The use of a hapten-Fab conjugate to sensitize target cells for antibody-dependent complement-mediated lysis and antibody-dependent cell-mediated cytotoxicity.

abstract：:A cell membrane permeabilizing treatment is described which involves the use of lysolecithin at low concentration in acidic acetate buffer and paraformaldehyde fixation. It preserved well-separated scatter cytograms of small and large lymphocytes. The accuracy of the immunochemical detection of internal antigens by fl...

journal_title：Journal of immunological methods

authors： Labalette-Houache M,Torpier G,Capron A,Dessaint JP

更新日期：1991-04-25 00:00:00

abstract：:By a rosette assay with ox red blood cells (ORBC) sensitised with rabbit IgM and IgG antibodies (EA mu and EA gamma), receptors for IgM (Fc mu-R) and IgG (Fc gamma-R) on the surface of guinea pig lymphocytes in the different lymphoid organs were demonstrated. A double rosette assay with rabbit erythrocytes (RRBC) and ...

journal_title：Journal of immunological methods

authors： Kataoka S,Itoh K,Kurane I,Kumagai K

更新日期：1982-01-01 00:00:00

abstract：:A solid-phase radioimmunometric binding assay is described utilizing 125I-labeled protein A for the detection of antibody to human melanoma associated antigens. The novel aspect of this assay is the use of chemically defined spent culture medium of melanoma cells at target antigens previously depleted of fibronectin b...

journal_title：Journal of immunological methods

authors： Morgan AC Jr,Galloway DR,Wilson BS,Reisfeld RA

更新日期：1980-01-01 00:00:00

abstract：:The immune system is characterized by high combinatorial complexity that necessitates the use of specialized computational tools for analysis of immunological data. Machine learning (ML) algorithms are used in combination with classical experimentation for the selection of vaccine targets and in computational simulati...

journal_title：Journal of immunological methods

authors： Zhang GL,Lin HH,Keskin DB,Reinherz EL,Brusic V

更新日期：2011-11-30 00:00:00

abstract：:The study of T cell receptor (TCR) genes has been hampered by their large repertoires and elusive methods for gene amplification. We have developed a new method for amplification of all human TCR genes (alpha, beta, gamma, and delta) with the ligation of a universal adaptor to the leader sequence of variable (V) regio...

journal_title：Journal of immunological methods

authors： Tsuruta Y,Iwagami S,Furue S,Teraoka H,Yoshida T,Sakata T,Suzuki R

更新日期：1993-05-05 00:00:00

abstract：:We have developed a large scale (10 g/batch) non-chromatographic method for the collection of IgM directly from plasma obtained from therapeutic plasmapheresis of patients. The technique uses sequential precipitations from ammonium sulfate and polyethylene glycol, and gives high yields of undenatured IgM antibodies. T...

journal_title：Journal of immunological methods

authors： Tatum AH

更新日期：1993-01-14 00:00:00

abstract：:A new and simple method for enzyme immunoassay of folic acid (FA) has been developed, which does not require extraction or heat denaturation of serum. FA-free serum for standards was prepared by a new immunosorbent technique as conventional methods were unsuccessful. The detection limit of the assay is 0.05 ng/ml. Int...

journal_title：Journal of immunological methods

authors： Das Sarma J,Duttagupta C,Ali E,Dhar TK

更新日期：1995-07-17 00:00:00

abstract：:Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to...

journal_title：Journal of immunological methods

authors： Jain K,Jain NK

更新日期：2015-07-01 00:00:00

abstract：:A procedure to enhance incorporation of radioactive iodine into cell-surface proteins is described. The effects of chemical modification of cells with the iodinatible haptens N-succinimidyl-3-(4-hydroxyphenyl) propionate (Bolton-Hunter reagent) and methyl-p-hydroxybenzimidate HC1 (Wood reagent) are compared. The Wood ...

journal_title：Journal of immunological methods

authors： Wall KA,Fitch FW

更新日期：1985-02-28 00:00:00

abstract：:The antigen capture capacity of antibodies covalently immobilized on injection-molded polystyrene beads was evaluated. Bromoacetyl groups on the bead surfaces rendered them reactive to protein nucleophilic groups. The bromoacetyl surface exhibited up to a ten-fold greater capacity for protein compared to unmodified po...

journal_title：Journal of immunological methods

authors： Peterman JH,Tarcha PJ,Chu VP,Butler JE

更新日期：1988-07-22 00:00:00

abstract：:The detection of site-directed anti-idiotypic antibodies is usually based on their ability to inhibit the binding of antigen to idiotype, in either solid- or fluid-phase radioimmunoassays. Passage of serum over antigen-coupled immunosorbents for the purpose of removing the idiotypes from complexes with putative anti-i...

