当前位置： SCI文献检索 > JOURNAL OF IMMUNOLOGICAL METHODS期刊下所有文献 > Influence of antibody valency in a displacement immunoassay for the quantitation of 2,4-dichlorophenoxyacetic acid.

Influence of antibody valency in a displacement immunoassay for the quantitation of 2,4-dichlorophenoxyacetic acid.

Abstract：

:The influence of antibody valency in a displacement immunoassay was investigated by comparing the whole antibody molecule with the corresponding Fab-fragment. The displacement immunoassay for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) takes advantage of the cross-reactivity of monoclonal anti-2,4-D antibodies and the Fab-fragments toward immobilized 2-methyl-4-chlorophenoxyacetic acid (MCPA). Due to the low affinity of the antibodies toward MCPA (cross-reactivity of approximately 30%), the addition of 2,4-D resulted in a displacement of the antibodies or the fragments. The detection limits obtained with whole anti-2,4-D antibodies and Fab-fragments were 0.1 microg/l and 0.01 microg/l 2,4-D, respectively. The whole antibodies and the Fab-fragments show similarities, such as the cross-reactivity toward MCPA (26% and 33%), and some characteristics of the calibration curve, for example the large detection range and the sensitivity. In contrast to the bivalent antibodies, however, increasing the hapten/protein ratios of the immobilized MCPA-BSA conjugates did not affect the detection limit when using the Fab-fragments. Moreover, kinetic experiments reveal a faster displacement reaction with the Fab-fragments. A disadvantage of using the Fab-fragments is the generation of lower absorbance values in the ELISA.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Gerdes M,Meusel M,Spener F

doi

10.1016/s0022-1759(98)00211-7

keywords:

subject

Has Abstract

pub_date

1999-03-04 00:00:00

pages

217-26

issue

2

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(98)00211-7

journal_volume

223

pub_type

杂志文章

相关文献

JOURNAL OF IMMUNOLOGICAL METHODS文献大全
  • Phosphatidylinositol hydrolysis in freshly isolated human T lymphocytes.

    abstract：:Antigen receptor-mediated activation of T and B lymphocytes results in activation of phospholipase C-gamma isozymes with subsequent hydrolysis of membrane inositol phospholipids. As a method of screening autoimmune or immunodeficient patients for early receptor signaling defects, we have developed a rapid technique fo...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(95)00135-w

    authors： Berney SM,Atkinson TP

    更新日期：1995-10-12 00:00:00

  • The separation of the granulocytes from different rat strains. A comparative study.

    abstract：:Different Percoll density gradients were used to purify granulocytes from Long-Evans, Fischer, Sprague-Dawley, and Fischer/Long-Evans hybrid rats. Three different discontinuous gradient types were developed which permitted the separation of polymorphonuclear leukocytes (PMNLs) from the peripheral blood of the differen...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(94)90093-0

    authors： Szücs S,Varga C,Ember I,Kertai P

    更新日期：1994-01-03 00:00:00

  • A simple and sensitive method to study effects mediated by soluble lymphokines as demonstrated by the interaction of CD4+ and CD8+ cell subsets during T cell activation.

    abstract：:A method is described for the study of lymphokine-mediated cellular interactions using triple wells, which permits co-culture of cell subpopulations without direct physical contact. The triple wells are constructed by slitting the walls to half height between three adjacent wells of a 96-well microtiter plate. The cel...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(94)90279-8

    authors： Karawajew L,Micheel B,Jung G,Wolf H,Behrsing O

    更新日期：1994-07-12 00:00:00

  • Neutrophil CD64 expression as an important diagnostic marker of infection and sepsis in hospital patients.

    abstract：INTRODUCTION:Infection and sepsis are major health problems. Therefore, the need for improved diagnostic indicators, as well as for better therapeutic monitors in the treatment of infection, remains, since the current diagnostic tools have low specificity and passed through minimal changes in the last two decades. OBJ...

    journal_title：Journal of immunological methods

    pub_type： 信件

    doi：10.1016/j.jim.2014.07.011

    authors： Farias MG,de Lucena NP,Dal Bó S,de Castro SM

    更新日期：2014-12-01 00:00:00

  • An efficient protocol for in vivo labeling of proliferating epithelial cells.

