Abstract:
:Attempts were made to estimate peripheral T-lymphocytes in rabbits by their ability to form rosettes with sheep, human, guinea-pig, rat, mouse and chicken RBC. The reaction was studied under different conditions of pH and temperature. The test was also carried out in the presence or absence of glutaraldehyde. With sheep RBC, lymphocytes from 2 of the 5 rabbits studied formed rosettes to the extent of 1.5% in the system containing glutaraldehyde. These results however were not reproducible when studied after a week. The RBCs from other species, under similar experimental conditions, did not give rise to rosettes. The test carried out at pH 6.8 and pH 7.2 showed up to 4 and 8% rosettes respectively with rat RBCs, while RBCs of no other species showed rosettes. When rabbit lymphocytes preincubated at 37 degrees C for 1 h were used for rosette test with guinea-pig RBC, a maximum of 14.6% rosettes were obtained. Here, again the percentage of rosettes formed in the 5 animals, over a period of one month, varied widely. The reasons for the variations of the percentage of rosettes formed are discussed.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Vakharia DD,Vakharia MR,Rao SSdoi
10.1016/0022-1759(77)90165-xsubject
Has Abstractpub_date
1977-01-01 00:00:00pages
141-8issue
1-2eissn
0022-1759issn
1872-7905pii
0022-1759(77)90165-Xjournal_volume
18pub_type
杂志文章abstract::This paper describes a new and easy to handle reusable minifermenter for high-density culture of hybridoma and other cells. The culture apparatus is composed of two modules: a 40 ml disposable cell culture and antibody production chamber (the 'production module') and a 550 ml medium reservoir (the 'supply module'). Th...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)00266-y
更新日期:1995-02-13 00:00:00
abstract::The influence of antibody valency in a displacement immunoassay was investigated by comparing the whole antibody molecule with the corresponding Fab-fragment. The displacement immunoassay for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) takes advantage of the cross-reactivity of monoclonal anti-2,4-D antibodie...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(98)00211-7
更新日期:1999-03-04 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(88)90401-2
更新日期:1988-04-06 00:00:00
abstract::A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of effic...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2020.112794
更新日期:2020-08-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2006.08.010
更新日期:2006-10-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(02)00025-x
更新日期:2002-04-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(96)00097-x
更新日期:1996-10-16 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(78)90082-0
更新日期:1978-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90286-0
更新日期:1994-07-12 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(01)00560-9
更新日期:2002-03-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2016.12.002
更新日期:2017-02-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(80)90218-5
更新日期:1980-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90072-8
更新日期:1994-01-03 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90397-2
更新日期:1994-04-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(98)00065-9
更新日期:1998-06-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(87)90454-6
更新日期:1987-03-12 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2012.09.012
更新日期:2013-01-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(86)90038-4
更新日期:1986-05-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2011-09-30 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Journal of immunological methods
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更新日期:2002-05-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(87)90310-3
更新日期:1987-02-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90053-x
更新日期:1990-07-03 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2014.07.003
更新日期:2014-11-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2006.07.002
更新日期:2006-08-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00084-n
更新日期:1995-08-18 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)00249-v
更新日期:1995-01-13 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(99)00194-5
更新日期:2000-01-13 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90115-c
更新日期:1990-11-06 00:00:00