Specific quantitation of secretory immunoglobulin A with enzyme immunoassay using activated thiol--Sepharose for separation method.

abstract：:In this study we have employed surface plasmon resonance to examine the kinetic binding constants of a new class of soluble cytokine receptor complexes. The solution assembly of both homomeric and heteromeric interleukin-2 receptor ectodomain complexes has been achieved using coiled-coil molecular recognition. These c...

journal_title：Journal of immunological methods

authors： Wu Z,Johnson KW,Ciardelli TL

更新日期：1995-06-14 00:00:00

abstract：:Immune analysis of polypeptides separated by SDS-PAGE provides a technique useful in the characterisation of complex mixtures. We describe a simple procedure involving immune analysis of fractionated PAGE gels. Polypeptides of purified herpesvirus simplex, HSV 'excreted antigen' and cell culture 'control' preparations...

journal_title：Journal of immunological methods

authors： Cleator GM,Klapper PE,Cropper LM,Coupes D,Longson M

更新日期：1984-03-16 00:00:00

abstract：:The length distributions of the third complementarity determining region (CDR3) of the T cell receptor (TCR) beta chain were determined quantitatively from peripheral blood lymphocytes of healthy donors. RT-PCR products of 26 V beta families and subfamilies were analyzed by an A.L.F. DNA sequencer and Fragment Manager...

journal_title：Journal of immunological methods

authors： Liu D,Callahan JP,Dau PC

更新日期：1995-11-16 00:00:00

abstract：:A high-cloning efficiency, filler cell-free limit-dilution culture system for the growth and differentiation of single cytotoxic T lymphocyte precursors (CTLp) was tested for its ability to maintain the lytic specificity of the resultant clones of cytotoxic T lymphocytes (CTL). The system used non-specific stimulation...

journal_title：Journal of immunological methods

authors： Rogers LA,Zlotnik A,Lee F,Shortman K

更新日期：1991-10-25 00:00:00

abstract：:The use of colloidal gold and colloidal gold followed by silver enhancement as marker for dot or blot immune overlays is described. Colloidal gold probes concentrating at the sites of immune reaction gradually develop a pinkish colour during incubation that can be seen with the naked eye. With a physical developer, ve...

journal_title：Journal of immunological methods

authors： Moeremans M,Daneels G,Van Dijck A,Langanger G,De Mey J

更新日期：1984-11-30 00:00:00

abstract：:An improved fixation method for antigen detection in lymphoblastoid cells is described. Herpesvirus papio nuclear antigen (HUPNA) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (ACIF). Antibody to HUPNA was detected in many human sera containing antibodies to Epstein-...

journal_title：Journal of immunological methods

authors： Schmitz H

更新日期：1981-01-01 00:00:00

abstract：:A monoclonal antibody to human IgG was tested with myeloma proteins of the four IgG subclasses. When tested by immunofluorometric assay, enzyme-linked immunosorbent assay, hemagglutination and hemagglutination inhibition assays, the antibody reacted with IgG3 but not with the other three IgG subclasses. When tested by...

journal_title：Journal of immunological methods

authors： Molinaro GA,Eby WC,Reimer C

更新日期：1987-02-11 00:00:00

abstract：:To increase the selection efficiency and productivity of stable clones expressing recombinant antibody-IL-2 fusion protein, a dicistronic expression vector containing the anti-erbB2 scFv-Fc-IL-2 fusion gene followed by a weakened neo gene, was constructed to allow for the concurrent translation of the recombinant prot...

journal_title：Journal of immunological methods

authors： Chen L,Xie Z,Teng Y,Wang M,Shi M,Qian L,Hu M,Feng J,Yang X,Shen B,Guo N

更新日期：2004-12-01 00:00:00

abstract：:We describe here a specific and sensitive assay for biologically active bovine type-I interferon (IFN) in an Mx/CAT reporter gene assay. The assay is based on Madin-Darby Bovine Kidney cells transfected with a plasmid, containing a human MxA promoter driving a chloramphenicol acetyltransferase (CAT) cDNA. CAT expressi...

journal_title：Journal of immunological methods

authors： Fray MD,Mann GE,Charleston B

更新日期：2001-03-01 00:00:00

abstract：:The microenvironment of the thymus consists of functionally discrete niches composed of distinct stromal cell subsets. Clinically relevant changes affecting T-cell differentiation occur within these niches with age and injury caused by irradiation and chemotherapy treatments. The study of thymic stromal cells has been...

