Leukocyte adherence inhibition: an automated microassay demonstrating specific antigen recognition and blocking activity in two murine tumor systems.

abstract：:Using the advantages of phenol red, a signal enhancer, and bovine serum albumin (BSA), a stabilizer of horseradish peroxidase (HRP), added in HRP enzyme reaction of Amplex Red and H2O2, highly sensitive 1,1'-oxalyldiimidazole chemiluminescence enzyme immunoassay (ODI-CLEIA) was developed to rapidly quantify trace leve...

journal_title：Journal of immunological methods

authors： Kim SS,Lee Y,Shin HS,Lee JH

更新日期：2019-08-01 00:00:00

abstract：:Lymphocyte subsets from human blood obtained by different procedures were analyzed for cytotoxic potential and phenotypic characteristics. Nylon wool column passed lymphocytes were fractionated on the basis of: (1) E and Fc gamma receptor expression, (2) cell density and Fc gamma receptor expression, (3) Fc gamma rece...

journal_title：Journal of immunological methods

authors： Masucci G,Masucci MG,Bejarano MT,Klein E

更新日期：1983-09-30 00:00:00

abstract：:Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach tha...

journal_title：Journal of immunological methods

authors： Dzutsev AK,Belyakov IM,Isakov DV,Gagnon SJ,Margulies DH,Berzofsky JA

更新日期：2008-04-20 00:00:00

abstract：:The performance of immunoassays for the detection of autoantibodies is of critical importance to the diagnosis and assessment of patients with systemic lupus erythematosus (SLE). Our objective was to compare 3 multiplexed assays for measurement of multiple autoantibodies and their association with global disease activ...

journal_title：Journal of immunological methods

authors： Hanly JG,Su L,Farewell V,Fritzler MJ

更新日期：2010-06-30 00:00:00

abstract：:Dendritic cells are a specialized but trace population of antigen presenting cells that always have been enriched by multi-step procedures over a period of 1 or more days in tissue culture. Here we describe the isolation of dendritic cells from fresh mouse spleen suspensions using the FACS and a monoclonal antibody, N...

journal_title：Journal of immunological methods

authors： Crowley MT,Inaba K,Witmer-Pack MD,Gezelter S,Steinman RM

更新日期：1990-10-04 00:00:00

abstract：:The purpose of this study was to compare and contrast two enzyme immunoassay systems: the enzyme-linked immunosorbent assay (ELISA), which utilizes polystyrene microtiter plates as the adsorptive surface and the enzyme-linked immunoflow assay (ELIFA), which utilizes nitrocellulose membranes. The principal parameter un...

journal_title：Journal of immunological methods

authors： Shields MJ,Siegel JN,Clark CR,Hines KK,Potempa LA,Gewurz H,Anderson B

更新日期：1991-08-09 00:00:00

abstract：:No standard procedure is available for the purification of human monoclonal antibodies for human i.v. administration. Here we describe the procedure developed for pilot scale purification of the human IgM monoclonal antibody COU-1 directed against a cancer-associated antigen. The hybridoma cells were grown in protein-...

journal_title：Journal of immunological methods

authors： Tornøe I,Titlestad IL,Kejling K,Erb K,Ditzel HJ,Jensenius JC

更新日期：1997-06-23 00:00:00

abstract：:We describe here a spot test to detect antibodies to non-erythroid cell surface antigens, using ethidium bromide staining to visualize areas of target cell lysis. This assay allows rapid screening of large numbers of samples of culture supernatants, antisera, or ascitic fluids. Antibody titers determined by this spot ...

journal_title：Journal of immunological methods

authors： Cohn ML,Golding H,Rittenberg MB

更新日期：1982-01-01 00:00:00

abstract：:Simian immunodeficiency virus (SIV)-infection in macaques provides an important animal model for human immunodeficiency virus-1 (HIV-1) infection. The involvement of perforin (PFN), released by cytotoxic cells to mediate killing of virus-infected cells, has been difficult to assess in this experimental model due to a ...

journal_title：Journal of immunological methods

authors： Zuber B,Quigley MF,Critchfield JW,Shacklett BL,Abel K,Miller CJ,Mörner A,Paulie S,Ahlborg N,Sandberg JK

更新日期：2006-05-30 00:00:00

abstract：:This report describes the use of biotin-labeled antibodies in sensitive enzyme immunoassay systems for the measurement of bacterial antigens. Biotinylated immunoglobulins could be reproducibly formulated using N-hydroxysuccinimide biotin ester to link biotin to the immunoglobulin. Binding of the biotinylated antibody ...

