The use of non-human primates for production of antisera to human tumor-associated antigens.

abstract：:Two commercially available bioreactor systems, CELLine and miniPERM, were evaluated for their ability to support the production of monoclonal antibody (mAb) from a variety of murine hybridoma cell lines. Production and purity of mAbs were compared between the two systems and with mouse ascites tumour fluid generation....

journal_title：Journal of immunological methods

authors： Bruce MP,Boyd V,Duch C,White JR

更新日期：2002-06-01 00:00:00

abstract：:Drug-specific monoclonal antibodies (MAbs) were produced against the very small drug hapten (162.15 Da), 5-benzimidazolecarboxylic acid, an analogue of 2-(4-Thiazolyl)benzimidazole (TBZ) but lacking the thiol group. TBZ is widely used as a broad-spectrum anthelmintic in various animal species and humans and also as a ...

journal_title：Journal of immunological methods

authors： Moran E,O'Keeffe M,O'Connor R,Larkin AM,Murphy P,Clynes M

更新日期：2002-12-20 00:00:00

abstract：UNLABELLED:In this study the flow-cytometric crossmatch results were compared between fresh cells and cells processed by various cryopreservation and storage methods. Platelets from healthy donors were incubated with 12 sera containing platelet reactive antibodies as well as with 62 control sera from blood donors. Dire...

journal_title：Journal of immunological methods

authors： Döhlinger S,Humpe A,Connor J,Köhler M,Legler TJ

更新日期：2005-02-01 00:00:00

abstract：:We present the development and testing of a novel assay of lymphocyte adhesion based on time-resolved morphological measurements of intercellular aggregation. Homotypic lymphocyte aggregation is induced according to various protocols and monitored for several hours using video microscopy and time-lapse recording. Digi...

journal_title：Journal of immunological methods

authors： Munn LL,Glacken MW,McIntyre BW,Zygourakis K

更新日期：1993-11-05 00:00:00

abstract：:This study was designed to assess the use of flow cytometry to observe the binding, under physiological conditions, of anti-lipopolysaccharide (LPS) monoclonal antibodies (mAbs) to whole bacteria, and to compare this with the more conventional whole cell ELISA and immunoblotting techniques. The bacteria consisted of t...

journal_title：Journal of immunological methods

authors： Nelson D,Neill W,Poxton IR

更新日期：1990-10-19 00:00:00

abstract：:A method for quantitative RT-PCR using ELISA detection was developed and applied to the quantitation of IL-1 beta mRNA in clinical samples. To compensate for the 'tube effect' of RT-PCR, a synthetic RNA, pRSET RNA, was added to sample solutions as an internal standard and co-amplified with IL-1 beta mRNA. Sense primer...

journal_title：Journal of immunological methods

authors： Tsuruta H,Matsui S,Oka K,Namba T,Shinngu M,Nakamura M

更新日期：1995-03-27 00:00:00

abstract：:Ranibizumab (Lucentis®), a humanized antigen-binding fragment (Fab) monoclonal antibody that blocks VEGF-A activity, is currently approved for the treatment of several retinal degenerative diseases. The assessment of drug pharmacokinetics (PK) is essential for evaluating exposure as it relates to drug safety and effic...

journal_title：Journal of immunological methods

authors： Lowe J,Wakshull E,Shek T,Chuntharapai A,Elliott R,Rusit J,Maia M

更新日期：2018-10-01 00:00:00

abstract：:A leishmania amastigote radiobinding assay has been developed using organisms labeled with tritiated uracil. These labeled amastigotes resemble freshly isolated unlabeled amastigotes in metabolic activity, buoyant density, morphology, viability and their ability to transform into promastigotes. Organisms routinely inc...

journal_title：Journal of immunological methods

authors： Mosser DM

更新日期：1990-07-03 00:00:00

abstract：:The localisation of lipopolysaccharide-binding sites on erythrocytes with peroxidase-coupled LPS is described. LPS was isolated from Fusobacterium nucleatum (Fus MC-8) by phenol-water extraction. The LPS was coupled to horseradish peroxidase by the two-step method of Avrameas and Ternynck (1971). The biological and se...

journal_title：Journal of immunological methods

authors： Nygren H,Dahlen G

更新日期：1979-01-01 00:00:00

abstract：:Two highly sensitive immunoassays for measuring soluble DNA are described. These methods employ a purified mouse monoclonal anti-DNA antibody in enzyme-linked immunoassays. One assay is an antigen capture method utilizing immobilized antibody. Bound DNA is subsequently detected with biotinylated monoclonal anti-DNA an...

