Automation of enzyme-linked immunosorbent assay (ELISA).

abstract：:The Rho family GTPase Cdc42 is a critical regulator of cellular polarization from yeast to man. An analysis of its function in T cell activation is therefore of interest. This analysis poses two substantial challenges, similar to the analysis of many other critical T cell signaling intermediates. First, Cdc42 is requi...

journal_title：Journal of immunological methods

authors： Tskvitaria-Fuller I,Mistry N,Sun S,Wülfing C

更新日期：2007-01-30 00:00:00

abstract：:A method is described for the study of lymphokine-mediated cellular interactions using triple wells, which permits co-culture of cell subpopulations without direct physical contact. The triple wells are constructed by slitting the walls to half height between three adjacent wells of a 96-well microtiter plate. The cel...

journal_title：Journal of immunological methods

authors： Karawajew L,Micheel B,Jung G,Wolf H,Behrsing O

更新日期：1994-07-12 00:00:00

abstract：:To date, the applications of bispecific antibodies in immunoassay and immunocytochemical procedures have been directed at uniting two different biomolecules through the binding of epitopes on each respective substance. In this study, bispecific antibodies were constructed in which both binding sites were directed to t...

journal_title：Journal of immunological methods

authors： Cook AG,Wood PJ

更新日期：1994-05-16 00:00:00

abstract：:This paper describes the application of experimental design techniques to optimize a sensitive ELISA for a hapten molecule with a calibration range of 0-1000 pg/ml. Ten factors that were expected to affect the assay performance were initially screened, followed by factorial experiments to delineate the effects of the ...

journal_title：Journal of immunological methods

authors： Sittampalam GS,Smith WC,Miyakawa TW,Smith DR,McMorris C

更新日期：1996-04-19 00:00:00

abstract：:Etanercept, a recombinant human tumor necrosis factor (TNF) receptor Fc fusion protein is an effective treatment option in adults with rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis or plaque psoriasis and paediatrics with juvenile idiotypic arthritis and plaque psoriasis. Patent expiration in Europ...

journal_title：Journal of immunological methods

authors： Wadhwa M,Bird C,Dilger P,Rigsby P,Jia H,Gross MEB,participants of the study.

更新日期：2017-08-01 00:00:00

abstract：:A simple apparatus for measuring chemotaxis is described which contains thirty chambers per unit. Only very few cells and small volumes of chemotactic factor solution are required per chamber. For satisfactory results the apparatus must be centrifuged briefly prior to incubation. Under these conditions results can be ...

journal_title：Journal of immunological methods

authors： Swanson MJ

更新日期：1977-01-01 00:00:00

abstract：:The techniques of flow cytometry are being increasingly used to classify lymphocytes according to their surface antigens. Accurate analyses depend on the exclusion of non-lymphocytic contaminant cells. Using a fluorescence-activated cell sorter (FACS IV), virtually complete separation of human lymphocytes from monocyt...

journal_title：Journal of immunological methods

authors： Ritchie AW,Gray RA,Micklem HS

更新日期：1983-11-11 00:00:00

abstract：:We describe a simple and efficient non-chromatographic method for the purification of murine IgG3 and IgM monoclonal antibodies (MAbs) which takes advantage of their euglobulin properties. Following filtration, ascitic fluid is dialysed against demineralized water and centrifuged at 22,000 X g for 30 min. The resultin...

journal_title：Journal of immunological methods

authors： García-González M,Bettinger S,Ott S,Olivier P,Kadouche J,Pouletty P

更新日期：1988-06-28 00:00:00

abstract：:The present report describes further improvements in the methodology for antigen-specific T-lymphocyte activation in vitro. Following the experimental protocol, large numbers of enriched T-lymphocytes can be obtained from the draining lymph nodes of immunized mice after purification on a nylon column. These T-cells re...

journal_title：Journal of immunological methods

authors： Kagan J,Ben-Sasson SZ

更新日期：1980-01-01 00:00:00

abstract：:Pathological prion protein (PrPSc) which is a conformational isoform of a host-encoded protein designated (PrPC) serves as a specific marker protein for the immunochemical diagnosis of transmissible spongiform encephalopathies (TSE). The generation of suitable antibodies to PrPSc therefore underlies the specificity an...

