Abstract:
:Intracellular probes used for oxidative burst evaluation could be a substrate of the multidrug resistance proteins (MDR), which may cause misinterpretation of experimental data. We aimed to study the effect of the culture condition and of the MDR-interfering antioxidant quercetin on free radical measurement in serum depleted HCT-8 cells and chick embryo hepatocytes. Serum depletion and/or quercetin affected the traffic of 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) and calcein. The comparative results obtained with 2',7'-dichlorofluorescein (DCF) and with the plasma-membrane probe C11-BODIPY 581/591 [4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid], suggest caution in the use of intracellular probes to evaluate oxidative stress in vitro.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Peluso I,Romanelli L,Spagnuolo S,Manafikhi H,Palmery Mdoi
10.1016/j.jim.2013.12.009subject
Has Abstractpub_date
2014-03-01 00:00:00pages
178-80eissn
0022-1759issn
1872-7905pii
S0022-1759(13)00373-6journal_volume
405pub_type
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