journal_title：Journal of immunological methods

authors： Du Clos TW,Rubin RL,Tan EM

更新日期：1986-04-17 00:00:00

abstract：:A competitive radioimmunoassay for immunoconglutinin (IK) was developed using isolated antostimulated rabbit IK labelled with 125I and glutaraldehyde-fixed sheep erythrocytes coated with fixed complement (EAC). IKs in test sera of human or animal origin produced specific inhibition of uptake of [125I]IK onto EAC and t...

journal_title：Journal of immunological methods

authors： Druguet M,Chayvialle JA,André C,Pepys MB

更新日期：1980-01-01 00:00:00

abstract：:A method is described for the purification of monoclonal antibody from mouse ascitic fluid. The fluid is clarified and the lipid removed using silicon dioxide powder, before the immunoglobulin is precipitated using polyethylene glycol. The method provides IgM antibody in high yield and good purity. In the case of IgG ...

journal_title：Journal of immunological methods

authors： Neoh SH,Gordon C,Potter A,Zola H

更新日期：1986-07-24 00:00:00

abstract：:The length distributions of the third complementarity determining region (CDR3) of the T cell receptor (TCR) beta chain were determined quantitatively from peripheral blood lymphocytes of healthy donors. RT-PCR products of 26 V beta families and subfamilies were analyzed by an A.L.F. DNA sequencer and Fragment Manager...

journal_title：Journal of immunological methods

authors： Liu D,Callahan JP,Dau PC

更新日期：1995-11-16 00:00:00

abstract：:A procedure to enhance incorporation of radioactive iodine into cell-surface proteins is described. The effects of chemical modification of cells with the iodinatible haptens N-succinimidyl-3-(4-hydroxyphenyl) propionate (Bolton-Hunter reagent) and methyl-p-hydroxybenzimidate HC1 (Wood reagent) are compared. The Wood ...

journal_title：Journal of immunological methods

authors： Wall KA,Fitch FW

更新日期：1985-02-28 00:00:00

abstract：:A method for the preservation of mouse peritoneal macrophages is described. Using 5% dimethylsulphoxide with either a cooling rate of 1 degree C/min or two-step cooling with 10 min interruption at -30 degrres C allows 80% survival as judged by pinocytic activity. An important finding was that the cells must be handled...

journal_title：Journal of immunological methods

authors： Foreman S,Schorlemmer HU,Farrant J,Pegg DE,Allison AC

更新日期：1977-01-01 00:00:00

abstract：:In the production of polyclonal antibody, a purification step is necessary which is often done by affinity chromatography. We present a biosensor system based on reflectometric interference spectroscopy (RIfS) to monitor the quantity and quality in terms of affinity and kinetic constants of the antibody during this pr...

journal_title：Journal of immunological methods

authors： Proll G,Kumpf M,Mehlmann M,Tschmelak J,Griffith H,Abuknesha R,Gauglitz G

更新日期：2004-09-01 00:00:00

abstract：:Natural killer (NK) cells are important mediators of virus- and tumor-specific immune responses. The transfection of genes into NK cells has been proven difficult and so far requires infection with virus-based vectors. Here, the application of a novel nonviral, electroporation-based gene transfer method is described f...

journal_title：Journal of immunological methods

authors： Trompeter HI,Weinhold S,Thiel C,Wernet P,Uhrberg M

更新日期：2003-03-01 00:00:00

abstract：:In this study we describe a fast, simple, and objective flow cytometry method to quantify simultaneously phagocytosis and Fc gamma R-mediated oxidative burst in human monocytes. Peripheral blood monocytes isolated by elutriation were sequentially incubated with 2',7'-dichlorofluorescein-diacetate (DCFH-DA) and sheep e...

journal_title：Journal of immunological methods

authors： Casado JA,Merino J,Cid J,Subira ML,Sanchez-Ibarrola A

更新日期：1993-02-26 00:00:00

abstract：:A panel of 18 monoclonal antibodies was raised to the human calcitonin gene related peptide (CGRP). Of these mabs, seven were specific for alpha CGRP and five for beta CGRP, while the remainder reacted with both alpha and beta CGRP. Nine different epitopes on CGRP were defined with these mabs. In addition, the mabs we...