journal_title：Journal of immunological methods

authors： Groschup MH,Harmeyer S,Pfaff E

更新日期：1997-08-22 00:00:00

abstract：:Regulatory T cells (Treg) have recently come to the fore in studies of immune regulation, particularly in autoimmune disease and cancer. While there appear to be several distinct subsets of T cells with regulatory function, a population described as natural Treg and characterized by expression of the transcription fac...

journal_title：Journal of immunological methods

authors： Bresatz S,Sadlon T,Millard D,Zola H,Barry SC

更新日期：2007-10-31 00:00:00

abstract：UNLABELLED:In this study the flow-cytometric crossmatch results were compared between fresh cells and cells processed by various cryopreservation and storage methods. Platelets from healthy donors were incubated with 12 sera containing platelet reactive antibodies as well as with 62 control sera from blood donors. Dire...

journal_title：Journal of immunological methods

authors： Döhlinger S,Humpe A,Connor J,Köhler M,Legler TJ

更新日期：2005-02-01 00:00:00

abstract：:A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing....

journal_title：Journal of immunological methods

authors： Trudgett A

更新日期：1976-01-01 00:00:00

abstract：:Nonspecific effects of reagents used to selectively isolate lymphocyte subpopulations are most often analyzed according to their influence on cell viability and proliferation. While characterizing monoclonal anti-human B cell antibodies, we detected consistent changes in B cell function between individual donors, no m...

journal_title：Journal of immunological methods

authors： Munchus MS,Levitt D

更新日期：1984-02-10 00:00:00

abstract：:Fab's with hinges based on the human gamma1 sequence containing 1, 2, or 4 cysteines have been produced by high level Escherichia coli periplasmic secretion, and coupled in vitro by reduction/oxidation to form F(ab')2. We find that the F(ab')2 made with hinges containing 2 or 4 cysteines have a high level (approximate...

journal_title：Journal of immunological methods

authors： Humphreys DP,Vetterlein OM,Chapman AP,King DJ,Antoniw P,Suitters AJ,Reeks DG,Parton TA,King LM,Smith BJ,Lang V,Stephens PE

更新日期：1998-08-01 00:00:00

abstract：:Reactive lymph nodes (LNs) are sites where pMHC-loaded dendritic cells (DCs) interact with rare cognate T cells, leading to their clonal expansion. While DC interactions with T cell subsets critically shape the ensuing immune response, surprisingly little is known on their spatial orchestration at physiologically T ce...

journal_title：Journal of immunological methods

authors： Abe J,Ozga AJ,Swoger J,Sharpe J,Ripoll J,Stein JV

更新日期：2016-04-01 00:00:00

abstract：:Unassociated human beta 2-microglobulin (beta 2m) and histocompatibility peptide associated beta 2-microglobulin produce significantly different inhibition curves in a competitive binding assay with rabbit anti-beta 2-microglobulin. This difference disallows the use of the rabbit antisera for measuring MHC-beta 2-micr...

journal_title：Journal of immunological methods

authors： Gerity E,Conway TP

更新日期：1984-03-30 00:00:00

abstract：:Human tumor infiltrating lymphocytes (TIL) from metastatic melanoma of six patients were grown using a new hollow fiber bioreactor system. After inoculating 0.35-10 X 10(8) TIL into the extra-fiber space (EFS), each Cellmax bioreactor was perfused with AIM-V medium, supplemented with rIL-2. The cells subsequently expa...

journal_title：Journal of immunological methods

authors： Knazek RA,Wu YW,Aebersold PM,Rosenberg SA

更新日期：1990-02-20 00:00:00

abstract：INTRODUCTION:Infection and sepsis are major health problems. Therefore, the need for improved diagnostic indicators, as well as for better therapeutic monitors in the treatment of infection, remains, since the current diagnostic tools have low specificity and passed through minimal changes in the last two decades. OBJ...

journal_title：Journal of immunological methods

authors： Farias MG,de Lucena NP,Dal Bó S,de Castro SM

更新日期：2014-12-01 00:00:00

abstract：:CD3-specific antibodies have shown clinical efficacy in both transplantation and autoimmunity. However, targeting CD3 in this way can lead to T-cell activation and a serious cytokine release syndrome mediated by Fcγ receptor binding. An in vivo mouse model has been developed using severe combined immunodeficient (SCID...

