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Sensitive time-resolved fluoroimmunoassay of nerve growth factor and the disappearance of nerve growth factor from rat pheochromocytoma PC12 cell culture medium.

Abstract：

:A sensitive time-resolved fluoroimmunoassay of nerve growth factor (NGF) has been developed. The method is based on the unique property of the lanthanides for delayed fluorescence, which reduces substantially the endogenous fluorescence of biological substances, because the excitation of the sample and detection of the fluorescence signal are separated in time and in wavelength. Using the europium-conjugated antibodies to the NGF from Vipera lebetina (snake) venom and to the beta NGF from mouse submandibular gland in a solid-phase quantitative two-site fluoroimmunoassay, we obtained a maximal sensitivity of 10 pg/ml (0.38 pM)for mouse NGF and 40 pg/ml (1.2 pM) for snake NGF. Using this method, we investigated the disappearance of NGF from rat pheochromocytoma PC12 cell culture medium. Mouse beta NGF (5-10 ng/ml) disappeared completely after 12 h of incubation, whereas snake NGF was not substantially internalized even after 48 h.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Arumäe U,Neuman T,Sinijärv R,Saarma M

doi

10.1016/0022-1759(89)90334-7

subject

Has Abstract

pub_date

1989-08-15 00:00:00

pages

59-65

issue

1

eissn

0022-1759

issn

1872-7905

pii

0022-1759(89)90334-7

journal_volume

122

pub_type

杂志文章

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