Detection of antibodies to mycobacterium tuberculosis by solid phase radioimmunoassay.

abstract：:The sensitivity and specificity of immunohistological staining procedures employing horseadish peroxidase labeled antibody were evaluated with the aid of chessboard titrations. Two indirect staining systems, detecting antinuclear antibody and pemphigus intercellular antibody, were examined with reference to indirect i...

journal_title：Journal of immunological methods

authors： Shu S,Albini B

更新日期：1976-01-01 00:00:00

abstract：:Antigen-specific lymphocytes are important in the immune response to viral infection. Peripheral blood mononuclear cells (PBMC) are traditionally used as a source of effector cells in most immunological studies. We described here the use of the bispecific monoclonal antibodies (BSMAB) anti CD3:CD8 (CD3,8) and anti CD3...

journal_title：Journal of immunological methods

authors： Jones N,Agrawal D,Elrefaei M,Hanson A,Novitsky V,Wong JT,Cao H

更新日期：2003-03-01 00:00:00

abstract：:Two micro enzyme immunoassays (microEIA) for circulating immune complexes (CICs) are described. The Raji cell microEIA was similar in sensitivity, reproducibility and specificity to the Raji radioimmunoassay. The F(ab')2 anti-C3 microEIA was comparable to the 2 Raji cell assays. The 2 microEIAs for CICs were used to a...

journal_title：Journal of immunological methods

authors： Jordan SC,Gautier E,Sakai R,Bahn L

更新日期：1985-11-07 00:00:00

abstract：:The attachment of cells to plastic tissue culture plates is a widely used criterion of cell viability in microcytotoxicity assays. When cell suspensions of primary human colon carcinoma and melanoma cells which were of low initial viability (assessed by trypan blue exclusion) were allowed to adhere to tissue culture p...

journal_title：Journal of immunological methods

authors： Pullen GR,Chalmers PJ,Nind AP,Nairn RC

更新日期：1981-01-01 00:00:00

abstract：:C3 nephritic factor (C3NeF) is an autoantibody against the C3 convertase which stabilizes this otherwise inherently labile neoenzyme and induces a continuous activation of the alternative pathway with C3 depletion. NeF is found in patients with membranoproliferative glomerulonephritis and/or partial lipodystrpohy. NeF...

journal_title：Journal of immunological methods

authors： Jelezarova E,Schlumberger M,Sadallah S,Späth PJ,Schifferli JA,Lutz HU

更新日期：2001-05-01 00:00:00

abstract：:IgA antibodies are key immune effectors against invading pathogens but also possess essential immunoregulatory functions. Detecting and quantifying human IgA+ B-cell subsets and secreted IgA molecules is needed for investigating the protective, modulatory and pathophysiologic roles of IgAs. Here, we produced a recombi...

journal_title：Journal of immunological methods

authors： Planchais C,Mouquet H

更新日期：2020-01-01 00:00:00

abstract：:Reactive lymph nodes (LNs) are sites where pMHC-loaded dendritic cells (DCs) interact with rare cognate T cells, leading to their clonal expansion. While DC interactions with T cell subsets critically shape the ensuing immune response, surprisingly little is known on their spatial orchestration at physiologically T ce...

journal_title：Journal of immunological methods

authors： Abe J,Ozga AJ,Swoger J,Sharpe J,Ripoll J,Stein JV

更新日期：2016-04-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) is described that used a combination of 2 solid phases: a microtiter plate covered with a Falcon assay screening test (F.A.S.T.) system lid. By coating each solid phase with a different antigen, it was possible to simultaneously detect 2 different antibodies. The results of...

journal_title：Journal of immunological methods

authors： Ianconescu M

更新日期：1985-02-11 00:00:00

abstract：:An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange s...

journal_title：Journal of immunological methods

authors： Luk JM,Lindberg AA

更新日期：1991-03-01 00:00:00

abstract：:An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinitie...

journal_title：Journal of immunological methods

authors： Kirley TL,Norman AB,Wetzel HN

更新日期：2020-01-01 00:00:00

abstract：:Complement activation is a key component of the pathogenesis of immune-mediated tissue damage in many diseases. Assessment of complement activation in current practice is largely based on the measurement of intact C3 and C4 or the determination of complement haemolytic function. These parameters reflect activation onl...