journal_title：Journal of immunological methods

authors： Gray DH,Fletcher AL,Hammett M,Seach N,Ueno T,Young LF,Barbuto J,Boyd RL,Chidgey AP

更新日期：2008-01-01 00:00:00

abstract：:Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera ven...

journal_title：Journal of immunological methods

authors： Asseman C,Pancré V,Delanoye A,Capron A,Auriault C

更新日期：1994-07-12 00:00:00

abstract：:An indirect rosette assay which utilizes erythrocytes coupled with purified antiimmunoglobulin (Ig) antibodies was modified into a microassay for detecting HLA allo-and xenoantibodies. The test, which is performed in microtiter plates, is specific, reproducible and can handle large numbers of samples. As an inhibition...

journal_title：Journal of immunological methods

authors： Indiveri F,Wilson BS,Pellegrino MA,Ferrone S

更新日期：1979-01-01 00:00:00

abstract：:Progress in elucidating the pathogenesis of Helicobacter pylori gastric infection and in developing an H. pylori vaccine will be aided by an animal model in which H. pylori can be reliably detected. To validate the use of the mouse model of H. pylori infection, we determined the susceptibility of three inbred strains ...

journal_title：Journal of immunological methods

authors： Smythies LE,Chen JA,Lindsey JR,Ghiara P,Smith PD,Waites KB

更新日期：2000-08-28 00:00:00

abstract：:A serum-free lymphocyte culture medium is described in which serum is replaced by bovine serum albumin, transferrin, insulin, ethanolamine and a mixture of saturated and unsaturated fatty acids (linoleic acid, oleic acid and palmitic acid). In this serum-free medium proliferative and cytotoxic responses induced in mix...

journal_title：Journal of immunological methods

authors： Yssel H,De Vries JE,Koken M,Van Blitterswijk W,Spits H

更新日期：1984-08-03 00:00:00

abstract：:Microchannel plate detectors have been used in many astronomical X-ray telescopes. Recently we have begun to use similar detectors to image electron emission from radiolabelled biological assays. Here we show how a microchannel plate (MCP) detector can be used to image tritium uptake in T lymphocyte proliferation assa...

journal_title：Journal of immunological methods

authors： Lees JE,Hales JM

更新日期：2001-01-01 00:00:00

abstract：:Interferon gamma (IFNγ) is a cytokine involved in many anti-viral and immunoregulatory processes. One of the major mechanisms through which IFNγ exerts these effects is by inducing expression of indoleamine 2,3 dioxygenase-1 (IDO1), an enzyme that catalyses the first, rate-limiting step of the kynurenine pathway. In t...

journal_title：Journal of immunological methods

authors： Jusof FF,Khaw LT,Ball HJ,Hunt NH

更新日期：2013-08-30 00:00:00

abstract：:The functional integrity of classical complement pathway components was determined by an immunofluorescence (IFL) assay based on the capacity of cytoskeletal intermediate filaments (IMF) to bind C1q and to activate the complement pathway. The assay uses IMF-rich capillary endothelium of human term placentae as complem...

journal_title：Journal of immunological methods

authors： Linder E,Rhen M,Meri S

更新日期：1981-01-01 00:00:00

abstract：:We have developed a sensitive and flexible method for the qualitative evaluation of IgG-Fc receptor interactions in cell suspensions. The assay is based on the flow cytometric quantitation of antibody-coated erythrocyte (EA) rosetting using fluorescein-labelled indicator erythrocytes (E). The number of IgG molecules o...

journal_title：Journal of immunological methods

authors： Tuijnman WB,Van de Winkel JG,Capel PJ

更新日期：1990-03-09 00:00:00

abstract：:The level of CD4(+) T-lymphocytes represents a useful marker with which to monitor the progression of HIV infection. Sex and geographical differences in the reference values of lymphocyte subsets have been reported. We have compared two flow cytometric methods (MultiSET and SimulSET) for the quantification of lymphocy...

journal_title：Journal of immunological methods

authors： Urassa WK,Mbena EM,Swai AB,Gaines H,Mhalu FS,Biberfeld G

更新日期：2003-06-01 00:00:00

abstract：:Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associated serine proteas...

journal_title：Journal of immunological methods

authors： Selman L,Henriksen ML,Brandt J,Palarasah Y,Waters A,Beales PL,Holmskov U,Jørgensen TJ,Nielsen C,Skjodt K,Hansen S

