A simple and efficient method for the production of human gamma interferon.

abstract：:The cell mediated immune response to herpes simplex virus was assessed by a modification of the leukocyte migration inhibition test, developed to increase its sensitivity and correlated with the in vitro stimulation of lymphocytes. The introduction of a preincubation step allowed us to demonstrate significant inhibiti...

journal_title：Journal of immunological methods

authors： Russell AS,Kaiser J,Lao VS

更新日期：1976-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) accounts for >700,000 deaths worldwide, largely related to poor rates of diagnosis. Our previous work identified glycoproteins with increased levels of fucosylation in HCC. Plate-based assays to measure this change were compromised by increased levels of heterophilic antibodies with glyc...

journal_title：Journal of immunological methods

authors： Wang M,Shen J,Herrera H,Singal A,Swindell C,Renquan L,Mehta A

更新日期：2018-11-01 00:00:00

abstract：:There is a need for simple and sensitive assays to assess innate and adaptive immune responses to microbial agents and vaccines. Herein, we describe a whole blood method allowing to measure the induction of cytokine synthesis at the mRNA level. The originality of this method consists in the combination of PAXgene tube...

journal_title：Journal of immunological methods

authors： Stordeur P,Zhou L,Byl B,Brohet F,Burny W,de Groote D,van der Poll T,Goldman M

更新日期：2003-05-01 00:00:00

abstract：:To date, the applications of bispecific antibodies in immunoassay and immunocytochemical procedures have been directed at uniting two different biomolecules through the binding of epitopes on each respective substance. In this study, bispecific antibodies were constructed in which both binding sites were directed to t...

journal_title：Journal of immunological methods

authors： Cook AG,Wood PJ

更新日期：1994-05-16 00:00:00

abstract：:Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action implicated in the clinical efficacy of several therapeutic antibodies. In vitro ADCC assays employing effector cells capable of inducing lysis of target cells bound by antibodies are routinely performed to support the research and...

journal_title：Journal of immunological methods

authors： Chung S,Lin YL,Reed C,Ng C,Cheng ZJ,Malavasi F,Yang J,Quarmby V,Song A

更新日期：2014-05-01 00:00:00

abstract：:The oxidative burst generation capacity of granulocytes can be reliably detected by a flow cytometric procedure using lysed whole blood, dihydrorhodamine 123 (DHR), and phorbol myristate acetate (PMA). This assay is used to detect chronic granulomatous disease (CGD) and the CGD carrier state. To assess the feasibility...

journal_title：Journal of immunological methods

authors： Prince HE,Lapé-Nixon M

更新日期：1995-12-15 00:00:00

abstract：:Monoclonal antibodies can now be generated against a wide variety of antigens. However, a potentially useful monoclonal antibody may be of the wrong class or subclass for a particular task. Antibodies of the desired class can be obtained by identifying rare subclones in which the variable region has been rearranged to...

journal_title：Journal of immunological methods

authors： Spira G,Bargellesi A,Teillaud JL,Scharff MD

更新日期：1984-11-30 00:00:00

abstract：:Incorporation of control valves into a previously described device enabled us to regulate the formation of 8 suction blisters on the upper surface of the forearm in adult human volunteers. After the removal of the raised epidermis and blister fluid, uniform areas of denuded dermis were obtained by placing hollow adhes...

journal_title：Journal of immunological methods

authors： Fernandes AC,Anderson R,Ras GJ

更新日期：1985-11-07 00:00:00

abstract：:Conditions for the reproducible measurement of thymic hormonal effect on the functional activity of human peripheral blood lymphocytes (PBL) were determined using a mixed lymphocyte-tumor culture assay. Three thymic hormonal preparations (thymopoietin, thymic humoral factor and TP-1) were tested by this assay and foun...

journal_title：Journal of immunological methods

authors： Shoham J,Eshel I

更新日期：1980-01-01 00:00:00

abstract：:Affinity chromatography and subsequent ion-exchange chromatography of pooled anti-benzylpenicilloyl (anti-BPO) hyperimmune sera separated 5 different anti-BPO IgG fractions as described in the preceding paper. These fractions were tested for activities in passive hemagglutination (PHA), passive immune hemolysis (PIH),...

