Abstract:
:In the production of polyclonal antibody, a purification step is necessary which is often done by affinity chromatography. We present a biosensor system based on reflectometric interference spectroscopy (RIfS) to monitor the quantity and quality in terms of affinity and kinetic constants of the antibody during this procedure. Biosensors are rapid compared with ELISA, which is done in practice and can work fully automated. They provide additional information about the active antibody to protein concentration ratio and the affinity of the antibody. We show how to determine these values very accurate. In addition, we describe a new rapid method to monitor the affinity chromatography in process. This gives the possibility to select antibody fractions with best properties in respect to the application.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Proll G,Kumpf M,Mehlmann M,Tschmelak J,Griffith H,Abuknesha R,Gauglitz Gdoi
10.1016/j.jim.2004.05.008keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
35-42issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175904001929journal_volume
292pub_type
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