Abstract：
:Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associated serine protease 1 (MASP-1) and/or MASP-3 in circulation. Mutation in the CL-11 gene was recently associated with the developmental syndrome 3MC. In the present study, we established and thoroughly validated a sandwich enzyme-linked immunosorbent assay (ELISA) based on two different monoclonal antibodies. The assay is highly sensitive, specific and shows excellent quantitative characteristics such as reproducibility, dilution linearity and recovery (97.7-104%). The working range is 0.15-34 ng/ml. The CL-11 concentration in two CL-11-deficient individuals affected by the 3MC syndrome was determined to be below 2.1 ng/ml. We measured the mean serum CL-11 concentration to 284 ng/ml in 100 Danish blood donors, with a 95% confidence interval of 269-299 ng/ml. There was no significant difference in the CL-11 concentration measured in matched serum and plasma samples. Storage of samples and repeated freezing and thawing to a certain extent did not influence the ELISA. This ELISA offers a convenient and reliable method for studying CL-11 levels in relation to a variety of human diseases and syndromes.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Selman L,Henriksen ML,Brandt J,Palarasah Y,Waters A,Beales PL,Holmskov U,Jørgensen TJ,Nielsen C,Skjodt K,Hansen Sdoi
10.1016/j.jim.2011.10.010subject
Has Abstractpub_date
2012-01-31 00:00:00pages
182-8issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(11)00291-2journal_volume
375pub_type
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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