Analysis of SIV-specific CTL in the rhesus macaque model of AIDS: the use of simian fibroblasts as an alternative source of target cells for chromium release assays.

abstract：:Irradiated human peripheral blood lymphoid cells undergo apoptosis and progressively exhibit typical changes in light scatter and plasma membrane integrity that can be easily tracked by flow cytometry. Using this model, we assessed the capacity of a newly developed fluorochrome, Apostain, in identifying early apoptosi...

journal_title：Journal of immunological methods

authors： Ferlini C,Kunkl A,Scambia G,Fattorossi A

更新日期：1997-06-23 00:00:00

abstract：:Two different methods have been used to study immune responses in the rat to Streptococcus pneumoniae type 3 and type 4 capsular polysaccharides (PPS). First, for simultaneous detection of the specificity and isotype of anti-PPS antibody-containing cells (ACC) in cryostat sections of lymphoid tissue, a double immunocy...

journal_title：Journal of immunological methods

authors： van den Dobbelsteen GP,van Rooijen N,Sminia T,van Rees EP

更新日期：1991-12-15 00:00:00

abstract：:Mold contamination and exposure to fungi in indoor environments has been associated with various adverse health effects but little is known about the significance of individual fungal species in the initiation or exacerbation of such effects. Using Stachybotrys chartarum as a model fungus we sought to demonstrate that...

journal_title：Journal of immunological methods

authors： Schmechel D,Simpson JP,Beezhold D,Lewis DM

更新日期：2006-02-20 00:00:00

abstract：:Near-infrared Raman spectroscopy may provide a highly sensitive, noninvasive means to identify activation status of leukocytes. The purpose of the current study was to establish Raman spectroscopic characteristics of T cell activation. Activation of the RsL.11 T cell clone in vitro with Con A resulted in specific decr...

journal_title：Journal of immunological methods

authors： Mannie MD,McConnell TJ,Xie C,Li YQ

更新日期：2005-02-01 00:00:00

abstract：:Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC...

journal_title：Journal of immunological methods

authors： Joshi L,Ponnana M,Sivangala R,Chelluri LK,Nallari P,Valluri VL,Gaddam S

更新日期：2016-05-01 00:00:00

abstract：:Little is known about the correlation between the amount of anti-A antibodies and the respective complement activating capacity. We have therefore developed a new ELISA procedure for the study of complement activation by anti-A antibodies bound to immobilized blood group substance A through the classical pathway. C1q ...

journal_title：Journal of immunological methods

authors： von Allmen E,Rieben R,Nydegger UE

更新日期：1994-05-02 00:00:00

abstract：:Peritoneal eosinophilia frequently occurs in patients undergoing peritoneal dialysis. We have devised a method for isolating large numbers of these peritoneal eosinophils from pediatric patients on continuous peritoneal dialysis. Patients were selected on the basis of previous high peritoneal eosinophil counts and had...

journal_title：Journal of immunological methods

authors： Roberts RL,Ank BJ,Salusky IB,Stiehm ER

更新日期：1990-02-09 00:00:00

abstract：:A purification method for a human urinary glycoprotein (HGP92) dissociated from Tamm Horsfall protein (THP) is described. Tamm-Horsfall protein, obtained by salt precipitations, was again precipitated in presence of monovalent ions. In these conditions, Tamm-Horsfall protein displayed a tendency to form a gel. After u...

journal_title：Journal of immunological methods

authors： Fontan E,Jusforgues-Saklani H,Briend E,Fauve RM

更新日期：1995-11-16 00:00:00

abstract：:A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery. ...

journal_title：Journal of immunological methods

authors： Series JJ,Shapiro D,Cameron I,Smith J,Fraser WD,Garden OJ,Shenkin A

更新日期：1988-04-06 00:00:00

abstract：:Human recombinant soluble 37 kDa CD23 has been expressed in insect cells and secreted into the culture medium using the IL-2 leader sequence. The 37 kDa CD23 was purified 600-fold to homogeneity by monoclonal antibody affinity chromatography and gel filtration. The pure protein is monomeric, glycosylated, depleted of ...

