Abstract:
:CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and divisionlinked changes in cell behavior. Recently Banks et al. (2011), Luzyanina et al. (2009), Luzyanina et al. (2007), a partial differential equation (PDE) model to describe lymphocyte dynamics in a CFSE proliferation assay was proposed. We present a significant revision of this model which improves the physiological understanding of several parameters. Namely, the parameter used previously as a heuristic explanation for the dilution of CFSE dye by cell division is replaced with a more physical component, cellular autofluorescence. The rate at which label decays is also quantified using a Gompertz decay process. We then demonstrate a revised method of fitting the model to the commonly used histogram representation of the data. It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Banks HT,Sutton KL,Thompson WC,Bocharov G,Doumic M,Schenkel T,Argilaguet J,Giest S,Peligero C,Meyerhans Adoi
10.1016/j.jim.2011.08.014subject
Has Abstractpub_date
2011-10-28 00:00:00pages
143-60issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(11)00219-5journal_volume
373pub_type
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