当前位置： SCI文献检索 > JOURNAL OF IMMUNOLOGICAL METHODS期刊下所有文献 > The development of monoclonal human rabies virus-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure.

The development of monoclonal human rabies virus-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure.

Abstract：

:To provide a more defined and safer replacement for the human rabies immune globulin (HRIG) from pooled serum which is currently used for treatment of exposure to rabies virus we have developed a series of human rabies virus-specific monoclonal antibodies. Mouse-human heterohybrid myeloma cells producing rabies virus-specific human monoclonal antibodies were prepared using B cells obtained from volunteers recently-immunized with a commercial rabies virus vaccine (HDCV). Cell lines producing antibody which neutralized the Evelyn-Rokitnicki-Abelseth (ERA) rabies virus strain in vitro were cloned and the resulting monoclonal antibodies characterized for isotype, specificity against a variety of rabies virus isolates, and neutralization capacity. The ability of the monoclonal antibodies to neutralize a variety of rabies virus strains in vitro correlated with their binding specificity for these viruses in an enzyme-linked immunoadsorbant assay (ELISA). A number of these antibodies have proven suitable for the formulation of a prophylactic human monoclonal antibody-based reagent which would provide significant advantages to the HRIG in having defined, reproducible specificity, lessened possibility of contamination with viral pathogens, and consistent availability.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Champion JM,Kean RB,Rupprecht CE,Notkins AL,Koprowski H,Dietzschold B,Hooper DC

doi

10.1016/s0022-1759(99)00223-9

keywords:

subject

Has Abstract

pub_date

2000-02-21 00:00:00

pages

81-90

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(99)00223-9

journal_volume

235

pub_type

杂志文章

相关文献

JOURNAL OF IMMUNOLOGICAL METHODS文献大全
  • Purification of uteroglobin using monospecific antibodies coupled to divinylsulphone-activated agarose.

    abstract：:As a model for the isolation of a labile or trace protein, the purification of uteroglobin (UGL) by immunoaffinity chromatography is described. Antibody was isolated from sheep antiserum by immunoprecipitation, and coupled to divinylsulphone-activated agarose (Mini Leak). For the immunoabsorption stage rabbit uterine ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(89)90400-6

    authors： Dannhorn DR,Wirth B,Kirchner C

    更新日期：1989-05-12 00:00:00

  • Quantitative analysis of Helicobacter pylori infection in a mouse model.

    abstract：:Progress in elucidating the pathogenesis of Helicobacter pylori gastric infection and in developing an H. pylori vaccine will be aided by an animal model in which H. pylori can be reliably detected. To validate the use of the mouse model of H. pylori infection, we determined the susceptibility of three inbred strains ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(00)00215-5

    authors： Smythies LE,Chen JA,Lindsey JR,Ghiara P,Smith PD,Waites KB

    更新日期：2000-08-28 00:00:00

  • 51Cr-labeled human hepatocytes as target cells for cytotoxicity mediated by freshly isolated liver-infiltrating lymphocytes.

    abstract：:Previous studies on lymphocyte cytotoxicity against autologous human hepatocytes had been studied using Terasaki plates, in which dead hepatocytes after incubation with lymphocytes were counted visually. No studies with 51Cr-labeled human hepatocytes as targets have been reported, although it can give us more objectiv...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(93)90277-e

    authors： Shimizu Y,Hata K,Herberman RB,Whiteside TL

    更新日期：1993-08-26 00:00:00

  • An improved method for immobilizing IgG antibodies on protein A-agarose.

    abstract：:This report describes a modification of a procedure developed by others for crosslinking IgG to protein A which itself is covalently linked to a gel support. Earlier immunoaffinity columns were described as having large antigen-binding capacities and stability under a variety of elution conditions. The present data sh...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(90)90071-3

    authors： Sisson TH,Castor CW

    更新日期：1990-03-09 00:00:00

  • Rapid, sensitive detection of plasma IL-1 by extraction and bioassay.

    abstract：:A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery. ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(88)90399-7

    authors： Series JJ,Shapiro D,Cameron I,Smith J,Fraser WD,Garden OJ,Shenkin A

    更新日期：1988-04-06 00:00:00

  • The use of supernatants from neuraminidase and galactose oxidase (NAGO)-treated lymphocytes as a source of T cell growth factor.