    abstract：:The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence re...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2018.03.015

    authors： Michel C,Küchler R,Martin I,Kyewski B,Pinto S

    更新日期：2018-06-01 00:00:00

  • Sandwich immunoassay of small molecules. I. Investigation with testosterone as model hapten.

    abstract：:A new strategy has been developed for enzyme immunoassay of small molecules. The method is based on the formation and estimation of the complex Ab.H-H.Ab* where Ab and Ab* are immobilised and labeled antibodies respectively and H-H is a synthetic bis-analogue of the hapten H. The amount of the complex formed is invers...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(12)80005-6

    authors： Ali E,Sengupta J,Dhar TK

    更新日期：1992-03-04 00:00:00

  • A rapid rosette technique for quantitation and separation of mononuclear cell subsets using monoclonal antibodies.

    abstract：:We present a rapid and simple method for simultaneous quantitation and separation of mononuclear cell (MNC) subsets. When lymphoid cells are sensitized with monoclonal antibodies of the OK and Leu series, they rapidly form rosettes with ox erythrocytes (ORBC) coated with affinity-purified rabbit IgG against mouse IgG....

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(82)90033-3

    authors： Egeland T,Lea T

    更新日期：1982-12-17 00:00:00

  • Cryopreservation of mouse peritoneal macrophages.

    abstract：:A method for the preservation of mouse peritoneal macrophages is described. Using 5% dimethylsulphoxide with either a cooling rate of 1 degree C/min or two-step cooling with 10 min interruption at -30 degrres C allows 80% survival as judged by pinocytic activity. An important finding was that the cells must be handled...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(77)90139-9

    authors： Foreman S,Schorlemmer HU,Farrant J,Pegg DE,Allison AC

    更新日期：1977-01-01 00:00:00

  • A simplified procedure for studies of intestinal immunity in rabbits.

    abstract：:As interest in the development of oral vaccines continues to rise, alternative animal models for studies of mucosal immunity are needed. The present study examines a simplified procedure for delivering antigen to rabbit Peyer's patches via an indwelling cannula. The cannula was placed 3-4 cm proximal to the Peyer's pa...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(96)00055-5

    authors： McAleer FT,Silbart LK,Van Kruiningen HJ,Koudelka J,Tobias A

    更新日期：1996-07-17 00:00:00

  • An enzyme linked immunosorbent assay for leucocyte and platelet antibodies.

    abstract：:An enzyme linked immunosorbent assay (ELISA) method for the detection of antibodies to platelets and leucocytes is presented. The method can be used for large numbers of samples. The method is objective when photometers are used. Approximately 50% of all cases of possible autoimmune thrombocytopenic purpura (A.T.P.) a...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(81)90116-2

    authors： Doughty R,James V,Magee J

    更新日期：1981-01-01 00:00:00

  • Ensemble approaches for improving HLA class I-peptide binding prediction.

    abstract：:Accurately predicting peptides binding to major histocompatibility complex (MHC) I molecules is of great importance to immunologists for elucidating the underlying mechanism of immune recognition and facilitating the design of peptide-based vaccine. Various computational methods have been developed for MHC I-peptide b...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2010.09.007

    authors： Hu X,Mamitsuka H,Zhu S

    更新日期：2011-11-30 00:00:00

  • Fluorescent proteins: powerful tools in phagocyte biology.

    abstract：:Phagocyte functions such as chemotaxis and phagocytosis involve the rapid and transient development of cellular polarity. Study of this highly complex spatial and temporal cellular remodelling has been limited by the static nature of immunofluorescence and immunogold microscopy and because biochemical techniques are n...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章,评审

    doi：10.1016/s0022-1759(99)00155-6

    authors： Bajno L,Grinstein S

    更新日期：1999-12-17 00:00:00

  • Assessment of determinants of optimum performance of the CAST-2000 ELISA procedure.

    abstract：:The Cellular Antigen Stimulation Test (CAST), a recent innovation in the laboratory detection of allergen sensitivity, is a functional assay based on the release of sulphidoleukotrienes (LTs) from allergen-activated circulating basophils. The current study was undertaken to establish optimum determinants of this proce...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2003.12.014

    authors： Van Rooyen C,Anderson R

    更新日期：2004-05-01 00:00:00

  • The complex between urokinase plasminogen activator and its type-1 inhibitor in breast cancer extracts quantitated by ELISA.