更新日期：2012-01-31 00:00:00

abstract：:This study investigates the applicability of D-PAM, the inverso form of the Protein A Mimetic synthetic peptide affinity ligand (PAM) obtained from the screening of a multimeric combinatorial peptide library, in monoclonal IgG isolation from ascitic fluids and cellular supernatants. D-PAM affinity columns, prepared by...

journal_title：Journal of immunological methods

authors： D'Agostino B,Bellofiore P,De Martino T,Punzo C,Rivieccio V,Verdoliva A

更新日期：2008-04-20 00:00:00

abstract：:The U937 cell line has been shown to generate tumour necrosis factor alpha (TNF-alpha) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-alpha from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-trea...

journal_title：Journal of immunological methods

authors： Jiang WG,Puntis MC,Hallett MB

更新日期：1992-08-10 00:00:00

abstract：:Neutrophils are a critical component of the innate immune response to invading microbial pathogens. However, an excessive and/or prolonged neutrophil response can result in tissue injury that is thought to underlie the pathogenesis of various inflammatory diseases. The development of novel therapeutic strategies for i...

journal_title：Journal of immunological methods

authors： Zemans RL,Briones N,Young SK,Malcolm KC,Refaeli Y,Downey GP,Worthen GS

更新日期：2009-01-30 00:00:00

abstract：:Pathological prion protein (PrPSc) which is a conformational isoform of a host-encoded protein designated (PrPC) serves as a specific marker protein for the immunochemical diagnosis of transmissible spongiform encephalopathies (TSE). The generation of suitable antibodies to PrPSc therefore underlies the specificity an...

journal_title：Journal of immunological methods

authors： Groschup MH,Harmeyer S,Pfaff E

更新日期：1997-08-22 00:00:00

abstract：:We have developed and validated a process-specific immunoligand assay based on the Threshold system for the quantification of residual host cell proteins (HCPs) in a recombinant subunit vaccine candidate against the human respiratory syncytial virus (hRSV). The industrial process of this vaccine produced in Escherichi...

journal_title：Journal of immunological methods

authors： Dagouassat N,Haeuw JF,Robillard V,Damien F,Libon C,Corvaïa N,Lawny F,Nguyen TN,Bonnefoy JY,Beck A

更新日期：2001-05-01 00:00:00

abstract：:A sensitive, specific immunoassay for detection of hepatitis B surface antigen (HBsAg) is described. The assay combined enzyme-linked immunosorbent assay and solid-phase radioimmunoassay and is termed enzyme potentiated radioimmunoassay (EPRIA). HBsAg was quantitated by enzymatic conversion of L[14C]glutamic acid to 1...

journal_title：Journal of immunological methods

authors： Fields HA,Davis CL,Dreesman GR,Bradley DW,Maynard JE

更新日期：1981-01-01 00:00:00

abstract：:Structural point mutations in exon 1 at codons 52, 54 and 57 and a promotor polymorphism at -221 bp of the mannose-binding lectin (MBL) gene are associated with increased susceptibility to various infectious diseases. We developed a genotyping method based on the 5' nuclease (TaqMan) assay in combination with the use ...

journal_title：Journal of immunological methods

authors： Van Hoeyveld E,Houtmeyers F,Massonet C,Moens L,Van Ranst M,Blanckaert N,Bossuyt X

更新日期：2004-04-01 00:00:00

abstract：:Cephalexin was structurally modified by the attachment of a spacer at the carboxylic acid through which it was subsequently covalently attached to BSA. This method permitted the molecule to be attached without cleavage of the beta-lactam ring giving a conjugate distinct from previously described immunogenic preparatio...

journal_title：Journal of immunological methods

authors： Kachab EH,Wu WY,Chapman CB

更新日期：1992-02-14 00:00:00

abstract：:An anti-human T-lymphocyte antiserum has been further studied for specificity for T-cell subpopulations and application in tissue sections. Using a complement-dependent microcytotoxicity assay about 60% of normal peripheral blood T-cells were found to be sensitive to lysis, while 40% were resistant. When the T-cell su...

journal_title：Journal of immunological methods

authors： Braathen LR

更新日期：1980-01-01 00:00:00

abstract：:A new rapid staining and measuring method has been developed for the quantification of migrated cells in a microchemotaxis chamber. The migrated cells were, after staining, evaluated by a transmission densitometer. The method introduced here is more accurate and faster than those described previously. In addition the ...

journal_title：Journal of immunological methods

authors： Klomp JP,Te Velde AA,Figdor CG

更新日期：1989-03-10 00:00:00