Abstract:
:Immune complexes have been implicated in the pathogenesis of a number of infectious diseases. The predominant immunoglobulin class associated with circulating immune complexes is IgG, although immune complexes containing IgM have been described. The role of IgM immune complexes in disease pathogenesis has been difficult to characterize due to the lack of a reliable in vitro model. Immunoglobulins aggregated with bis-diazotized benzidine (BDB) are known to function as model immune complexes. We have developed an IgM immune complex using BDB-aggregated IgM which can be used as a reference in a conglutinin-based immune complex assay. Using this assay system, humans and chimpanzees with acute hepatitis A were found to have circulating immune complexes that contained IgM as the predominant antibody.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Margolis HS,Nainan OV,Fields HA,Bradley DWdoi
10.1016/s0022-1759(87)80005-4subject
Has Abstractpub_date
1987-08-24 00:00:00pages
23-31issue
1eissn
0022-1759issn
1872-7905pii
S0022-1759(87)80005-4journal_volume
102pub_type
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