    abstract：:Human peripheral blood or tonsil lymphocytes produce T cell growth factor (TCGF), when activated with neuraminidase (NA) and galactose oxidase (GO). Partial purification of NAGO-TCGF on Sepharose G-100 columns gave a TCGF-active fraction within the same molecular weight range as the conventional lectin-induced TCGF (a...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(83)80004-0

    authors： Berzins T,Wikén M,Hellström U,Perlmann P

    更新日期：1983-10-28 00:00:00

  • Contaminant bands on SDS-polyacrylamide gel electrophoresis are recognised by antibodies in normal human serum and saliva.

    abstract：:Contamination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis samples has commonly produced artefactual bands when sensitive detection systems, such as silver staining or immunoblotting procedures, have been used. Evidence suggests that these bands originate from human skin contamination of reagents and ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(88)90090-7

    authors： Riches PG,Polce B,Hong R

    更新日期：1988-05-25 00:00:00

  • In vivo activation of quiescent B cells by anti-immunoglobulin. II. Production of lysozyme-specific monoclonal antibodies of desired isotype.

    abstract：:We wished to determine whether injection of mice with anti-isotype antibody would be a means to regulate in vivo isotype expression and to obtain hybridomas secreting monoclonal antibodies (mAbs) of desired antigen specificity and isotype. Treatment with a rat mAb (7D2) reactive with both the IgG2a and IgG2b isotypes ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(88)90007-5

    authors： Metzger DW,Walker WS

    更新日期：1988-02-24 00:00:00

  • Factors influencing the measurement of oestrone sulphate by dipstick particle capture immunoassay.

    abstract：:Factors influencing the performance of a dipstick competitive particle capture immunoassay (PCI) for the steroid oestrone sulphate (OS) were investigated. Appropriate 'blocking' of the nitrocellulose dipstick membrane was necessary for the upward flow of microsphere particles. Traditional protein blocking agents inclu...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(02)00280-6

    authors： Henderson K,Stewart J

    更新日期：2002-12-01 00:00:00

  • Affinity panning of peptide libraries using anti-streptokinase monoclonal antibodies: selection of an inhibitor of plasmin(ogen) active site.

    abstract：:To select sequences complementary to their binding sites, two anti-streptokinase (SK) monoclonal antibodies (mAbs), A4.5 and A5.5, were used in biopanning of 15-mer and hexamer phage-displayed peptide libraries, respectively. mAb A4.5 inhibits the catalytic activity of streptokinase-plasminogen activator complex (SKPA...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(02)00183-7

    authors： Parhami-Seren B,Krudysz J,Tsantili P

    更新日期：2002-09-15 00:00:00

  • The ability of horse serum to support an in vitro antibody response.

    abstract：:It was found that 10% horse serum (HrS) could be used to support an in vitro antibody response to SRBC. It could replace the fetal calf serum (FCS) in both the culture medium and the nutritional feed. The response could be improved by the addition of 2-mercaptoethanol or by including both FCS (5%) and HrS (5%) in the ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(80)90042-3

    authors： Levy EM

    更新日期：1980-01-01 00:00:00

  • Sensitive ELISA for IgG and IgM anti-albumin autoantibodies not influenced by HBsAg-associated polyalbumin receptors.

    abstract：:An enzyme immunoassay for the detection of IgG and IgM anti-polymerized albumin autoantibodies (AAA) is described. It was found that polyalbumin receptors on HBsAg particles interfere in the detection of IgG AAA when polymerized human albumin (pHSA), but not polymerized bovine albumin (pBSA), is used as coating antige...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(85)90484-3

    authors： Nardiello S,Pizzella T,Russo M,Galanti B

    更新日期：1985-01-21 00:00:00

  • Characteristics of chemically aggregated IgM in an immunoglobulin class-specific immune complex assay.

    abstract：:Immune complexes have been implicated in the pathogenesis of a number of infectious diseases. The predominant immunoglobulin class associated with circulating immune complexes is IgG, although immune complexes containing IgM have been described. The role of IgM immune complexes in disease pathogenesis has been difficu...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(87)80005-4

    authors： Margolis HS,Nainan OV,Fields HA,Bradley DW

    更新日期：1987-08-24 00:00:00

  • Automatic detection of hypoxia in renal tissue stained with HIF-1alpha.

    abstract：OBJECTIVE:The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD:17 images of renal tissues from male rats of Winstar lineage; overall, there we...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2017.02.005

    authors： Buzin AR,Macedo ND,De Araujo IB,Nogueira BV,de Andrade TU,Endringer DC,Lenz D