    abstract：:ELISAs for urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) have shown that tumor levels of these molecules are prognostic parameters in breast cancer as well as other types of cancer. These ELISAs measure the total amount of the given component, including preforms, active, inac...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(97)00008-2

    authors： Pedersen AN,Høyer-Hansen G,Brünner N,Clark GM,Larsen B,Poulsen HS,Danø K,Stephens RW

    更新日期：1997-04-11 00:00:00

  • TNF-alpha detection using a flow cytometric assay to determine cellular responses to anthrax vaccine.

    abstract：:This study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax antigen. TNF-alpha and IFN-gamma production from memory CD4...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2003.07.012

    authors： Shinn AH,Bravo NC,Maecker HT,Smith JW

    更新日期：2003-11-01 00:00:00

  • Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin.

    abstract：:A competitive enzyme immunoassay for rat prolactin using an immunogen and a tracer obtained by recombinant DNA technology is described. Polyclonal antibodies were raised in rabbits immunized with a purified chimeric protein consisting of rat prolactin fused with two synthetic immunoglobulin G binding domains derived f...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(94)90264-x

    authors： Ezan E,Ducancel F,Gillet D,Drevet P,Ménez A,Grognet JM,Boulain JC

    更新日期：1994-03-10 00:00:00

  • M4HS2, a new human T-cell line for the efficient generation of human T-cell hybrids.

    abstract：:We have developed and characterised a variant of the human T-cell tumour, MOLT-4F, which is resistant to both 8-azaguanine and 6-thioguanine. This new cell line, M4HS2, is suitable for the efficient production of lymphokine-secreting human T-cell hybrids and is available for distribution. ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(86)90383-2

    authors： Gallagher G,Christie JF,Boyd E,Stimson WH

    更新日期：1986-06-10 00:00:00

  • ELISA methods for the analysis of antibody responses induced in multiple sclerosis patients treated with recombinant interferon-beta.

    abstract：:Upon treatment with protein therapeutics, a subset of patients will typically develop antibodies against the drug. These anti-drug antibodies can be of concern because they have the potential to alter the drug's therapeutic activity. In the case of relapsing-remitting multiple sclerosis (RRMS) patients receiving recom...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(99)00073-3

    authors： Brickelmaier M,Hochman PS,Baciu R,Chao B,Cuervo JH,Whitty A

    更新日期：1999-07-30 00:00:00

  • Monoclonal antibodies distinguishing alpha and beta forms of calcitonin gene-related peptide.

    abstract：:A panel of 18 monoclonal antibodies was raised to the human calcitonin gene related peptide (CGRP). Of these mabs, seven were specific for alpha CGRP and five for beta CGRP, while the remainder reacted with both alpha and beta CGRP. Nine different epitopes on CGRP were defined with these mabs. In addition, the mabs we...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(90)90115-c

    authors： Andrew DP,Bidgood TD,Bose C,Brown D,Galfre G,Sherwood M

    更新日期：1990-11-06 00:00:00

  • Ex vivo detection and enumeration of human antigen-specific CD8+ T lymphocytes using antigen delivery by a recombinant vaccinia expression vector and intracellular cytokine staining.

    abstract：:We describe an intracellular cytokine staining, flow cytometry based technique for quantitative and qualitative assessment of CD8+ T lymphocyte-mediated antigen specific immune responses. This adaptation of the technique first described by Suni et al. [J. Immunol. Methods, 212 (1998) 89; Cytometry, 40 (2000) 60] utili...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(02)00025-x

    authors： Speller SA,Warren AP

    更新日期：2002-04-01 00:00:00

  • Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.

    abstract：:Goat anti-mouse antibodies conjugated with colloidal 40 nm gold particles (G40) were used as secondary layers to stain human T lymphocytes in an attempt to extend the polarized light epi-illumination microscopic technique to flow cytometric multiparameter analysis. G40-labelled T cells were further stained with phycoe...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(87)90211-0

    authors： Festin R,Björklund B,Tötterman TH

    更新日期：1987-07-16 00:00:00

  • Multiplexed combinatorial tetramer staining in a mouse model of virus infection.

