Abstract：
:We have developed and validated a process-specific immunoligand assay based on the Threshold system for the quantification of residual host cell proteins (HCPs) in a recombinant subunit vaccine candidate against the human respiratory syncytial virus (hRSV). The industrial process of this vaccine produced in Escherichia coli, involved five chromatography steps for the production of clinical-grade batches. The clearance of non-product-related protein throughout the purification process was documented by the evaluation of the HCP content in the chromatographic fractions at each step of the downstream processing. The assay had a detection limit of 0.5 ng/ml of HCP equivalent to 10 parts per million (ppm). The quantification limit was 1.3 ng/ml of HCP, giving a sensitivity range of the assay of 10 to 30 ppm. To our knowledge, this is the first sensitive HCP assay reported for a vaccine.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Dagouassat N,Haeuw JF,Robillard V,Damien F,Libon C,Corvaïa N,Lawny F,Nguyen TN,Bonnefoy JY,Beck Adoi
10.1016/s0022-1759(01)00321-0keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
151-9issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175901003210journal_volume
251pub_type
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