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U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor alpha.

Abstract：

:The U937 cell line has been shown to generate tumour necrosis factor alpha (TNF-alpha) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-alpha from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM-CSF. The release of TNF-alpha from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil 'priming'. The biological activities in the cell supernatant were inhibited by TNF-alpha antiserum. Phagocytosis was required for TNF-alpha production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-alpha production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-alpha which can be generated conveniently and cheaply for experimental investigations.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Jiang WG,Puntis MC,Hallett MB

doi

10.1016/0022-1759(92)90141-f

keywords:

subject

Has Abstract

pub_date

1992-08-10 00:00:00

pages

201-7

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(92)90141-F

journal_volume

152

pub_type

杂志文章

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