Abstract:
:A new sandwich-type solid-phase enzyme immunoassay (EIA) for carcinoembryonic antigen (CEA) was established by using two selected monoclonal antibodies (MAbs). One MAb, F82-61, which is immobilized on polystyrene beads, reacts with an epitope present on the N-terminal domain (N) of the CEA molecule and the other, F11-39, which is conjugated to horseradish peroxidase (HRP), recognizes an epitope present on the C-terminal domain (B3) of the CEA molecule. The assay consists of incubating 0.05 ml of test serum both with F82-61-coated beads and HRP-labeled F11-39 at room temperature for 2 h. The assay system, referred to as New EIA, showed very homogeneous reactivity with purified CEA preparations from different tumors and could discriminate CEA from four CEA-related normal antigens tested; nonspecific cross-reacting antigen (NCA), NCA-2, normal fecal antigen-1 (NFA-1) and NFA-2. The assessment of technical qualities, such as reproducibility, recovery and dilution tests, demonstrated the excellent performance of New EIA. Serum samples (n = 604) from patients with malignant or non-malignant disease, as well as from healthy individuals, were analyzed by New EIA and by two commercial CEA immunoassays which both showed heterogeneous reactivity with different CEA preparations and reacted to a greater or lesser extent with NCA-2 and NFA-2, and it was found that New EIA significantly increased the sensitivity and specificity of tumor diagnosis as compared with the commercial assays.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Nagasaki H,Watanabe S,Kuroki M,Matsumoto Y,Murakami M,Omoda N,Kondo N,Jinnai H,Takaoka H,Matsuoka Ydoi
10.1016/0022-1759(93)90388-nsubject
Has Abstractpub_date
1993-06-18 00:00:00pages
235-45issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(93)90388-Njournal_volume
162pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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