Abstract:
:IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is important to develop a sensitive and reliable assay in order to investigate the potential role of this receptor in the regulation of IL-7 physiologic, physipathologic and therapeutic effects. We here report a fully validated method to measure sCD127 in human and simian plasma using a method based on ELISA MSD technology. We demonstrate that sCD127 is detectable at various levels in the plasma of healthy humans as well as in that of healthy Rhesus and Cynomolgus macaques (106.72, 205.26 and 366.95 ng/mL respectively). Moreover, as opposed to the sCD25/IL-2 tandem, we demonstrate that IL-7 treatment has no impact on sCD127 plasma concentration in patients infected by HIV.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Janot-Sardet C,Assouline B,Cheynier R,Morre M,Beq Sdoi
10.1016/j.jim.2009.12.003subject
Has Abstractpub_date
2010-02-28 00:00:00pages
115-23issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(09)00375-5journal_volume
353pub_type
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