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Application of the MAILA technique to the study of human anti-HLA monoclonal antibody specificity.

Abstract：

:The monoclonal antibody-specific immobilization of lymphocyte antigens (MAILA) assay was developed to detect antibodies present in human alloantisera against antigens of different major histocompatibility complex loci, particularly of class II specificity. The MAILA assay has been used in our laboratory to the determination of the type of HLA molecule recognized by human monoclonal antibodies 91C2 (anti-A2 + 28), 34F11 (anti-DQ1), and 2A2 (anti-DQ1 + 4 + short DQ7), using well characterized monomorphic as well as polymorphic murine monoclonals for the specific immobilization of HLA molecules. Results obtained show that the MAILA assay is also a valuable tool for the determination of specific human MHC locus products recognized by human monoclonal antibodies.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Sánchez B,Melero J,García-Lozano JR,Yélamos J,Magariño R,Robledo MM,Dessi V,Siervo S,Núñez-Roldán A

doi

10.1016/0022-1759(93)90094-n

subject

Has Abstract

pub_date

1993-01-04 00:00:00

pages

253-8

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(93)90094-N

journal_volume

157

pub_type

杂志文章

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