journal_title：Journal of immunological methods

authors： Porcel JM,Peakman M,Senaldi G,Vergani D

更新日期：1993-01-04 00:00:00

abstract：:This study examined the secretion of IL-1 alpha and IL-1 beta by THP-1 leukemia cells following activation with mezerein and promotion of synthesis by interferon (IFN-gamma). Interleukin-1 (IL-1) was not detected by co-mitogenic thymocyte assays of crude supernates. Isoelectrofocusing of concentrated medium showed tha...

journal_title：Journal of immunological methods

authors： Gaffney EV,Stoner CR,Lingenfelter SE,Wagner LA

更新日期：1989-09-01 00:00:00

abstract：BACKGROUND:ABO subgroups or weaker variants of A or B are group A or B subjects whose erythrocytes give a weak or negative reaction serologically with anti-A or Anti - B antisera respectively. Occurrence of these subgroups may lead to an ABO discrepancy which often puts transfusion services in a quandary. ABO subgroups...

journal_title：Journal of immunological methods

authors： Tiwari AK,Setya D,Arora D,Mehta SP,Aggarwal G,Mitra S

更新日期：2020-12-01 00:00:00

abstract：:A method is presented for measuring the hemolytic activity of undiluted guinea pig serum. Undiluted serum is placed in contact with a column of sensitized sheep red blood cells in an agar matrix in a capillary tube. After incubation, the length of the zone of lysis is measured. Comparison with a standard curve prepare...

journal_title：Journal of immunological methods

authors： Brockman JA,Bauman N

更新日期：1979-01-01 00:00:00

abstract：:The Rho family GTPase Cdc42 is a critical regulator of cellular polarization from yeast to man. An analysis of its function in T cell activation is therefore of interest. This analysis poses two substantial challenges, similar to the analysis of many other critical T cell signaling intermediates. First, Cdc42 is requi...

journal_title：Journal of immunological methods

authors： Tskvitaria-Fuller I,Mistry N,Sun S,Wülfing C

更新日期：2007-01-30 00:00:00

abstract：:Colloidal gold particles coated with rabbit anti-HCG (Au-(anti-HCG)) were used in a spectrophotometric 'sandwich' SPIA for HCG. The effects of several reagent properties and assay variables were investigated in order to optimize the assay. Best results were obtained with 55 nm particles. Optimum assay conditions invol...

journal_title：Journal of immunological methods

authors： Leuvering JH,Thal PJ,Schuurs AH

更新日期：1983-08-26 00:00:00

abstract：:Nonspecific effects of reagents used to selectively isolate lymphocyte subpopulations are most often analyzed according to their influence on cell viability and proliferation. While characterizing monoclonal anti-human B cell antibodies, we detected consistent changes in B cell function between individual donors, no m...

journal_title：Journal of immunological methods

authors： Munchus MS,Levitt D

更新日期：1984-02-10 00:00:00

abstract：OBJECTIVE:The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD:17 images of renal tissues from male rats of Winstar lineage; overall, there we...

journal_title：Journal of immunological methods

authors： Buzin AR,Macedo ND,De Araujo IB,Nogueira BV,de Andrade TU,Endringer DC,Lenz D

更新日期：2017-05-01 00:00:00

abstract：:The accumulation of 2-deoxy-D-[1-3H]glucose, a non-metabolised analogue of glucose provides a quantitative measurement of the state of activation of phagocytic cells. A microassay for 3H-deoxyglucose uptake by human neutrophils and monocytes is described. Optimal conditions for the assay include the use of 5 X 10(5) c...

journal_title：Journal of immunological methods

authors： Seow WK,Smith SE,McCormack JG,Thong YH

更新日期：1987-04-02 00:00:00

abstract：:The 7th International Workshop on Human Leucocyte Differentiation Antigens (HLDA7) studied a number of newly characterised molecules relevant to human leucocyte differentiation and function. The HLDA organisation, which devised and continues to maintain the CD nomenclature, is responsible, under the auspices of IUIS a...

journal_title：Journal of immunological methods

authors： Zola H,Swart B,Boumsell L,Mason DY,IUIS\/WHO Subcommittee.