journal_title：Journal of immunological methods

authors： Hagen M,Strejan GH

更新日期：1987-06-26 00:00:00

abstract：:The immuno-modulating properties of different adjuvant systems on the murine humoral and cellular immune response to a synthetic peptide comprising amino acid residues 9-21 of glycoprotein D of herpes simplex virus type 1 (HSV-1) were investigated. For immunization, the peptide was conjugated to ovalbumin or bovine se...

journal_title：Journal of immunological methods

authors： Geerligs HJ,Weijer WJ,Welling GW,Welling-Wester S

更新日期：1989-11-13 00:00:00

abstract：:TNF antagonists such as infliximab are effective for the treatment of several inflammatory and autoimmune diseases. Recent clinical studies have advocated the importance of measuring trough infliximab levels to guide treatment decisions. We have developed a novel assay for measuring serum free infliximab levels using ...

journal_title：Journal of immunological methods

authors： Pavlov IY,Parker RL,Lázár-Molnár E,Strathmann FG,Delgado JC

更新日期：2019-07-01 00:00:00

abstract：:Hybridoma cells producing antibody reactive with poly(A) were isolated following fusion of spleen cells from a BALB/c mouse immunized with poly(A).poly(dT) and myeloma cells. The antibody was purified from ascitic fluid by the formation of immune complexes with poly(A). The antibody reacted with poly(A) and some homop...

journal_title：Journal of immunological methods

authors： Kitagawa Y,Ishibashi K

更新日期：1993-08-26 00:00:00

abstract：:Using a solid-phase non-radioisotopic (non-RI) reverse transcriptase (RT) assay, antibodies inhibiting human immunodeficiency virus type 1 (HIV-1) RT activity (RTI antibody) were investigated for their ability to inhibit binding of RT to a template-primer and DNA polymerization. The RTI antibody inhibited the binding ...

journal_title：Journal of immunological methods

authors： Odawara F,Sano K,Otake T,Okubo S,Nakano T,Takasaki T,Ueba N,Misaki H,Nakai M

更新日期：1996-12-15 00:00:00

abstract：:We describe the setting up and validation of a reporter gene assay for type I IFN based on monkey Vero cells transfected with pMx-Luc, a plasmid carrying the luciferase gene under the control of the type I IFN inducible mouse Mx1 promoter. Vero cells were stably transfected with pMx-Luc and clone 3-143/5 was selected ...

journal_title：Journal of immunological methods

authors： Canosi U,Mascia M,Gazza L,Serlupi-Crescenzi O,Donini S,Antonetti F,Galli G

更新日期：1996-11-29 00:00:00

abstract：:A fluorimetric method using 4-methylumbelliferyl heptanoate (MUH) has been developed for detecting cell-mediated cytotoxicity and cell proliferation. The assay is based on the hydrolysis of the fluorochrome (MUH) by intracellular esterases of viable cells resulting in the production of highly fluorescent 4-methylumbel...

journal_title：Journal of immunological methods

authors： Virág L,Kerékgyártó C,Fachet J

更新日期：1995-09-25 00:00:00

abstract：:Respiratory enteric orphan virus (reovirus) has been used to study many aspects of the biology and genetics of viruses, viral infection, pathogenesis, and the immune response to virus infection. This report describes the functional activity of virus labeled with Alexa Fluor 488, a stable fluorescent dye. Matrix assist...

journal_title：Journal of immunological methods

authors： Fecek RJ,Busch R,Lin H,Pal K,Cunningham CA,Cuff CF

更新日期：2006-07-31 00:00:00

abstract：:This paper describes the evaluation and optimisation of boric acid antigen retrieval (AR) in rat joint tissue immunohistochemistry (IHC), with reference to two sample IHC targets, CD31 (PECAM-1) and Proliferating Cell Nuclear Antigen (PCNA). Sections of buffered formalin-fixed arthritic tibial/talus joints, decalcifie...

journal_title：Journal of immunological methods

authors： Wilson E,Jackson S,Cruwys S,Kerry P

更新日期：2007-04-30 00:00:00

abstract：:This communication describes an automated micro-adherence inhibition assay. Tumor-specific immunity was demonstrated with B16 melanoma and MCA-38 colon adenocarcinoma, both of which are syngeneic to the same strain of mouse (C57B16/J). Abrogation of the leukocyte adherence inhibition (LAI) response of sensitized leuko...