journal_title：Journal of immunological methods

authors： Yolken RH,Leister FJ,Whitcomb LS,Santosham M

更新日期：1983-02-11 00:00:00

abstract：:A new and simple method for quantitation of IgM of low molecular weight in sera by radial immunodiffusion in 7% agar gel is described. The test is easy to perform and permits exact determination of low molecular weight IgM concentration down to very small quantities (1 mg%). Results obtained with the method described ...

journal_title：Journal of immunological methods

authors： Romero O,Yantorno C,Romero-Piffiguer M

更新日期：1978-01-01 00:00:00

abstract：:The short-term kinetics of T cell-mediated cytotoxicity was investigated using a cloning inhibition assay. Murine cytotoxic thymus-derived lymphocytes (CTL) generated in vitro in mixed leukocyte cultures (MLC), were incubated for various periods of time at 37 degrees C with allogeneic mastocytoma target cells. The mix...

journal_title：Journal of immunological methods

authors： Lees RK,MacDonald HR,Sinclair NR

更新日期：1977-01-01 00:00:00

abstract：:The present article reports a multiparametric cytofluorimetric analysis of apoptosis in murine thymocytes and human PBMC from healthy donors or HIV-infected patients. We have evaluated four previously described cytofluorimetric methods of apoptosis quantification, each of them detecting distinct cellular alterations o...

journal_title：Journal of immunological methods

authors： Lecoeur H,Gougeon ML

更新日期：1996-10-30 00:00:00

abstract：:A technique for detecting the nitroblue tetrazolium-reducing activity of human polymorphonuclear leukocyte enzymes separated on polyacrylamide gels is reported. Slices of gel are incubated in buffer containing nitroblue tetrazolium and either NADPH or NADH. Enzymes capable of reducing nitroblue tetrazolium form a dark...

journal_title：Journal of immunological methods

authors： Phillips WA,Shelton MJ,Hosking CS

更新日期：1982-10-29 00:00:00

abstract：:Factors influencing the performance of a dipstick competitive particle capture immunoassay (PCI) for the steroid oestrone sulphate (OS) were investigated. Appropriate 'blocking' of the nitrocellulose dipstick membrane was necessary for the upward flow of microsphere particles. Traditional protein blocking agents inclu...

journal_title：Journal of immunological methods

authors： Henderson K,Stewart J

更新日期：2002-12-01 00:00:00

abstract：:Removal and/or neutralization of preformed anti-pig antibodies in non-human primate blood have been shown to prevent the hyperacute rejection of transplanted pig organs. The purpose of this study was to establish a suitable in vitro method that would allow for screening and comparison of various agents and methods pot...

journal_title：Journal of immunological methods

authors： Neethling FA,Koscec M,Oriol R,Cooper DK,Koren E

更新日期：1999-01-01 00:00:00

abstract：:Studies describing the induction of apoptosis for CD4 mAbs do not delineate between epitope-dependent and Fc-driven epitope cross-linking induced cell death. Keliximab and clenoliximab are two CD4 mAbs that differ only in their heavy chain isotypes, being an IgG1 and a modified IgG4, respectively. These antibodies sup...

journal_title：Journal of immunological methods

authors： Fishman-Lobell J,Tsui P,Reddy M,DiPrinzio R,Eichman C,Sweet RW,Truneh A

更新日期：2001-11-01 00:00:00

abstract：:A sensitive time-resolved fluoroimmunoassay of nerve growth factor (NGF) has been developed. The method is based on the unique property of the lanthanides for delayed fluorescence, which reduces substantially the endogenous fluorescence of biological substances, because the excitation of the sample and detection of th...

journal_title：Journal of immunological methods

authors： Arumäe U,Neuman T,Sinijärv R,Saarma M

更新日期：1989-08-15 00:00:00

abstract：:Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera ven...

journal_title：Journal of immunological methods

authors： Asseman C,Pancré V,Delanoye A,Capron A,Auriault C

更新日期：1994-07-12 00:00:00

abstract：:A method for quantitative RT-PCR using ELISA detection was developed and applied to the quantitation of IL-1 beta mRNA in clinical samples. To compensate for the 'tube effect' of RT-PCR, a synthetic RNA, pRSET RNA, was added to sample solutions as an internal standard and co-amplified with IL-1 beta mRNA. Sense primer...

