The identification of monoclonal class switch variants by sib selection and an ELISA assay.

abstract：:An enzyme-linked immunosorbent assay (ELISA) has been developed for the quantitation of part-per-million levels of the most probable E. coli polypeptide (ECP) contaminants of E. coli produced biosynthetic human growth hormone (hGH). The antibody preparation, used for both coat and conjugate in this ELISA, was demonstr...

journal_title：Journal of immunological methods

authors： Anicetti VR,Fehskens EF,Reed BR,Chen AB,Moore P,Geier MD,Jones AJ

更新日期：1986-07-24 00:00:00

abstract：:IL-21 is a pleiotropic cytokine that plays a key role in modulating inflammatory responses, including the promotion of autoimmune diseases. Several groups have quantitated circulating levels of IL-21 in plasma and serum samples using various commercial ELISAs. We determined, however, that the most commonly used commer...

journal_title：Journal of immunological methods

authors： Poorbaugh J,Samanta T,Bright SW,Sissons SE,Chang CY,Oberoi P,MacDonald AJ,Martin AP,Cox KL,Benschop RJ

更新日期：2019-03-01 00:00:00

abstract：:This paper describes a new and easy to handle reusable minifermenter for high-density culture of hybridoma and other cells. The culture apparatus is composed of two modules: a 40 ml disposable cell culture and antibody production chamber (the 'production module') and a 550 ml medium reservoir (the 'supply module'). Th...

journal_title：Journal of immunological methods

authors： Falkenberg FW,Weichert H,Krane M,Bartels I,Palme M,Nagels HO,Fiebig H

更新日期：1995-02-13 00:00:00

abstract：:ELISAs for urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) have shown that tumor levels of these molecules are prognostic parameters in breast cancer as well as other types of cancer. These ELISAs measure the total amount of the given component, including preforms, active, inac...

journal_title：Journal of immunological methods

authors： Pedersen AN,Høyer-Hansen G,Brünner N,Clark GM,Larsen B,Poulsen HS,Danø K,Stephens RW

更新日期：1997-04-11 00:00:00

abstract：:This paper reports a new ELISA to measure the level of rabies anti-glycoprotein G antibodies after vaccination. The Platelia Rabies II kit was evaluated on different populations of dogs, cats and foxes. For each target species, sera from naive, unvaccinated and vaccinated animals were tested. Platelia Rabies II result...

journal_title：Journal of immunological methods

authors： Servat A,Feyssaguet M,Blanchard I,Morize JL,Schereffer JL,Boue F,Cliquet F

更新日期：2007-01-10 00:00:00

abstract：:The presence of KIR4.1 antibodies has been proposed to be a characteristic of Multiple Sclerosis (MS). This could have a significant impact on disease management. However, the validation of the initial findings has failed till date. Conflicting results have been attributed to difficulties in isolating the lower-glycos...

journal_title：Journal of immunological methods

authors： Marnetto F,Valentino P,Caldano M,Bertolotto A

更新日期：2017-06-01 00:00:00

abstract：:This study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax antigen. TNF-alpha and IFN-gamma production from memory CD4...

journal_title：Journal of immunological methods

authors： Shinn AH,Bravo NC,Maecker HT,Smith JW

更新日期：2003-11-01 00:00:00

abstract：:A proliferation assay to detect a specific T cell response against HLA class I-binding peptides is reported. To establish the specificity and sensitivity of the assay we used a synthetic peptide derived from the CMV glycoprotein B containing the HLA-A2.1 ligand motif. Lymphocyte proliferation was measured following cu...

journal_title：Journal of immunological methods

authors： Mayer S,Scheibenbogen C,Lee KH,Keilholz W,Stevanovic S,Rammensee HG,Keilholz U

更新日期：1996-10-16 00:00:00

abstract：:We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused. ...

journal_title：Journal of immunological methods

authors： Saravis CA,Zamcheck N

更新日期：1979-01-01 00:00:00

abstract：:A mild procedure for the purification of rabbit Fab' antibodies is described. A small column of normal goat IgG-Sepharose 4B (3 cm x 5mm) was saturated with F(ab')2 prepared from rabbit anti-goat IgG, and anti-goat IgG Fab was eluted from the column by splitting the disulfide bond in the hinge of the F(ab')2 molecule ...

journal_title：Journal of immunological methods

authors： Ishikawa E,Yoshitake S

更新日期：1980-01-01 00:00:00

abstract：:This paper describes procedures for the detection and selection of bacterial colonies expressing antibody fragments of desired antigen specificity. Fab and Fv fragments are detected in a filter assay in which bacterial colonies are grown on a master filter in contract with a second, antigen-coated filter. Ab fragments...

