Abstract:
:A polymorphism at position 559 in the cDNA of the FCGR3A gene encoding the FcgammaRIIIa generates two allotypes with either a valine (Val) or a phenylalanine (Phe) at amino acid position 158. This polymorphism is of major importance in immunopathology and in pharmacogenetics, especially monoclonal antibody treatments. In this study, we report a single-step and single-tube method for FCGR3A-158V/F genotyping by real-time multiplex allele-specific PCR and melting curve analysis in the presence of SYBR Green I fluorescent dye. Results obtained from 113 samples showed 100% accuracy compared to those obtained with a conventional PCR-based allele-specific restriction assay. Although this method requires expensive equipment, it is inexpensive in terms of consumables. It is also very rapid, reliable and suitable for large-scale analysis.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Dall'Ozzo S,Andres C,Bardos P,Watier H,Thibault Gdoi
10.1016/s0022-1759(03)00123-6keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
185-92issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175903001236journal_volume
277pub_type
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