Abstract:
:A simple immunoenzyme technique is described in which the enzyme beta-galactosidase of E. coli (Z) is used as a marker. The principle of the test is based on the ability of the cells to bind the antigen. Conditions of the assay using Z both as the antigen and marker are described. As a screening technique, conditions are defined at limited antigen concentrations when binding is not directly proportional to the number of cells present. Hence, drops of blood can be used directly for detection of an immune response without counting the cells. Furthermore, treatment of whole blood with ethanol was shown to (a) increase the binding capacity and (b) allow the storage of specimens for weeks without any loss of activity. With human hydatid fluid (HHF) antigens conjugated to Z as a model, it was possible to detect the immune response of rabbits injected with HHF. The method is simple, requires no sophisticated equipment and can be used in large scale epidemiological surveys.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Bahr G,Modabber FZdoi
10.1016/0022-1759(80)90268-9subject
Has Abstractpub_date
1980-01-01 00:00:00pages
205-16issue
3-4eissn
0022-1759issn
1872-7905pii
0022-1759(80)90268-9journal_volume
38pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:1992-01-21 00:00:00
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journal_title:Journal of immunological methods
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更新日期:1996-11-13 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1995-02-27 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90222-3
更新日期:1983-07-29 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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更新日期:1988-11-10 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90286-0
更新日期:1994-07-12 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1996-12-15 00:00:00
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journal_title:Journal of immunological methods
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更新日期:2004-06-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(93)90356-c
更新日期:1993-12-03 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1994-04-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90253-p
更新日期:1992-05-18 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(78)90037-6
更新日期:1978-01-01 00:00:00
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更新日期:1998-08-01 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1976-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1979-05-10 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1999-10-29 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(80)90070-8
更新日期:1980-01-01 00:00:00