Abstract:
:Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera venom hypersensitivity (HVH). We have developed a radioimmunoassay specific for ubiquitin, in order to determine the ubiquitin levels in human sera and plasma from patients with these pathologies. The working range of the assay was between 60 and 500 ng/ml, and the sensitivity was 8-10 ng/ml. The reproducibility, specificity and accuracy were determined under standard condition (PBS-0.3% BSA) and using different biological fluids (human serum, plasma and T lymphocyte supernatant). Using this assay, we found that the ubiquitin concentrations were higher in both schistosomiasis and HVH (up to 150-300 ng/ml) compared with sera and plasma from healthy donors where the ubiquitin levels did not exceed 50 ng/ml.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Asseman C,Pancré V,Delanoye A,Capron A,Auriault Cdoi
10.1016/0022-1759(94)90286-0subject
Has Abstractpub_date
1994-07-12 00:00:00pages
93-101issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(94)90286-0journal_volume
173pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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