journal_title：Journal of immunological methods

authors： Leveson SH,Howell JH,Holyoke ED,Goldrosen MH

更新日期：1977-01-01 00:00:00

abstract：:In this study the exposure period of the lymphocyte-myeloma cell mixture to the fusogen was evaluated for its influence upon the yield of total hybridoma colonies and those which secreted monoclonal antibodies. Sp2/0 and FOX-NY myeloma cells were fused for varying periods with murine splenic lymphocytes immunized with...

journal_title：Journal of immunological methods

authors： Lane RD

更新日期：1985-08-02 00:00:00

abstract：:The level of CD4(+) T-lymphocytes represents a useful marker with which to monitor the progression of HIV infection. Sex and geographical differences in the reference values of lymphocyte subsets have been reported. We have compared two flow cytometric methods (MultiSET and SimulSET) for the quantification of lymphocy...

journal_title：Journal of immunological methods

authors： Urassa WK,Mbena EM,Swai AB,Gaines H,Mhalu FS,Biberfeld G

更新日期：2003-06-01 00:00:00

abstract：:Dengue fever is caused by the dengue virus (DENV), and DENV1 is the prevalent epidemic serotype in south China. A new lateral flow assay (LFA) based on a near-infrared (NIR) fluorescent dye was developed to detect anti-DENV1 IgG antibodies. DyLight-800 was used as the marker conjugated to goat anti-human IgG antibodie...

journal_title：Journal of immunological methods

authors： Chen L,Wang H,Guo T,Xiao C,Liu L,Zhang X,Liu B,Li P,Liu A,Li B,Li B,Mao Y

更新日期：2018-05-01 00:00:00

abstract：:A microtechnique to study lymphocyte stimulation in solid cultures by autoradiography is described. Using this technique at a concentration of 2 x 10(6) lymphocytes/chamber, it was found that the percentage of proliferating cells was 8.4% with anti-lymphocyte serum, 3.9% with calcium ionophore A23187, 2.5% with phytoh...

journal_title：Journal of immunological methods

authors： Kondracki E

更新日期：1979-01-01 00:00:00

abstract：:A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well E...

journal_title：Journal of immunological methods

authors： Kobayashi N,Sugita H,Terada E,Ghoda A,Okudaira H,Ogita T,Miyamoto T

更新日期：1984-10-26 00:00:00

abstract：:The production and characterisation of 17 monoclonal antibodies to human parathyroid hormone-related protein (PTH-rP) 1-34 is described. Five of the antibodies were shown to be of high avidity (Ka 4 X 10(10)-1.9 X 10(11) L/M) and able to detect 15-100 pg PTH-rP 1-34 per tube by RIA. None cross-reacted with PTH 1-34, a...

journal_title：Journal of immunological methods

authors： Ratcliffe WA,Hughes S,Gilligan MG,Heath DA,Ratcliffe JG

更新日期：1990-02-20 00:00:00

abstract：:Multiplex bead-based flow cytometry is an attractive way for simultaneous, rapid and cost-effective analysis of multiple analytes in a single sample. Previously, we developed various bead-based assays using non-magnetic beads coated with Staphylococcus aureus and Streptococcus pneumoniae antigens for the detection of ...

journal_title：Journal of immunological methods

authors： Hansenová Maňásková S,van Belkum A,Endtz HP,Bikker FJ,Veerman EC,van Wamel WJ

更新日期：2016-09-01 00:00:00

abstract：:A sensitive method for staining proteins after transfer from polyacrylamide gels to nitrocellulose paper is described. Transferred proteins are first derivatized by reaction of the nitrocellulose replica with sulfosuccinimidobiotin and are then reacted sequentially with streptavidin, rabbit anti-streptavidin, and hors...

journal_title：Journal of immunological methods

authors： LaRochelle WJ,Froehner SC

更新日期：1986-08-21 00:00:00

abstract：:The guinea pig has a storied history as a model in the study of infectious disease and immunology. Because of reproducibility of data and availability of various reagents, inbred mice have since supplanted the guinea pig as the animal model-of-choice in these fields. However, several clinically-significant microorgani...

journal_title：Journal of immunological methods

authors： Yu KO,Porcelli SA,Shuman HA

更新日期：2013-03-29 00:00:00

abstract：:Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the ...

journal_title：Journal of immunological methods

authors： van der Velden VHJ,Flores-Montero J,Perez-Andres M,Martin-Ayuso M,Crespo O,Blanco E,Kalina T,Philippé J,Bonroy C,de Bie M,Te Marvelde J,Teodosio C,Corral Mateos A,Kanderová V,van der Burg M,Van Hoof D,van Dongen JJM,Orf

更新日期：2019-12-01 00:00:00