    更新日期：2017-05-01 00:00:00

  • Stability of human lymphocyte differentiation antigens when stored at room temperature.

    abstract：:The development of monoclonal antibodies to human differentiation antigens and cytofluorographs have added a new dimension to immune monitoring. However, the technology is such that regionalization will most likely occur. We have demonstrated that human lymphocyte antigens OKT3, OKT4, OKT8 and OKT11 are stable in bloo...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(83)90179-5

    authors： Shield CF 3rd,Marlett P,Smith A,Gunter L,Goldstein G

    更新日期：1983-09-16 00:00:00

  • Generation of peptide-specific CD8+ T cells by phytohemagglutinin-stimulated antigen-mRNA-transduced CD4+ T cells.

    abstract：:Functional analysis of antigen-specific CD8(+) T cells is important for understanding the immune response in various immunological disorders. To analyze CD8(+) T cell responses to a variety of antigens with no readily defined peptides available, we developed a system using CD4(+) phytohemagglutinin (PHA) blasts transd...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2006.05.009

    authors： Naota H,Miyahara Y,Okumura S,Kuzushima K,Akatsuka Y,Hiasa A,Kitano S,Takahashi T,Yuta A,Majima Y,Shiku H

    更新日期：2006-07-31 00:00:00

  • Rapid and highly efficient gene transfer into natural killer cells by nucleofection.

    abstract：:Natural killer (NK) cells are important mediators of virus- and tumor-specific immune responses. The transfection of genes into NK cells has been proven difficult and so far requires infection with virus-based vectors. Here, the application of a novel nonviral, electroporation-based gene transfer method is described f...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(02)00431-3

    authors： Trompeter HI,Weinhold S,Thiel C,Wernet P,Uhrberg M

    更新日期：2003-03-01 00:00:00

  • Rosétte formation between human b lymphocytes and zymosan-C3 complexes using different complement sources.

    abstract：:Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not di...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(76)90195-2

    authors： Kajdacsy-Balla AA,Mendes NF

    更新日期：1976-01-01 00:00:00

  • Ethidium bromide in the detection of antibodies to DNA and of circulating DNA by two-stage counterimmunoelectrophoresis.

    abstract：:In an attempt to overcome the limitations of counterimmunoelectrophoresis in the detection of precipitating anti-DNA antibodies or circulating DNA, ethidium bromide has been used to increase the visibility of the precipitin lines and to confirm their specificity. ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(85)90289-3

    authors： Riboldi P,Asero R

    更新日期：1985-12-17 00:00:00

  • Neutrophil CD64 expression as an important diagnostic marker of infection and sepsis in hospital patients.

    abstract：INTRODUCTION:Infection and sepsis are major health problems. Therefore, the need for improved diagnostic indicators, as well as for better therapeutic monitors in the treatment of infection, remains, since the current diagnostic tools have low specificity and passed through minimal changes in the last two decades. OBJ...

    journal_title：Journal of immunological methods

    pub_type： 信件

    doi：10.1016/j.jim.2014.07.011

    authors： Farias MG,de Lucena NP,Dal Bó S,de Castro SM

    更新日期：2014-12-01 00:00:00

  • Potential roles of recombinant acetylcholine receptor α subunit 1-211 in immunoadsorbent and DNA immunization.

    abstract：:The open reading frame of the α-subunit (amino acids 1-211) of human muscle nicotinic acetylcholine receptor (hAChR(α211)) was inserted into eukaryotic expression vector of pPIC9K to form a recombinant plasmid. After transformation and expression, the target protein rhAChR(α211) (recombinant hAChR(α211)) was secretive...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2011.04.015

    authors： Niu L,Guo C,Hao Z,Yuan J,Li Z

    更新日期：2011-09-30 00:00:00

  • Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.

    abstract：:Goat anti-mouse antibodies conjugated with colloidal 40 nm gold particles (G40) were used as secondary layers to stain human T lymphocytes in an attempt to extend the polarized light epi-illumination microscopic technique to flow cytometric multiparameter analysis. G40-labelled T cells were further stained with phycoe...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(87)90211-0

    authors： Festin R,Björklund B,Tötterman TH

    更新日期：1987-07-16 00:00:00

  • A fluorescent in situ hybridization method in flow cytometry to detect HIV-1 specific RNA.