    abstract：:Use of fluorescently labelled multimers, particularly tetramers of peptide and MHC class I glycoprotein (pMHC-I) complexes, is essential for the analysis of CD8+ T cell immunity in basic research and clinical settings. A recently described combinatorial approach using pMHC-I multimers coupled to a unique combination o...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2010.06.003

    authors： Cukalac T,Valkenburg SA,La Gruta NL,Turner SJ,Doherty PC,Kedzierska K

    更新日期：2010-08-31 00:00:00

  • Production and characterisation of monoclonal antibodies to parathyroid hormone-related protein.

    abstract：:The production and characterisation of 17 monoclonal antibodies to human parathyroid hormone-related protein (PTH-rP) 1-34 is described. Five of the antibodies were shown to be of high avidity (Ka 4 X 10(10)-1.9 X 10(11) L/M) and able to detect 15-100 pg PTH-rP 1-34 per tube by RIA. None cross-reacted with PTH 1-34, a...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(90)90346-w

    authors： Ratcliffe WA,Hughes S,Gilligan MG,Heath DA,Ratcliffe JG

    更新日期：1990-02-20 00:00:00

  • Comparison of cell-based and non-cell-based assay platforms for the detection of clinically relevant anti-drug neutralizing antibodies for immunogenicity assessment of therapeutic proteins.

    abstract：:Anti-drug neutralizing antibodies (NAbs) formed due to unwanted immunogenicity of a therapeutic protein point towards a mature immune response. NAb detection is important in interpreting the therapeutic's efficacy and safety in vivo. In vitro cell-based NAb assays provide a physiological system for NAb detection, howe...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2015.02.006

    authors： Hu J,Wala I,Han H,Nagatani J,Barger T,Civoli F,Kaliyaperumal A,Zhuang Y,Gupta S

    更新日期：2015-04-01 00:00:00

  • Automatic detection of hypoxia in renal tissue stained with HIF-1alpha.

    abstract：OBJECTIVE:The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD:17 images of renal tissues from male rats of Winstar lineage; overall, there we...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2017.02.005

    authors： Buzin AR,Macedo ND,De Araujo IB,Nogueira BV,de Andrade TU,Endringer DC,Lenz D

    更新日期：2017-05-01 00:00:00

  • An improved method for the retrieval of cultured macrophages.

    abstract：:We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on dialysis membrane supports. Culture units are easily constructed from plastic petri dishes and dialysis membranes. Macrophages adhere readily but are detached easily from the dialysis membrane by mild mechanical agitatio...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(82)90322-2

    authors： Fallon MD,Kahn AJ,Ellerbrake D,Teitelbaum SL

    更新日期：1982-11-12 00:00:00

  • Use of GapmeRs for gene expression knockdowns in human primary resting CD4+ T cells.

    abstract：:Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not cause cell activat...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2019.112674

    authors： Abewe H,Deshmukh S,Mukim A,Beliakova-Bethell N

    更新日期：2020-01-01 00:00:00

  • Insights into the chicken IgY with emphasis on the generation and applications of chicken recombinant monoclonal antibodies.

    abstract：:The advantages of chicken (Gallus gallus domesticus) antibodies as immunodiagnostic and immunotherapeutic biomolecules has only been recently recognized. Even so, chicken antibodies remain less-well characterized than their mammalian counterparts. This review aims at providing a current overview of the structure, func...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.jim.2017.05.001

    authors： Lee W,Syed Atif A,Tan SC,Leow CH

    更新日期：2017-08-01 00:00:00

  • Free light chains: Eclectic multipurpose biomarker.

    abstract：:The production of antibodies is accompanied by a slight excess of synthesis of κ and λ immunoglobulin light chains; small amounts of them are released in the peripheral blood and can also be found in various body fluids, such as synovial fluid, cerebrospinal fluid, urine and saliva. They are rapidly filtered by the gl...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.jim.2017.09.005

    authors： Basile U,Gulli F,Gragnani L,Napodano C,Pocino K,Rapaccini GL,Mussap M,Zignego AL

    更新日期：2017-12-01 00:00:00

  • Simultaneous in situ detection of IgE receptors and cytoplasmic granules in murine cutaneous mast cells.

    abstract：:A method is described for the simultaneous in situ detection of surface receptors and cytoplasmic granules in mast cells of frozen sections of mouse skin. Surface IgE receptors are detected after saturation of the receptors with a murine monoclonal antibody of IgE isotype. The latter is subsequently detected by monosp...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(87)90121-9

    authors： Giorno R,Choi KL,Claman HN

    更新日期：1987-05-20 00:00:00