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A sensitive proliferation assay to determine the specific T cell response against HLA-A2.1-binding peptides.

Abstract：

:A proliferation assay to detect a specific T cell response against HLA class I-binding peptides is reported. To establish the specificity and sensitivity of the assay we used a synthetic peptide derived from the CMV glycoprotein B containing the HLA-A2.1 ligand motif. Lymphocyte proliferation was measured following culture of PBMC in the presence or absence of peptide by BrdU uptake. Differing culture conditions were compared (cell number, time in culture, peptide concentration, +/- IL-2). A peptide-specific response was detected in 11 of 23 HLA-A2-positive and CMV IgG-positive donors (47.8%), and in 4 of 36 HLA-A2-positive and CMV IgG-negative donors (11.1%, p = 0.019) tested under optimized conditions. None of 22 HLA-A2-negative individuals tested showed a peptide-specific response and the reproducibility was high. Peptide-specific IFN-gamma-secreting T cells could be demonstrated in responding donors with the ELISPOT assay. This proliferation assay may be suitable for monitoring induction of a specific T cell response against known HLA class I-binding peptides following vaccination with tumor or viral antigens.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Mayer S,Scheibenbogen C,Lee KH,Keilholz W,Stevanovic S,Rammensee HG,Keilholz U

doi

10.1016/0022-1759(96)00124-x

subject

Has Abstract

pub_date

1996-10-16 00:00:00

pages

131-7

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(96)00124-X

journal_volume

197

pub_type

杂志文章

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