    abstract：:In HIV+ patients, the presence of HIV-RNA in plasma and circulating cells has been reported to be a marker of progression but the percentage of transcriptionally active infected cells remains unclear. We have developed a reliable fluorescent in situ hybridization method for the detection of HIV specific RNA by flow cy...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(96)00070-1

    authors： Borzì RM,Piacentini A,Monaco MC,Lisignoli G,Degrassi A,Cattini L,Santi S,Facchini A

    更新日期：1996-06-21 00:00:00

  • Development of an ultra-sensitive single molecule counting assay for the detection of interleukin-13 as a marker for asthmatic severity.

    abstract：OBJECTIVE:Interleukin-13 (IL-13) has been difficult to quantify within human serum due to low abundance. Available assays have not been sensitive enough to detect IL-13 at the femtogram level. Thus, there are inconsistencies within the published literature as to IL-13 concentrations in normal or disease populations. To...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2015.08.006

    authors： Gaye B,Sikkema D,Lee TN

    更新日期：2015-11-01 00:00:00

  • Cell-specific heterogeneity in sensitivity of phosphatidylinositol-anchored membrane antigens to release by phospholipase C.

    abstract：:Cell surface antigens thought to be linked to the membrane via phosphatidylinositol (PI) are incompletely, and variably, released by treatment with phosphatidylinositol-specific phospholipase C (PI-PLC). The basis for this was investigated with cloned tumor cell lines and PI-PLCs isolated from two species of bacteria....

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(88)90386-9

    authors： Low MG,Stiernberg J,Waneck GL,Flavell RA,Kincade PW

    更新日期：1988-10-04 00:00:00

  • Some characteristics of natural cytostatic mouse splenocytes.

    abstract：:Murine B16 melanoma cells and metastasis variants of this tumor are resistant to NK activity mediated by normal splenocytes. The B16 cells are, however, sensitive to splenocyte-mediated cytostasis. Cytostasis was measured by a [125I]UDR incorporation-inhibition [(125I]UDR I-I) assay. The main characteristics of the [1...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(81)90066-1

    authors： Ehrlich R,Efrati M,Witz IP

    更新日期：1981-01-01 00:00:00

  • Typing of the HLA-DRB3 gene by temperature gradient gel electrophoresis. Prediction of the resolution of four allelic fragments by computational simulation of DNA melting.

    abstract：:Four alleles are currently recognised at the HLA-DRB3 locus (DRB3*0101, DRB3*0201, DRB3*0202 and DRB3*0301). We studied whether 271 bp fragments of the polymorphic second exon, which were prepared using the polymerase chain reaction, could be typed using temperature gradient gel electrophoresis. Thermal stability curv...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(94)90063-9

    authors： Chen M,März W,Usadel KH,Scheuermann EH,Boehm BO

    更新日期：1994-02-10 00:00:00

  • The effect of antigen suicide on numbers of cells binding defined antigenic determinants.

    abstract：:Lymph node cells from guinea pigs immunized to oxidized ferredoxin (O-Fd) were treated in an antigen suicide procedure designed to inactivate lymphocytes binding the haptenic peptide determinants of the ferredoxin molecule. O-Fd-induced DNA synthesis and cells binding either peptide determinant were examined both befo...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(76)90160-5

    authors： Pearson T,Levy J,Kilburn D,Fairhurst K

    更新日期：1976-03-01 00:00:00

  • Use of the periodate oxidation coupling method for the detection of antibody and antibody-producing cells specific for staphylococcal lipoteichoic acid.

    abstract：:The periodate oxidation and chromium chloride coupling methods were compared for their ability to sensitize indicator erythrocytes with staphylococcal lipoteichoic acid (LTA) for the detection of specific antibody. Erythrocytes, sensitized with periodiate-activated lipoteichoic acid, were found to be superior for use ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(80)90070-8

    authors： Beining PR,Flannery GM,Caldes G,Prescott B,Baker PJ

    更新日期：1980-01-01 00:00:00

  • Depletion of monocytes from human peripheral blood leucocytes by passage through sephadex G-10 columns.

    abstract：:Passage of human peripheral blood leucocytes through Sephadex G-10 columns results in a complete depletion of monocytes. Microscopic examination and ingestion of latex particles failed to reveal any monocytes in the column-passed cells. The study of surface markers shows a slight enrichment of T-cells and a decrease o...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(78)90043-1

    authors： Alonso CM,Bernabé RR,Moreno E,Diaz de Espada F

    更新日期：1978-01-01 00:00:00