Abstract:
:We present a rapid and simple method for simultaneous quantitation and separation of mononuclear cell (MNC) subsets. When lymphoid cells are sensitized with monoclonal antibodies of the OK and Leu series, they rapidly form rosettes with ox erythrocytes (ORBC) coated with affinity-purified rabbit IgG against mouse IgG. Rosette-forming cells (RFC) may then be counted and separated from non-rosetting MNC by Isopaque-Ficoll gradient centrifugation. The yield and viability are close to 100% after ORBC lysis. Adherent cells do not interfere. Isolated T8+ and Leu3a+ cells were further tested: the purity was 97-99%, and the cells were functionally intact with respect to their modulating activity on the generation of immunoglobulin-secreting cells by MNC after stimulation with pokeweed mitogen.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Egeland T,Lea Tdoi
10.1016/0022-1759(82)90033-3subject
Has Abstractpub_date
1982-12-17 00:00:00pages
213-9issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(82)90033-3journal_volume
55pub_type
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