journal_title：Journal of immunological methods

authors： Stemberger H,Scheiner O,Wiedermann G,Kraft D,Förster O

更新日期：1978-01-01 00:00:00

abstract：:An immunoassay is described which allows the detection of glycosphingolipid (GSL) antigens on high performance thin layer chromatograms (HPTLC). The method involves: (1) the separation of GSL on HPTLCs; (2) incubation with specific antibodies against carbohydrate structures of GSL, and (3) the detection of specificall...

journal_title：Journal of immunological methods

authors： Bethke U,Müthing J,Schauder B,Conradt P,Mühlradt PF

更新日期：1986-05-01 00:00:00

abstract：:Anti-melatonin antibodies of high titer and good sensitivity were obtained by coupling melatonin to bovine serum albumin with formaldehyde and by immunizing rabbits with multifocal intra-dermal injections. A systematic study of cross-reactions with 14 compounds (indole, aromatic and imidazole derivatives) showed that ...

journal_title：Journal of immunological methods

authors： Lemaitre BJ,Hartmann L

更新日期：1980-01-01 00:00:00

abstract：:A microtechnique to study lymphocyte stimulation in solid cultures by autoradiography is described. Using this technique at a concentration of 2 x 10(6) lymphocytes/chamber, it was found that the percentage of proliferating cells was 8.4% with anti-lymphocyte serum, 3.9% with calcium ionophore A23187, 2.5% with phytoh...

journal_title：Journal of immunological methods

authors： Kondracki E

更新日期：1979-01-01 00:00:00

abstract：:Rabbit immunoglobulin G (RIgG) was reduced with dithioerythritol and analyzed by high performance liquid chromatography. A quantitative method for determining the percentage of reduced half-molecules in the mixture was developed. An acetic acid concentration-dependent rate of dissociation of reduced half-molecules was...

journal_title：Journal of immunological methods

authors： Layne WW,Kassis AI,Van den Abbeele A,Kinsey BM,Adelstein SJ

更新日期：1987-02-11 00:00:00

abstract：:Pre-S2-coded sequences of the hepatitis B virus (HBV) represent important serological markers of HBV infection and elicit the antibodies essential for recovery from type B hepatitis. Monoclonal antibodies (McAbs) directed against two non-overlapping epitopes (pre-S2a and pre-S2b) of the pre-S2 protein of HBV were used...

journal_title：Journal of immunological methods

authors： Budkowska A,Dubreuil P,Riottot MM,Briantais MJ,Pillot J

更新日期：1987-02-26 00:00:00

abstract：:A rapid and simple immunostick immunosorbent ELISA has been developed for the diagnosis of myasthenia gravis. It is based on coating immunosticks with the major myasthenia gravis autoantigens acetylcholine receptor (AChR) or MuSK and it can be performed in <1 h, in a non-specialized examination place by non-skilled pe...

journal_title：Journal of immunological methods

authors： Trakas N,Tzartos SJ

更新日期：2018-09-01 00:00:00

abstract：:Cytolytic T cells (CTL) play a critical role in providing protection against the liver stage of malaria infection. Previous investigations have shown that induction of CTL against peptide or proteins can be achieved by attachment of lipids. In the present study, we used the Plasmodium berghei circumsporozoite protein ...

journal_title：Journal of immunological methods

authors： Verheul AF,Udhayakumar V,Jue DL,Wohlhueter RM,Lal AA

更新日期：1995-06-09 00:00:00

abstract：:Interferon gamma (IFNγ) is a cytokine involved in many anti-viral and immunoregulatory processes. One of the major mechanisms through which IFNγ exerts these effects is by inducing expression of indoleamine 2,3 dioxygenase-1 (IDO1), an enzyme that catalyses the first, rate-limiting step of the kynurenine pathway. In t...

journal_title：Journal of immunological methods

authors： Jusof FF,Khaw LT,Ball HJ,Hunt NH

更新日期：2013-08-30 00:00:00

abstract：BACKGROUND:In light of the HIV pandemic, significant strides have been made in improving treatment options for patients. Technologies to monitor the progress of a patient on such treatment have therefore also been scaled up. Immune activation as measured by CD38 mean fluorescence intensity (MFI) on CD8 T cells has been...