更新日期：2003-04-01 00:00:00

abstract：:Boronated antibodies have already been evaluated as agents in neutron capture therapy. Because the boronation procedure may alter the properties of the antibody it is important to study the immunoreactivity of the conjugated antibody before in vivo use. In our studies of two dextran-boronated monoclonal antibodies, an...

journal_title：Journal of immunological methods

authors： Pettersson ML,Courel MN,Girard N,Abraham R,Gabel D,Thellier M,Delpech B

更新日期：1990-01-24 00:00:00

abstract：:The polymerase chain reaction (PCR) has proved to be a sensitive and versatile method for the analysis of human and murine cytokine mRNA expression. This paper describes for the first time a reverse transcription-polymerase chain reaction (RT-PCR) at end-point for the quantification of five porcine cytokines: interfer...

journal_title：Journal of immunological methods

authors： Dufour V,Arnauld C,Lantz O,Peguillet I,Morvilliers K,Emmanuel A,Jestin A

更新日期：1999-10-29 00:00:00

abstract：:The aim of the study was to develop a novel system permitting automated analysis of multicolor immunofluorescence-staining of cells in solid tissues which would be comparable to the analytical capacity of flow cytometry. In the user friendly automated data acquisition and image processing system which was established,...

journal_title：Journal of immunological methods

authors： Steiner GE,Ecker RC,Kramer G,Stockenhuber F,Marberger MJ

更新日期：2000-04-03 00:00:00

abstract：:The binding of cells to paramagnetic polystyrene microbeads in the absence of coupling antibodies was measured. Cells from normal bone marrow, from an acute lymphoblastic leukemia (ALL) cell line or from a neuroblastoma cell line, were labeled with the fluorochrome Hoechst 33342 and incubated with microbeads by rotati...

journal_title：Journal of immunological methods

authors： Janssen WE,Rios AM

更新日期：1989-07-26 00:00:00

abstract：:In this report the use of surface-linkage to expand the potential experimental and therapeutic applications of single chain antibody (scFv) constructs is reviewed. A strategy for the generation and functional characterization of surface-linked scFvs that bind selectively to the T-cell proteins CD3epsilon, CD28, and CD...

journal_title：Journal of immunological methods

authors： Griffin MD,Holman PO,Tang Q,Ashourian N,Korthäuer U,Kranz DM,Bluestone JA

更新日期：2001-02-01 00:00:00

abstract：:Dot-immunobinding assay for anti-calmodulin (CaM), and immunoblot assay for CaM have become feasible by proper choice of a fixative, i.e., formaldehyde vapor. The protocol for these simple and convenient immunological procedures is presented. Dot-immunobinding assay could be accomplished in 3 h, and immunoblot assay i...

journal_title：Journal of immunological methods

authors： Natsukari N,Ohta H,Fujita M

更新日期：1989-12-20 00:00:00

abstract：:In the field of sexually transmitted infections (STI), the cervicovaginal explant (CVEx) model, not only provides the opportunity to study the different immunological arms present in these tissues under steady state conditions, but also their response against ex vivo infection with relevant pathogens. The methodology ...

journal_title：Journal of immunological methods

authors： Cantero J,Genescà M

更新日期：2018-09-01 00:00:00

abstract：:Mixtures of sheep erythrocytes and immune spleen cells from mice were incubated in shallow slide chambers coated with erythrocyte ghosts or anti-mouse Ig, with poly L-lysine as a coupling agent. Antigen-binding cells and erythrocytes surrounding antibody-releasing lymphocytes became bound to the reactive surfaces by i...

journal_title：Journal of immunological methods

authors： Wilson AR,Cruickshank WJ,Pugh-Humphreys RG,Jeffries AH

更新日期：1978-01-01 00:00:00

abstract：:The therapeutic use of dendritic cells (DC) in antigen-specific anti-tumor vaccines, requires sufficient numbers of functional DC, the preparation of which should comply with the code of Good Manufacturing Practice. In addition, the expression of tumor specific antigen should be possible in these DC. As a preclinical ...

journal_title：Journal of immunological methods

authors： Rouard H,Léon A,Klonjkowski B,Marquet J,Tennezé L,Plonquet A,Agrawal SG,Abastado JP,Eloit M,Farcet JP,Delfau-Larue MH

更新日期：2000-07-31 00:00:00

abstract：:Dendritic Cell (DC)-based vaccination approaches in man require a reproducible DC generation method that can be performed in conformity with GMP (Good Manufacturing Practice) guidelines and that circumvents the need for multiple blood drawings to generate DC. To this end we modified our previously described method to ...

journal_title：Journal of immunological methods

authors： Thurner B,Röder C,Dieckmann D,Heuer M,Kruse M,Glaser A,Keikavoussi P,Kämpgen E,Bender A,Schuler G

更新日期：1999-02-01 00:00:00