Abstract:
:By a rosette assay with ox red blood cells (ORBC) sensitised with rabbit IgM and IgG antibodies (EA mu and EA gamma), receptors for IgM (Fc mu-R) and IgG (Fc gamma-R) on the surface of guinea pig lymphocytes in the different lymphoid organs were demonstrated. A double rosette assay with rabbit erythrocytes (RRBC) and EA mu or EA gamma for detection of T cells with Fc mu-R (T mu) or Fc gamma-R (T gamma) was also developed. Fc mu-R was detected on 40.1%, 48.7%, 35.0% and less than 1% of peripheral blood, lymph node, spleen and thymus cells respectively. By a double rosette assay with RRBC and EA mu, approximately 30% of peripheral blood, lymph node and spleen T cells were identified as T mu cells. T gamma cells as revealed by a double rosette of RRBC and EA gamma were found to be 3% and 5% respectively of peripheral blood and lymph node T cells, whereas much higher numbers of T cells in the spleen (32.7) were identified as T gamma cells. This double rosette assay of T mu and T gamma cells in the peripheral blood of guinea pigs which had received prednisolone showed that the proportion of T mu cells decreased early after drug administration and that T gamma cells increased in number. Thus the methods provide a useful means of detecting T mu and T gamma cells in guinea pigs. This assay system for T mu and T gamma cells in guinea pigs should be useful for studies of their variation or functional significance in the immune response.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Kataoka S,Itoh K,Kurane I,Kumagai Kdoi
10.1016/0022-1759(82)90385-4subject
Has Abstractpub_date
1982-01-01 00:00:00pages
89-100issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(82)90385-4journal_volume
51pub_type
杂志文章abstract::A method is described for immunizing mice with a protein-cellulose complex obtained by covalent coupling of antigenic protein molecules to suspended cellulose particles. Primary immunization of the animals with the complex led to a pronounced immune response persisting for 20-30 days. Subsequent administration of the ...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2013-01-31 00:00:00
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journal_title:Journal of immunological methods
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更新日期:1980-01-01 00:00:00
abstract::A simple and sensitive method for immunoblotting of proteins after separation in agarose gels is described. It involves transfer of proteins onto nitrocellulose paper simply by diffusion through pressure, a transfer which only takes about 10 min. By this method we have demonstrated the existence of multiple molecular ...
journal_title:Journal of immunological methods
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更新日期:1985-11-28 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1996-09-09 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1987-10-23 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(78)90011-x
更新日期:1978-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90208-d
更新日期:1990-04-17 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90428-3
更新日期:1983-10-14 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(93)90168-7
更新日期:1993-02-26 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2006.01.003
更新日期:2006-03-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2010.02.003
更新日期:2010-04-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90208-b
更新日期:1992-09-18 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90251-x
更新日期:1983-08-26 00:00:00
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journal_title:Journal of immunological methods
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更新日期:1991-07-26 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
abstract::The Raji cell assay for circulating immune complexes (CIC) is frequently the method of choice when the detection of large, complement fixing complexes is desired. We have developed an enzyme-linked immunosorbent assay modification (Raji-ELISA) of the Raji cell technique which is easy to perform, uses commercially avai...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90046-7
更新日期:1983-01-01 00:00:00
abstract::Plasma cells (PCs) secrete antibodies and play an essential role in protective immunity, but also in pathogenesis of antibody-mediated diseases. Physiologically, PCs mainly reside within bone marrow and spleen. In autoimmune diseases such as systemic lupus erythematosus (SLE) autoantibody-producing PCs can also be fou...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2019.06.019
更新日期:2019-11-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(88)90401-2
更新日期:1988-04-06 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(01)00560-9
更新日期:2002-03-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(00)00214-3
更新日期:2000-07-31 00:00:00
abstract::Fungi represent emerging infectious threats to human populations worldwide. Mice and other laboratory animals have proved invaluable in modeling clinical syndromes associated with superficial and life-threatening invasive mycoses. This review outlines salient features of common vertebrate animal model systems to study...
journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
doi:10.1016/j.jim.2014.03.022
更新日期:2014-08-01 00:00:00
abstract::The sandwich solid phase radioimmunoassay procedure has been adapted to the serological diagnosis of tuberculosis by the use of disposable plastic haemagglutination trays in which the wells were sensitized with soluble mycobacterial antigen and the subsequent uptake of globulin from test sera detected with radioiodina...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(75)90069-1
更新日期:1975-01-01 00:00:00