journal_title：Journal of immunological methods

authors： Graber P,Jansen K,Pochon S,Shields J,Aubonney N,Turcatti G,Bonnefoy JY

更新日期：1992-05-18 00:00:00

abstract：:A simple and rapid test (LIMM, short for latex immunoassay) is described for detecting Salmonella typhi endotoxin. It involves the simultaneous binding of the antigen by two types of reagent particles contained in a micro-tube: an indicator latex particle coated with a monoclonal antibody specific for the O-9 determin...

journal_title：Journal of immunological methods

authors： Lim PL

更新日期：1990-12-31 00:00:00

abstract：:In general, antigen affinity columns made with commercially prepared activated affinity supports bind antibody specific for the coupled antigen. Nonetheless, in some cases affinity purification may yield antibodies to molecules other than the molecule of interest. In this report, we demonstrate such an occurrence: an ...

journal_title：Journal of immunological methods

authors： Sweet SC,Gnegy ME,Welsh MJ

更新日期：1991-01-24 00:00:00

abstract：:Conditions were optimized for measuring the activity of myeloperoxidase (MPO) and the eosinophil peroxidase (EPO) with tetramethylbenzidine (TMB) as the substrate. Detergents caused a small increase in the measured activity of the purified enzymes and were required when isolated neutrophils or eosinophils were assayed...

journal_title：Journal of immunological methods

authors： Bozeman PM,Learn DB,Thomas EL

更新日期：1990-01-24 00:00:00

abstract：:An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange s...

journal_title：Journal of immunological methods

authors： Luk JM,Lindberg AA

更新日期：1991-03-01 00:00:00

abstract：:The fluorescence activated cell sorter (FACS) was used with an indirect membrane immunofluorescence technique to detect antibody against the Forssman antigen, a glycosphingolipid. Sheep erythrocytes, which contain Forssman antigen as a major membrane glycosphingolipid, were used as the target antigen. Detection of the...

journal_title：Journal of immunological methods

authors： Iwamori M,Mogi M,Hirano Y,Nishio M,Nakauchi H,Okumura K,Nagai Y

更新日期：1983-02-25 00:00:00

abstract：:A method for the preservation of mouse peritoneal macrophages is described. Using 5% dimethylsulphoxide with either a cooling rate of 1 degree C/min or two-step cooling with 10 min interruption at -30 degrres C allows 80% survival as judged by pinocytic activity. An important finding was that the cells must be handled...

journal_title：Journal of immunological methods

authors： Foreman S,Schorlemmer HU,Farrant J,Pegg DE,Allison AC

更新日期：1977-01-01 00:00:00

abstract：:Human tumor infiltrating lymphocytes (TIL) from metastatic melanoma of six patients were grown using a new hollow fiber bioreactor system. After inoculating 0.35-10 X 10(8) TIL into the extra-fiber space (EFS), each Cellmax bioreactor was perfused with AIM-V medium, supplemented with rIL-2. The cells subsequently expa...

journal_title：Journal of immunological methods

authors： Knazek RA,Wu YW,Aebersold PM,Rosenberg SA

更新日期：1990-02-20 00:00:00

abstract：:Phagocytosis plays a pivotal and essential role in host immune defense, both as a focal constituent of the innate immune response and a bridging element linking innate and adaptive immunity. Phagocytosis has been demonstrated to be critical in development, tissue remodeling, wound healing and resolution of inflammatio...

journal_title：Journal of immunological methods

authors： Neaga A,Lefor J,Lich KE,Liparoto SF,Xiao YQ

更新日期：2013-04-30 00:00:00

abstract：:Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was...

journal_title：Journal of immunological methods

authors： Zhao J,Chang W,Liu L,Xing X,Zhang C,Meng H,Gopinath SCB,Lakshmipriya T,Chen Y,Liu Y

更新日期：2021-02-01 00:00:00

abstract：:In the guinea pig system, there are no T lymphocyte tumors available. In addition, in this species the production of T cell lines in vitro for analysis of T cell markers or function proved to be difficult. As an alternative approach, guinea pig X mouse and guinea pig X rat T cell hybridomas were obtained by fusion of ...

journal_title：Journal of immunological methods

authors： Schäfer H,Müller B,Bader A,Schenkel J,Burger R

更新日期：1989-03-31 00:00:00

abstract：:As much as one percent of antibodies in human plasma are reported to be specific for the non-human disaccharide structure αGal. Various microbes express αGal. However, the implications of anti-αGal antibodies for the anti-microbial defenses are poorly established. With the perspective of studying the biological import...

