Abstract:
:A range of partial denaturation antibody pre-treatments that affect immunoassay performance have been evaluated. Monoclonal anti-ferritin antibody was either partially denatured by heat, urea or pH pre-treatment or left untreated and then passively adsorbed to polystyrene microtiter wells. The adsorption characteristics and functionality of the different surfaces produced have been evaluated by dynamic contact angle (DCA) analysis and scanning tunnelling microscopy (STM) imaging respectively. The DCA data show that the effect of partial denaturation is to change the wetting characteristics of the antibody surfaces, while, in addition, STM imaging reveals marked effects seen in the aggregation properties of the denatured antibodies.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Davies J,Dawkes AC,Haymes AG,Sunderland RF,Edwards JCdoi
10.1016/0022-1759(95)00141-vsubject
Has Abstractpub_date
1995-10-12 00:00:00pages
111-23issue
1eissn
0022-1759issn
1872-7905pii
002217599500141Vjournal_volume
186pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2011-08-31 00:00:00
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journal_title:Journal of immunological methods
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(93)90324-z
更新日期:1993-11-05 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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