A rapid method for the quantitation of estrogen receptors in small amounts of tissue.

abstract：:A mild procedure for the purification of rabbit Fab' antibodies is described. A small column of normal goat IgG-Sepharose 4B (3 cm x 5mm) was saturated with F(ab')2 prepared from rabbit anti-goat IgG, and anti-goat IgG Fab was eluted from the column by splitting the disulfide bond in the hinge of the F(ab')2 molecule ...

journal_title：Journal of immunological methods

authors： Ishikawa E,Yoshitake S

更新日期：1980-01-01 00:00:00

abstract：:A monoclonal antibody to human IgG was tested with myeloma proteins of the four IgG subclasses. When tested by immunofluorometric assay, enzyme-linked immunosorbent assay, hemagglutination and hemagglutination inhibition assays, the antibody reacted with IgG3 but not with the other three IgG subclasses. When tested by...

journal_title：Journal of immunological methods

authors： Molinaro GA,Eby WC,Reimer C

更新日期：1987-02-11 00:00:00

abstract：:Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was...

journal_title：Journal of immunological methods

authors： Zhao J,Chang W,Liu L,Xing X,Zhang C,Meng H,Gopinath SCB,Lakshmipriya T,Chen Y,Liu Y

更新日期：2021-02-01 00:00:00

abstract：:E. coli O157:H7 is a pathogenic microorganism that has been implicated in numerous cases of foodborne illnesses. A variety of rapid methods exist that show promise for the presumptive detection of this pathogen without the immediate need for incubating test samples for hours to days in microbial enrichment and culture...

journal_title：Journal of immunological methods

authors： Gehring AG,Irwin PL,Reed SA,Tu SI,Andreotti PE,Akhavan-Tafti H,Handley RS

更新日期：2004-10-01 00:00:00

abstract：:Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action implicated in the clinical efficacy of several therapeutic antibodies. In vitro ADCC assays employing effector cells capable of inducing lysis of target cells bound by antibodies are routinely performed to support the research and...

journal_title：Journal of immunological methods

authors： Chung S,Lin YL,Reed C,Ng C,Cheng ZJ,Malavasi F,Yang J,Quarmby V,Song A

更新日期：2014-05-01 00:00:00

abstract：:A new sandwich-type solid-phase enzyme immunoassay (EIA) for carcinoembryonic antigen (CEA) was established by using two selected monoclonal antibodies (MAbs). One MAb, F82-61, which is immobilized on polystyrene beads, reacts with an epitope present on the N-terminal domain (N) of the CEA molecule and the other, F11-...

journal_title：Journal of immunological methods

authors： Nagasaki H,Watanabe S,Kuroki M,Matsumoto Y,Murakami M,Omoda N,Kondo N,Jinnai H,Takaoka H,Matsuoka Y

更新日期：1993-06-18 00:00:00

abstract：:An indirect rosette assay which utilizes erythrocytes coupled with purified antiimmunoglobulin (Ig) antibodies was modified into a microassay for detecting HLA allo-and xenoantibodies. The test, which is performed in microtiter plates, is specific, reproducible and can handle large numbers of samples. As an inhibition...

journal_title：Journal of immunological methods

authors： Indiveri F,Wilson BS,Pellegrino MA,Ferrone S

更新日期：1979-01-01 00:00:00

abstract：:Monitoring recency of infection helps to identify current transmission in vulnerable populations for effective disease control. We have established an in-house avidity based hepatitis C virus (HCV) recency assay based on the Monolisa Anti-HCV PLUS Version 3 ELISA kit for use of dried serum/plasma spots (DS/PS) in orde...

journal_title：Journal of immunological methods

authors： Eshetu A,Hauser A,An der Heiden M,Schmidt D,Meixenberger K,Ross S,Obermeier M,Ehret R,Bock CT,Bartmeyer B,Bremer V,Bannert N

更新日期：2020-04-01 00:00:00

abstract：:Conditions were optimized for measuring the activity of myeloperoxidase (MPO) and the eosinophil peroxidase (EPO) with tetramethylbenzidine (TMB) as the substrate. Detergents caused a small increase in the measured activity of the purified enzymes and were required when isolated neutrophils or eosinophils were assayed...

journal_title：Journal of immunological methods

authors： Bozeman PM,Learn DB,Thomas EL

更新日期：1990-01-24 00:00:00

abstract：:The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historica...