journal_title：Journal of immunological methods

authors： Moodley K,Coetzee LM,Glencross DK

更新日期：2012-04-30 00:00:00

abstract：:Hybridomas secreting monoclonal antibodies specific for hemoglobin nonenzymatically glycated in the non-A1c position were produced by fusion of SP 2/0 myeloma cells with spleen cells from BALB/c mice immunized with nonenzymatically glycated hemoglobin prepared from human erythrocytes. Wells containing hydridomas secre...

journal_title：Journal of immunological methods

authors： Steward LA,Wu VY,Shea E,Cohen MP

更新日期：1991-07-05 00:00:00

abstract：:An easy rapid method for layering mammalian blood on Ficoll-Isopaque gradients is described. ...

journal_title：Journal of immunological methods

authors： Wilson BJ,Kocvara H

更新日期：1975-11-01 00:00:00

abstract：:Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not cause cell activat...

journal_title：Journal of immunological methods

authors： Abewe H,Deshmukh S,Mukim A,Beliakova-Bethell N

更新日期：2020-01-01 00:00:00

abstract：:The production of antibodies is accompanied by a slight excess of synthesis of κ and λ immunoglobulin light chains; small amounts of them are released in the peripheral blood and can also be found in various body fluids, such as synovial fluid, cerebrospinal fluid, urine and saliva. They are rapidly filtered by the gl...

journal_title：Journal of immunological methods

authors： Basile U,Gulli F,Gragnani L,Napodano C,Pocino K,Rapaccini GL,Mussap M,Zignego AL

更新日期：2017-12-01 00:00:00

abstract：:To compare the responses of individual neutrophils to chemoattractants, migration pathway data were obtained using TAXIScan, an optically accessible/horizontal apparatus in which a concentration gradient is established reproducibly for a given stimulus. The observed linear-mode trajectory pattern of neutrophils toward...

journal_title：Journal of immunological methods

authors： Yamauchi A,Degawa-Yamauchi M,Kuribayashi F,Kanegasaki S,Tsuchiya T

更新日期：2014-02-01 00:00:00

abstract：:The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important to many areas of immunological research. The phenotype and function of immune cell populations at the site of infection is a key determinant of pathogen clearance. However, infections with hel...

journal_title：Journal of immunological methods

authors： Webster HC,Andrusaite AT,Shergold AL,Milling SWF,Perona-Wright G

更新日期：2020-02-01 00:00:00

abstract：:Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to...

journal_title：Journal of immunological methods

authors： Jain K,Jain NK

更新日期：2015-07-01 00:00:00

abstract：:Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation ...

journal_title：Journal of immunological methods

authors： Miyakawa S,Okui T,Shiraishi T,Yoshihara T,Hirayama M,Satomi Y,Hamada T,Nishida M,Akimoto C,Sato S

更新日期：2020-05-01 00:00:00

abstract：:Functional analysis of antigen-specific CD8(+) T cells is important for understanding the immune response in various immunological disorders. To analyze CD8(+) T cell responses to a variety of antigens with no readily defined peptides available, we developed a system using CD4(+) phytohemagglutinin (PHA) blasts transd...

journal_title：Journal of immunological methods

authors： Naota H,Miyahara Y,Okumura S,Kuzushima K,Akatsuka Y,Hiasa A,Kitano S,Takahashi T,Yuta A,Majima Y,Shiku H

更新日期：2006-07-31 00:00:00

abstract：:After B lymphocyte depletion, blood lymphocytes (PBMC) from certain individuals will generate substantial quantities of immunoglobulin (Ig) when cultured with pokeweed mitogen (PWM). The present investigations were aimed at analysing the cell subset in these T cell preparations, that was responsible for Ig production ...

journal_title：Journal of immunological methods

authors： Lobo PI

更新日期：1984-11-16 00:00:00

abstract：:A fragment of antigen binding (Fab) surface display system was developed using a glycoengineered Pichia pastoris host strain genetically modified to secrete glycoproteins with mammalian mannose-type Man(5)GlcNAc(2) N-linked glycans. The surface display method described here takes advantage of a pair of coiled-coil pep...

journal_title：Journal of immunological methods

authors： Lin S,Houston-Cummings NR,Prinz B,Moore R,Bobrowicz B,Davidson RC,Wildt S,Stadheim TA,Zha D

更新日期：2012-01-31 00:00:00