journal_title：Journal of immunological methods

authors： Dreher ML,Gherardi E,Skerra A,Milstein C

更新日期：1991-06-03 00:00:00

abstract：:Systems biology approaches that utilize large genomic data sets hold great potential for deciphering complex immunological process. In this paper, we propose such an approach to derive informative modules and networks from large gene expression data sets. Our approach starts with the clustering of such data sets to de...

journal_title：Journal of immunological methods

authors： Pandey G,Cohain A,Miller J,Merad M

更新日期：2013-01-31 00:00:00

abstract：:We wished to determine whether injection of mice with anti-isotype antibody would be a means to regulate in vivo isotype expression and to obtain hybridomas secreting monoclonal antibodies (mAbs) of desired antigen specificity and isotype. Treatment with a rat mAb (7D2) reactive with both the IgG2a and IgG2b isotypes ...

journal_title：Journal of immunological methods

authors： Metzger DW,Walker WS

更新日期：1988-02-24 00:00:00

abstract：:We have investigated the correlation between results obtained by three different methods (semi-quantitative RT-PCR, ELISA and ELISPOT) used to measure cytokine expression by mouse leukocytes. The production of the cytokines tumour necrosis factor-alpha (TNF alpha), interferon-gamma (IFN gamma) and interleukin-4 (IL-4)...

journal_title：Journal of immunological methods

authors： Favre N,Bordmann G,Rudin W

更新日期：1997-05-12 00:00:00

abstract：:HIV-1 negative regulatory factor (Nef) can inhibit CTL recognition by downregulation of HLA-A and HLA-B on the cell surface. In contrast, HLA-C is not affected by Nef and a growing number of studies demonstrate an important role of HLA-C for the control of HIV-1. So far, only a limited number of HLA-C restricted CTL e...

journal_title：Journal of immunological methods

authors： Stoll A,Bergmann S,Mummert C,Mueller-Schmucker SM,Spriewald BM,Harrer EG,Harrer T

更新日期：2015-03-01 00:00:00

abstract：:A solid-phase radioimmunometric binding assay is described utilizing 125I-labeled protein A for the detection of antibody to human melanoma associated antigens. The novel aspect of this assay is the use of chemically defined spent culture medium of melanoma cells at target antigens previously depleted of fibronectin b...

journal_title：Journal of immunological methods

authors： Morgan AC Jr,Galloway DR,Wilson BS,Reisfeld RA

更新日期：1980-01-01 00:00:00

abstract：:Western blotting is one of the most commonly and widely used methods for identifying the presence of an antigen in biochemical studies. The present study describes a novel 'coupled blotting' approach that allows antigen on dot and Western blots to be simultaneously probed with primary and secondary probing reagents. T...

journal_title：Journal of immunological methods

authors： Sundaram P

更新日期：1996-09-09 00:00:00

abstract：:Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera ven...

journal_title：Journal of immunological methods

authors： Asseman C,Pancré V,Delanoye A,Capron A,Auriault C

更新日期：1994-07-12 00:00:00

abstract：:The major histocompatibility complex (MHC) class I related receptor, the neonatal Fc receptor (FcRn), rescues immunoglobulin G (IgG) and albumin from lysosomal degradation by recycling in endothelial cells. FcRn also contributes to passive immunity by mediating transport of IgG from mother to fetus (human) or newborn ...

journal_title：Journal of immunological methods

authors： Andersen JT,Justesen S,Berntzen G,Michaelsen TE,Lauvrak V,Fleckenstein B,Buus S,Sandlie I

更新日期：2008-02-29 00:00:00

abstract：BACKGROUND:High concentrations of propidium iodide (PI), in combination with fluorescein isothiocyanate (FITC) and R-phycoerythrin (RPE) used for multiparameter DNA flow cytometry (FCM), cause spectral cross-talk into the green fluorescence channel (FL1). We have evaluated the use of post-acquisition software compensat...

journal_title：Journal of immunological methods

authors： Corver WE,Fleuren GJ,Cornelisse CJ

更新日期：2002-02-01 00:00:00

abstract：:A sensitive, simple and reproducible biotin-avidin amplified double antibody immunoassay to quantitate low concentrations of mouse immunoglobulins is described. The assay is a useful technique to measure trace levels of murine monoclonal antibodies in culture supernatants of hybridoma cells metabolically labeled with ...

