Fab fractio...

journal_title：Journal of immunological methods

authors： Groome N,O'Brien M

更新日期：1993-10-15 00:00:00

abstract：:The length of the heavy chain complementarity-determining region two (HCDR2) of the unmutated anti-p-azophenylarsonate (Ars) monoclonal antibody (36-65 mAb) was extended by three residues in order to test whether this insertion can provide additional contacts between the Ab and the antigen. Two libraries were generate...

journal_title：Journal of immunological methods

authors： Parhami-Seren B,Viswanathan M,Margolies MN

更新日期：2002-01-01 00:00:00

abstract：:It is known that membranoproliferative glomerulonephritis (MPGN), hypocomplementaemia and C3 nephritic factor (C3NeF) are closely related to each other, and the presence or absence of C3NeF in the serum is important for evaluating the nature of MPGN. However, some difficulties have been encountered in detecting this f...

journal_title：Journal of immunological methods

authors： Ohi H,Watanabe S,Fujita T,Seki M,Hatano M

更新日期：1990-07-20 00:00:00

abstract：:There are several double immunolabelling methods but each has its drawbacks. More often than not, antibodies with the required specificities are available in only one species and their use normally produces false labels due to cross-reactivity. We describe a new and reliable technique for staining with primary antibod...

journal_title：Journal of immunological methods

authors： Eichmüller S,Stevenson PA,Paus R

更新日期：1996-04-19 00:00:00

abstract：:A two-stage culture method is described for the induction of a specific antibody response to sheep red cells (SRC) in microcultures at limiting dilutions of human peripheral blood lymphocytes (PBL). PBL from normal donors were cultured for 4 days with antigen and EBV using well defined conditions. The cells were then ...

journal_title：Journal of immunological methods

authors： Luzzati AL,Giacomini E,Frugoni P

更新日期：1988-04-22 00:00:00

abstract：BACKGROUND:In light of the HIV pandemic, significant strides have been made in improving treatment options for patients. Technologies to monitor the progress of a patient on such treatment have therefore also been scaled up. Immune activation as measured by CD38 mean fluorescence intensity (MFI) on CD8 T cells has been...

journal_title：Journal of immunological methods

authors： Moodley K,Coetzee LM,Glencross DK

更新日期：2012-04-30 00:00:00

abstract：:Peritoneal eosinophilia frequently occurs in patients undergoing peritoneal dialysis. We have devised a method for isolating large numbers of these peritoneal eosinophils from pediatric patients on continuous peritoneal dialysis. Patients were selected on the basis of previous high peritoneal eosinophil counts and had...

journal_title：Journal of immunological methods

authors： Roberts RL,Ank BJ,Salusky IB,Stiehm ER

更新日期：1990-02-09 00:00:00

abstract：:Polymorphisms of immune defence genes may act as disease modifiers and are studied by many researchers. A conclusive analysis of the impact of genetic variations typically requires a large number of sample specimens, and in retrospective studies this may include samples of reduced quality, e.g. formalin-fixed paraffin...

journal_title：Journal of immunological methods

authors： Mølle I,Melsvik D,Østergaard M

更新日期：2007-07-31 00:00:00