journal_title：Journal of immunological methods

authors： Andrew DP,Bidgood TD,Bose C,Brown D,Galfre G,Sherwood M

更新日期：1990-11-06 00:00:00

abstract：:We developed a noncompetitive two-site sandwich ELISA to quantitate monoclonal antibodies in culture supernatant. This assay measures the initial enzyme activity rate during the first minute of the reaction, which ensures linear velocity relative to time and a progress curve slope proportional to analyte concentration...

journal_title：Journal of immunological methods

authors： Domínguez M,Moreno I,Toraño A

更新日期：2019-11-01 00:00:00

abstract：:The Farr technique has been compared with equilibrium dialysis in regard to its ability to measure antibody concentration and affinity. An excellent qualitative agreement between the two techniques was obtained. Quantitatively, the Farr technique tended to underestimate antibody concentration and overestimate antibody...

journal_title：Journal of immunological methods

authors： Kim YT,Kalver S,Siskind GW

更新日期：1975-02-01 00:00:00

abstract：:Western blotting is one of the most commonly and widely used methods for identifying the presence of an antigen in biochemical studies. The present study describes a novel 'coupled blotting' approach that allows antigen on dot and Western blots to be simultaneously probed with primary and secondary probing reagents. T...

journal_title：Journal of immunological methods

authors： Sundaram P

更新日期：1996-09-09 00:00:00

abstract：:Neutrophil accumulation at an inflammatory site or an infected tissue is dependent on the recognition of chemotactic peptides that bind to G-protein coupled receptors (GPCRs) exposed on the surface of the inflammatory cells. A GPCR activated by a chemoattractant quickly becomes refractory to further stimulation by lig...

journal_title：Journal of immunological methods

authors： Karlsson J,Bylund J,Movitz C,Björkman L,Forsman H,Dahlgren C

更新日期：2010-01-31 00:00:00

abstract：:Human peripheral blood or tonsil lymphocytes produce T cell growth factor (TCGF), when activated with neuraminidase (NA) and galactose oxidase (GO). Partial purification of NAGO-TCGF on Sepharose G-100 columns gave a TCGF-active fraction within the same molecular weight range as the conventional lectin-induced TCGF (a...

journal_title：Journal of immunological methods

authors： Berzins T,Wikén M,Hellström U,Perlmann P

更新日期：1983-10-28 00:00:00

abstract：:IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is ...

journal_title：Journal of immunological methods

authors： Janot-Sardet C,Assouline B,Cheynier R,Morre M,Beq S

更新日期：2010-02-28 00:00:00

abstract：:A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing....

journal_title：Journal of immunological methods

authors： Trudgett A

更新日期：1976-01-01 00:00:00

abstract：:The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific mouse monoclonal antibodies. Seven monoclonal antibodies (...

journal_title：Journal of immunological methods

authors： Kummer JA,Kamp AM,van Katwijk M,Brakenhoff JP,Radosević K,van Leeuwen AM,Borst J,Verweij CL,Hack CE

更新日期：1993-07-06 00:00:00

abstract：:The attachment of cells to plastic tissue culture plates is a widely used criterion of cell viability in microcytotoxicity assays. When cell suspensions of primary human colon carcinoma and melanoma cells which were of low initial viability (assessed by trypan blue exclusion) were allowed to adhere to tissue culture p...

journal_title：Journal of immunological methods

authors： Pullen GR,Chalmers PJ,Nind AP,Nairn RC

更新日期：1981-01-01 00:00:00

abstract：:Transduction of bone marrow stem cells with retroviral expression vectors represents a cheaper and more rapid alternative to conventional transgenesis for studies of in vivo gene function. However, achieving tissue-specific expression of genes inserted into retroviral vectors is notoriously difficult. We have develope...

journal_title：Journal of immunological methods

authors： Turner VM,Gardam S,Brink R

更新日期：2010-08-31 00:00:00

abstract：:A double-sandwich ELISA, developed for detection of IgM antibodies to the major surface protein of Toxoplasma gondii (P30), is proposed for the diagnosis of acute acquired toxoplasmosis. The method is based on the capture of serum IgM antibodies, which are revealed indirectly by the sequential addition of a Toxoplasma...

journal_title：Journal of immunological methods

authors： Cesbron JY,Capron A,Ovlaque G,Santoro F

更新日期：1985-10-24 00:00:00