journal_title：Journal of immunological methods

authors： Ziai MR,Imberti L,Ferrone S

更新日期：1985-10-10 00:00:00

abstract：:The diagnostic and therapeutic applications of antibody single-chain Fv (sFv) fragments often require large amounts of protein that can be problematic and expensive to obtain. Here we report the secretion of two sFv fragments by the yeast Pichia pastoris at levels up to 250 mg/l. Soluble sFv fragments were purified fr...

journal_title：Journal of immunological methods

authors： Eldin P,Pauza ME,Hieda Y,Lin G,Murtaugh MP,Pentel PR,Pennell CA

更新日期：1997-02-14 00:00:00

abstract：:Phycobilisomes are cyanobacterial photosynthetic energy transfer complexes partly composed of phycobiliproteins, proteins that are widely used as conjugable fluorochromes for flow cytometry. The brightness and photostability of phycobiliproteins suggest that intact phycobilisomes could constitute even brighter probes ...

journal_title：Journal of immunological methods

authors： Telford WG,Moss MW,Morseman JP,Allnutt FC

更新日期：2001-08-01 00:00:00

abstract：:The sandwich solid phase radioimmunoassay procedure has been adapted to the serological diagnosis of tuberculosis by the use of disposable plastic haemagglutination trays in which the wells were sensitized with soluble mycobacterial antigen and the subsequent uptake of globulin from test sera detected with radioiodina...

journal_title：Journal of immunological methods

authors： Nassau E,Parsons ER,Johnson GD

更新日期：1975-01-01 00:00:00

abstract：:Human CD1d-restricted natural killer T (NKT) cells, which are postulated to regulate the immune response in several clinical settings, can be activated by alpha-galactosylceramide (alpha-GalCer) presented by CD1d molecules on antigen presenting cells (APCs). Simple methods to quantify NKT function in fresh blood will ...

journal_title：Journal of immunological methods

authors： Fujii S,Shimizu K,Steinman RM,Dhodapkar MV

更新日期：2003-01-15 00:00:00

abstract：:To select sequences complementary to their binding sites, two anti-streptokinase (SK) monoclonal antibodies (mAbs), A4.5 and A5.5, were used in biopanning of 15-mer and hexamer phage-displayed peptide libraries, respectively. mAb A4.5 inhibits the catalytic activity of streptokinase-plasminogen activator complex (SKPA...

journal_title：Journal of immunological methods

authors： Parhami-Seren B,Krudysz J,Tsantili P

更新日期：2002-09-15 00:00:00

abstract：:Many enzyme-linked immunosorbent assays take advantage of immobilized antigens for the identification of antibody binding sites. Generally, the analysis of cellulose membrane-bound B-cell epitopes is currently considered of high utility. We adapted this methodology for the stimulation of a T helper cell hybridoma with...

journal_title：Journal of immunological methods

authors： Otvos L Jr,Pease AM,Bokonyi K,Giles-Davis W,Rogers ME,Hintz PA,Hoffmann R,Ertl HC

更新日期：2000-01-13 00:00:00

abstract：:ADP-ribosylation factors (Arf) are small GTP-binding proteins involved in vesicular transport and the activation of phospholipase D (PLD). The conversion of Arf-GDP to Arf-GTP is promoted in vivo by guanine nucleotide exchange factors such as ARNO or cytohesin-1. In order to examine the expression of ARNO and cytohesi...

journal_title：Journal of immunological methods

authors： Garceau V,Houle MG,Chouinard F,Gagnon S,Harbour D,Naccache PH,Bourgoin SG

更新日期：2001-03-01 00:00:00

abstract：:One of the major functions of mononuclear phagocytes, i.e., monocytes and macrophages, is the phagocytosis and killing of microorganisms. To obtain more insight into the pathogenesis of infectious diseases and to develop new therapies against these diseases, a better understanding of the antimicrobial mechanisms emplo...

journal_title：Journal of immunological methods

authors： Langermans JA,Hazenbos WL,van Furth R

更新日期：1994-09-14 00:00:00

abstract：:In this study, we have developed a simple and efficient technique for the isolation of viable lymphocytes from the epithelium and stroma of small pre-neoplastic squamous intraepithelial lesions (SIL) of the uterine cervix. Following the separation of the epithelium from the stroma using dispase II, both biopsy fragmen...

journal_title：Journal of immunological methods

authors： Jacobs N,Giannini SL,Al-Saleh W,Hubert P,Boniver J,Delvenne P

更新日期：1999-02-01 00:00:00