Abstract：
:A method for determining the affinity and the concentration of mouse monoclonal antibodies against human IgG has been developed. The method comprises two steps. First, monoclonal antibodies are allowed to combine with radioiodinated human IgG. Secondly, mouse monoclonal IgG with and without complexed IgG is precipitated with rabbit IgG against mouse IgG. As the antigen is divalent complexes of varying composition are formed in this system leading to deviations from linearity in plots obtained in commonly employed analytical systems. The theoretical background of these systems has been studied by computer stimulation, and a concentration effect on the formation of immune complexes were demonstrated. The affinities (in terms of the association constants (of 6 monoclonal antibodies were estimated and found to be in the range from 2 X 10(6) M-1 to 5 X 10(8) M-1.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Jacobsen C,Frich JR,Steensgaard Jdoi
10.1016/0022-1759(82)90305-2subject
Has Abstractpub_date
1982-01-01 00:00:00pages
77-88issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(82)90305-2journal_volume
50pub_type
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1983-10-14 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1998-08-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章,评审
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
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