Radioimmunoassay for immunoconglutinins.

Abstract:

:A competitive radioimmunoassay for immunoconglutinin (IK) was developed using isolated antostimulated rabbit IK labelled with 125I and glutaraldehyde-fixed sheep erythrocytes coated with fixed complement (EAC). IKs in test sera of human or animal origin produced specific inhibition of uptake of [125I]IK onto EAC and this was quantitated by reference to a calibration curve established with pure rabbit IK. Among 20 normal human sera the IK levels were: median 62 microgram/ml, range 41-170 microgram/ml and in 7 rat sera the levels were: median 71 microgram/ml, range 58-80 microgram/ml. Preliminary studies showed raised IK levels in the sera of patients with rheumatoid arthritis and with Crohn's disease.

journal_name

J Immunol Methods

authors

Druguet M,Chayvialle JA,André C,Pepys MB

doi

10.1016/0022-1759(80)90045-9

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

181-90

issue

3

eissn

0022-1759

issn

1872-7905

pii

0022-1759(80)90045-9

journal_volume

34

pub_type

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