journal_title：Journal of immunological methods

authors： Bernth-Jensen JM,Møller BK,Jensenius JC,Thiel S

更新日期：2011-10-28 00:00:00

abstract：:The techniques of flow cytometry are being increasingly used to classify lymphocytes according to their surface antigens. Accurate analyses depend on the exclusion of non-lymphocytic contaminant cells. Using a fluorescence-activated cell sorter (FACS IV), virtually complete separation of human lymphocytes from monocyt...

journal_title：Journal of immunological methods

authors： Ritchie AW,Gray RA,Micklem HS

更新日期：1983-11-11 00:00:00

abstract：:Simian immunodeficiency virus (SIV)-infection in macaques provides an important animal model for human immunodeficiency virus-1 (HIV-1) infection. The involvement of perforin (PFN), released by cytotoxic cells to mediate killing of virus-infected cells, has been difficult to assess in this experimental model due to a ...

journal_title：Journal of immunological methods

authors： Zuber B,Quigley MF,Critchfield JW,Shacklett BL,Abel K,Miller CJ,Mörner A,Paulie S,Ahlborg N,Sandberg JK

更新日期：2006-05-30 00:00:00

abstract：:The purpose of this study was to compare and contrast two enzyme immunoassay systems: the enzyme-linked immunosorbent assay (ELISA), which utilizes polystyrene microtiter plates as the adsorptive surface and the enzyme-linked immunoflow assay (ELIFA), which utilizes nitrocellulose membranes. The principal parameter un...

journal_title：Journal of immunological methods

authors： Shields MJ,Siegel JN,Clark CR,Hines KK,Potempa LA,Gewurz H,Anderson B

更新日期：1991-08-09 00:00:00

abstract：:A simple and specific enzyme-linked immunosorbent assay (ELISA) has been developed to detect circulating IgG and IgM anti-idiotypic antibodies directed against anti-HBs molecules using 96-well polyvinyl microtitre plates as the solid phase and HRPO-labelled goat anti-HBs as conjugate. Anti-idiotype reactions were obse...

journal_title：Journal of immunological methods

authors： Irshad M,Gandhi BM,Acharya SK,Joshi YK,Tandon BN

更新日期：1987-02-11 00:00:00

abstract：:The influence of size heterogeneity of IgA on its immunonephelometric (IN) assay was studied using highly purified preparations of monoclonal (MC) or polyclonal (PC) monomeric (M), dimeric (D), trimeric (T) and tetrameric (Q) IgA, as well as of secretory IgA (sIgA). Concentrations measured by optical density (OD) were...

journal_title：Journal of immunological methods

authors： Delacroix DL,Vaerman JP

更新日期：1982-01-01 00:00:00

abstract：:Immunoabsorbents for the removal of antibodies against cell membrane antigens have been prepared by a variety of techniques in common use. Cells were surface-labelled by lactoperoxidase-catalysed radioiodination, and the different immunoabsorbent preparations were compared in terms of efficiency of incorporation and s...

journal_title：Journal of immunological methods

authors： Zola H

更新日期：1978-01-01 00:00:00

abstract：:A polymorphism at position 559 in the cDNA of the FCGR3A gene encoding the FcgammaRIIIa generates two allotypes with either a valine (Val) or a phenylalanine (Phe) at amino acid position 158. This polymorphism is of major importance in immunopathology and in pharmacogenetics, especially monoclonal antibody treatments....

journal_title：Journal of immunological methods

authors： Dall'Ozzo S,Andres C,Bardos P,Watier H,Thibault G

更新日期：2003-06-01 00:00:00

abstract：:Many antigens are shared between different types of human leukocytes. In an effort to obtain new lineage-specific monoclonal antibodies, particularly antibodies to dendritic cells, we attempted to tolerize newborn mice to one type of leukocyte and then immunize the adults with another. We found that T cells, either un...

journal_title：Journal of immunological methods

authors： Yamaguchi Y,O'Doherty U,Peng M,Steinman RM

更新日期：1995-04-12 00:00:00

abstract：:A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Average values for SOD-...

journal_title：Journal of immunological methods

authors： Del Villano BC,Tischfield JA

更新日期：1979-01-01 00:00:00