journal_title：Journal of immunological methods

authors： Gene RW,Kumaran J,Aroche C,van Faassen H,Hall JC,MacKenzie CR,Arbabi-Ghahroudi M

更新日期：2015-01-01 00:00:00

abstract：:A solid-phase radioimmunoassay for detection of herpes simplex virus-specific IgG and IgM antibodies in human serum specimens is presented. Virus antigen is adsorbed on polystyrene balls and antibodies which attach to the antigen are detected by 125I-labeled antihuman-gamma or antihuman-mu immunoglobulins. A total of ...

journal_title：Journal of immunological methods

authors： Kalimo KO,Ziola BR,Viljanen MK,Granfors K,Toivanen P

更新日期：1977-01-01 00:00:00

abstract：:Traditionally, the identification and quantification of eosinophils in inflammatory tissues and exudates has been primarily based upon morphologic criteria and manual counting. In this study, we describe a new flow cytometry-based assay to enumerate eosinophils present in murine bronchoalveolar lavage fluid (BAL) and ...

journal_title：Journal of immunological methods

authors： Stevens WW,Kim TS,Pujanauski LM,Hao X,Braciale TJ

更新日期：2007-10-31 00:00:00

abstract：:Dot-immunobinding assay for anti-calmodulin (CaM), and immunoblot assay for CaM have become feasible by proper choice of a fixative, i.e., formaldehyde vapor. The protocol for these simple and convenient immunological procedures is presented. Dot-immunobinding assay could be accomplished in 3 h, and immunoblot assay i...

journal_title：Journal of immunological methods

authors： Natsukari N,Ohta H,Fujita M

更新日期：1989-12-20 00:00:00

abstract：:A rapid method for the simultaneous measurement of neutrophil superoxide generation and beta-glucuronidase release is described. Assay of beta-glucuronidase using a fluorescent substrate is shown to be valid in the presence of reduced or unreduced ferricytochrome C, a prerequisite for the simultaneous assessment of th...

journal_title：Journal of immunological methods

authors： Mandell BF,Ohliger D,Rella J

更新日期：1987-06-26 00:00:00

abstract：:Human CD1d-restricted natural killer T (NKT) cells, which are postulated to regulate the immune response in several clinical settings, can be activated by alpha-galactosylceramide (alpha-GalCer) presented by CD1d molecules on antigen presenting cells (APCs). Simple methods to quantify NKT function in fresh blood will ...

journal_title：Journal of immunological methods

authors： Fujii S,Shimizu K,Steinman RM,Dhodapkar MV

更新日期：2003-01-15 00:00:00

abstract：:Attempts were made to estimate peripheral T-lymphocytes in rabbits by their ability to form rosettes with sheep, human, guinea-pig, rat, mouse and chicken RBC. The reaction was studied under different conditions of pH and temperature. The test was also carried out in the presence or absence of glutaraldehyde. With she...

journal_title：Journal of immunological methods

authors： Vakharia DD,Vakharia MR,Rao SS

更新日期：1977-01-01 00:00:00

abstract：:Phagocyte functions such as chemotaxis and phagocytosis involve the rapid and transient development of cellular polarity. Study of this highly complex spatial and temporal cellular remodelling has been limited by the static nature of immunofluorescence and immunogold microscopy and because biochemical techniques are n...

journal_title：Journal of immunological methods

authors： Bajno L,Grinstein S

更新日期：1999-12-17 00:00:00

abstract：:The structural diversity of the immune response may be considerably restricted by the structure of the hapten used to elicit catalytic antibodies. The ligand-binding mode and the shapes of the binding pockets of hydrolytic antibodies induced to different transition-state analogs that contain an unsubstituted arylphosp...

journal_title：Journal of immunological methods

authors： Golinelli-Pimpaneau B

更新日期：2002-11-01 00:00:00

abstract：:Bovine serum albumin (BSA) was used as a non-amphipathic model protein to be included into iscoms. Pretreatment at an acidic pH (2.5) was used to reveal hydrophobic regions, after which BSA could be integrated. In immunization experiments in mice the BSA iscoms induced long lasting and considerably higher serum antibo...

journal_title：Journal of immunological methods

authors： Morein B,Ekström J,Lövgren K

更新日期：1990-04-17 00:00:00

abstract：:CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and divisionlinked changes in cell behavior. Recently Banks et al. (2011), Luzyanina et al. (2009), Luzyanina et al. (2007), a partial differential equation (PDE) model to describe lymphocyte dynamics in a CFSE proliferat...

