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A novel radioimmunoassay for human erythropoietin using a synthetic NH2-terminal polypeptide and anti-peptide antibodies.

Abstract：

:Antibodies prepared by immunizing rabbits with a synthetic polypeptide representing the first 26 amino acids of the proposed NH2-terminal sequence of human erythropoietin cross-react with peptide, highly purified 125I-labeled erythropoietin and biologically active erythropoietin. We have now developed a rapid and convenient radioimmunoassay for the hormone using 125I-labeled peptide and anti-peptide antibodies. This peptide/anti-peptide approach completely circumvents the relative lack of availability of highly purified 125I-labeled erythropoietin and of erythropoietin for immunization purposes and makes a radioimmunoassay for the hormone universally available to investigators. This concept may be applied as a general method for designing radioimmunoassays of molecules available in severely restricted quantities.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Sytkowski AJ,Sue JM

doi

10.1016/0022-1759(84)90316-8

subject

Has Abstract

pub_date

1984-04-27 00:00:00

pages

181-6

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(84)90316-8

journal_volume

69

pub_type

杂志文章

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