journal_title：Journal of immunological methods

authors： Malcolm SL,Smith EL,Bourne T,Shaw S

更新日期：2012-10-31 00:00:00

abstract：:The short-term kinetics of T cell-mediated cytotoxicity was investigated using a cloning inhibition assay. Murine cytotoxic thymus-derived lymphocytes (CTL) generated in vitro in mixed leukocyte cultures (MLC), were incubated for various periods of time at 37 degrees C with allogeneic mastocytoma target cells. The mix...

journal_title：Journal of immunological methods

authors： Lees RK,MacDonald HR,Sinclair NR

更新日期：1977-01-01 00:00:00

abstract：:This paper examines the parameters necessary for the efficient measurement of anti-Theiler's murine encephalomyelitis virus (TMEV) antibodies in an affinity-dependent manner using a variation of a solid-phase particle concentration fluorescence immunoassay (PCFIA). By allowing antibody to react with fluorochrome-label...

journal_title：Journal of immunological methods

authors： Peterson JD,Kim JY,Melvold RW,Miller SD,Waltenbaugh C

更新日期：1989-04-21 00:00:00

abstract：:A sensitive assay, using [14C]lecithin as a substrate, has been developed for the measurement of phospholipase activity in rat peritoneal polymorphonuclear leukocytes. Cell extracts were found to contain a phospholipase D activity and indirect evidence suggested that eosinophils are responsible for the cleavage of lec...

journal_title：Journal of immunological methods

authors： Lempereur C,Capron M,Capron A

更新日期：1980-01-01 00:00:00

abstract：:The in vitro migration inhibition responses of peripheral blood leukocytes from tuberculin skin test positive and negative donors were tested to validate and determine optimal conditions for the agarose droplet method in the human. In vitro migration inhibition was observed in skin test positive donors in the presence...

journal_title：Journal of immunological methods

authors： Lavergne JA,Harrington JT

更新日期：1978-01-01 00:00:00

abstract：:Laurell crossed immunoelectrophoresis (two-dimensional electroimmunodiffusion) was used to prepare minute amounts of purified parasite antigens complexed with their precipitating antibodies obtained from rabbits. These complexes, emuslified in Freund's complete adjuvant, were then used to prime rabbits for selective p...

journal_title：Journal of immunological methods

authors： Hillyer GV,Cervoni M

更新日期：1978-01-01 00:00:00

abstract：:We have recently shown that the semi-continuous cultivation of a mouse hybridoma line in spinner flasks, with a basal defined medium (BDM) devoid of serum and protein, increases the secretion of the immunoreactive monoclonal antibody (MAb) by a factor of ca. 2.4, compared to culture in flasks with serum-containing med...

journal_title：Journal of immunological methods

authors： Schneider YJ,Lavoix A

更新日期：1990-05-25 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is now becoming an accepted tool for measuring gene expression at the transcriptional level. In this study, a direct comparison between real-time PCR, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunosorbent spot (ELISPOT) assay was performed. When interferon-...

journal_title：Journal of immunological methods

authors： Mena A,Ioannou XP,Van Kessel A,Van Drunen Little-Van Den Hurk S,Popowych Y,Babiuk LA,Godson DL

更新日期：2002-05-01 00:00:00

abstract：:Single chain antibody fragment genes are commonly created by splicing together the immunoglobulin light chain (VL) and heavy chain variable (VH) genes of a monoclonal antibody produced by a hybridoma. Selective PCR amplification of the functional immunoglobulin variable gene rearrangements can be complicated by the ex...

journal_title：Journal of immunological methods

authors： Irani Y,Tea M,Tilton RG,Coster DJ,Williams KA,Brereton HM

更新日期：2008-07-31 00:00:00

abstract：:A study was undertaken to establish conditions of growth to increase C3b receptor synthesis on a human B lymphoblastoid cell line (Raji) by use of cell differentiation effectors. It appears that whereas two polar compounds HMBA (2mM) and Me2SO(2%) have no or little effect, 5 BrdU (30microM) and db cAMP (5 x10(-4) M) a...

journal_title：Journal of immunological methods

authors： Barel M,Charriaut C,Frade R

更新日期：1981-01-01 00:00:00

abstract：:Immune repertoires of T or B cells are very often studied by Complementary Determining Region 3 (CDR3) spectratyping. However, data obtained with this method is usually subject to a biased eye analysis. We developed recently the ISEApeaks software package to retrieve and handle peak data from automated sequencers, fro...

journal_title：Journal of immunological methods

authors： Collette A,Cazenave PA,Pied S,Six A

更新日期：2003-07-01 00:00:00