journal_title：Journal of immunological methods

authors： Tsuruta H,Matsui S,Oka K,Namba T,Shinngu M,Nakamura M

更新日期：1995-03-27 00:00:00

abstract：:In the production of polyclonal antibody, a purification step is necessary which is often done by affinity chromatography. We present a biosensor system based on reflectometric interference spectroscopy (RIfS) to monitor the quantity and quality in terms of affinity and kinetic constants of the antibody during this pr...

journal_title：Journal of immunological methods

authors： Proll G,Kumpf M,Mehlmann M,Tschmelak J,Griffith H,Abuknesha R,Gauglitz G

更新日期：2004-09-01 00:00:00

abstract：:Conditions for desorbing a DNP carrier from its immunoadsorbent were studied in various hydro-organic media at different pHs and temperatures, including the sub-zero range. It appeared that DNP--anti-DNP interaction is the result of a balance between various bonds and the best result (95% yield) was obtained in hydro-...

journal_title：Journal of immunological methods

authors： Andersson K,Benyamin Y,Douzou P,Balny C

更新日期：1978-01-01 00:00:00

abstract：:Immunological techniques have been used to identify and quantitate rabbit antibody light chains in the urine of rabbits. The influence of these light chains on the radioallergosorbent test (RAST) used for the diagnosis of laboratory animal allergy to rabbit urinary protein allergens is described. The presence of IgG a...

journal_title：Journal of immunological methods

authors： Edwards RG,Lee D,Dewdney JM

更新日期：1983-06-24 00:00:00

abstract：OBJECTIVE:Interleukin-13 (IL-13) has been difficult to quantify within human serum due to low abundance. Available assays have not been sensitive enough to detect IL-13 at the femtogram level. Thus, there are inconsistencies within the published literature as to IL-13 concentrations in normal or disease populations. To...

journal_title：Journal of immunological methods

authors： Gaye B,Sikkema D,Lee TN

更新日期：2015-11-01 00:00:00

abstract：:The amino-acid sequence of the very high-affinity anti-angiotensin II monoclonal antibody 4D8 was predicted from the nucleotide sequence of the heavy and light chain variable genes. The single-chain variable fragment (scFv) was constructed and expressed in Escherichia coli as a soluble protein and at the surface of th...

journal_title：Journal of immunological methods

authors： Cohen P,Laune D,Teulon I,Combes T,Pugnière M,Badouaille G,Granier C,Mani JC,Simon D

更新日期：2001-08-01 00:00:00

abstract：:One major obstacle in antibody production is the lack of highly purified immunogen. In this study, we describe an alternative strategy to circumvent this problem. A nucleotide sequence encoding a full-length of human survivin was cloned into pAK400cb. After transforming into an E. coli Origami B strain, survivin-bioti...

journal_title：Journal of immunological methods

authors： Tayapiwatana C,Chotpadiwetkul R,Kasinrerk W

更新日期：2006-12-20 00:00:00

abstract：:Mast cells are important effector cells of allergy and are involved in the pathology of many other diseases. Measurement of β-hexosaminidase activity, the most commonly used method for evaluation of murine mast cell activity, requires a large number of cells and thus is of limited utility for studying mast cells in mo...

journal_title：Journal of immunological methods

authors： Larson D,Mitre E

更新日期：2012-05-31 00:00:00

abstract：:Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation ...

journal_title：Journal of immunological methods

authors： Miyakawa S,Okui T,Shiraishi T,Yoshihara T,Hirayama M,Satomi Y,Hamada T,Nishida M,Akimoto C,Sato S

更新日期：2020-05-01 00:00:00

abstract：:We have developed a microarray (DotScan) that enables rapid immunophenotyping and classification of leukaemias and lymphomas by measuring the capture of cells by immobilized dots of 82 CD antibodies [Belov, L., de la Vega, O., dos Remedios, C.G., Mulligan, S.P., 2001. Immunophenotyping of leukemia using a cluster of d...

journal_title：Journal of immunological methods

authors： Belov L,Huang P,Chrisp JS,Mulligan SP,Christopherson RI

更新日期：2005-10-20 00:00:00

abstract：:An assay has been developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis is quantitated spectrophotometrically (QHS). Immune mouse spleen cells, SRBC and guinea pig complement were incubated at 37 degrees C for 1 h in buffer, centrifuged, and the supernatant solutions read...

journal_title：Journal of immunological methods

authors： Simpson MA,Gozzo JJ

更新日期：1978-01-01 00:00:00