Abstract:
:The neutrophil respiratory burst is essential for the host's ability to kill ingested microorganisms. Several flow cytometric assays have recently been developed to measure this process. These assays are largely unvalidated. In this study a whole blood flow cytometry assay using dihydrorhodamine 123 (DHR) as a substrate was compared with the quantitative nitrobluetetrazolium (NBT) test, an accepted measure of the earliest events in the respiratory burst. Because whole blood is used, the new assay is quicker and simpler than existing flow cytometry assays. Specimens as small as 0.1 ml can be used which makes the assay ideal for use in neonates and young children. There was a high degree of correlation between the DHR assay and the quantitative NBT test (r(s) = 0.76, P < 0.01). It is concluded that the whole blood DHR assay is an accurate and sensitive measure of the respiratory burst.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Richardson MP,Ayliffe MJ,Helbert M,Davies EGdoi
10.1016/s0022-1759(98)00136-7subject
Has Abstractpub_date
1998-10-01 00:00:00pages
187-93issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(98)00136-7journal_volume
219pub_type
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