journal_title：Journal of immunological methods

authors： Banks HT,Sutton KL,Thompson WC,Bocharov G,Doumic M,Schenkel T,Argilaguet J,Giest S,Peligero C,Meyerhans A

更新日期：2011-10-28 00:00:00

abstract：:The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This chimaera was soluble and abundant (yield = 18 mg/l of bacterial...

journal_title：Journal of immunological methods

authors： Sun S,Lew AM

更新日期：1992-07-31 00:00:00

abstract：:A simple roller bottle was constructed to house three dialysis tubes, each with a capacity of 75 ml. Cells were grown inside the dialysis tubing, which was immersed in ordinary DMEM medium without serum supplement. Cultures of hybridomas at medium or low density (2 X 10(5) cells/ml) could be expanded directly in the d...

journal_title：Journal of immunological methods

authors： Pannell R,Milstein C

更新日期：1992-01-21 00:00:00

abstract：:A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well E...

journal_title：Journal of immunological methods

authors： Kobayashi N,Sugita H,Terada E,Ghoda A,Okudaira H,Ogita T,Miyamoto T

更新日期：1984-10-26 00:00:00

abstract：:Intracellular probes used for oxidative burst evaluation could be a substrate of the multidrug resistance proteins (MDR), which may cause misinterpretation of experimental data. We aimed to study the effect of the culture condition and of the MDR-interfering antioxidant quercetin on free radical measurement in serum d...

journal_title：Journal of immunological methods

authors： Peluso I,Romanelli L,Spagnuolo S,Manafikhi H,Palmery M

更新日期：2014-03-01 00:00:00

abstract：:The amino acid glutamine is an essential nutrient for cells in culture. In aqueous solutions such as liquid culture media, glutamine spontaneously decomposes into ammonia. In this study, we examined the toxicity of ammonia for two different cell lines. In mouse hybridoma cell cultures, viable cell counts were reduced ...

journal_title：Journal of immunological methods

authors： Heeneman S,Deutz NE,Buurman WA

更新日期：1993-11-05 00:00:00

abstract：:An ELISA method for the detection of monoclonal antibodies (MAb) to the T3-T cell antigen receptor (TCR) complex was devised. The T3-TCR complex was solubilised using digitonin and a rat anti-T3 MAb (Campath 3) was used to bind it to an ELISA plate. Normal rat serum was used to block cross-reactivity between the rat M...

journal_title：Journal of immunological methods

authors： Clark DM,Boylston AW

更新日期：1988-01-21 00:00:00

abstract：:The levels of protein A-reactive immunoglobulin (PA-Ig) in foetal bovine serum were measured in commercial batches. For tissue culture media incorporating 10% foetal bovine serum, the levels of bovine PA-Ig were of a similar order to those of mouse monoclonal antibodies produced by hybridomas grown in such media. The ...

journal_title：Journal of immunological methods

authors： Underwood PA,Kelly JF,Harman DF,MacMillan HM

更新日期：1983-05-27 00:00:00

abstract：:Piezoelectric quartz crystals (resonance frequency 10 MHz) were used for an investigation of the immunochemical reaction between 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide and several monoclonal antibodies prepared against 2,4-D. The herbicide was immobilized on the gold electrodes of the crystals silanized with...

journal_title：Journal of immunological methods

authors： Skládal P,Minunni M,Mascini M,Kolár V,Fránek M

更新日期：1994-11-10 00:00:00

abstract：:A method for coating native, non-derivatized, polysaccharide (PS) onto nitrocellulose (NC) for identifying PS-specific antibodies has been developed. The new feature of this method is that PS molecules are vacuum filtered onto NC in their native state by devices that can accommodate NC of different sizes and shapes. P...

journal_title：Journal of immunological methods

authors： Feng SH,Rubinstein LJ,Stein KE

更新日期：1991-03-21 00:00:00

abstract：:A study was undertaken to determine whether large amounts of blood (antiserum) could be rapidly removed from rabbits over a two-month period using a simple suction technique. It was found that half a blood volume could be readily removed each week (over 900 ml/rabbit/54 days) without a single fatality. The rabbits eff...

journal_title：Journal of immunological methods

authors： Nerenberg ST,Zedler P,Prasad R,Biskup NS,Pedersen L

更新日期：1978-01-01 00:00:00