Immunoadsorption of T cells onto glass beads using tetramolecular complexes of monoclonal antibodies.

Abstract:

:Tetramolecular monoclonal antibody complexes were used to selectively cross-link a subset of human peripheral blood T cells (CD8 positive) to glass beads coated with hapten (fluorescein) modified bovine serum albumin. Tetramolecular antibody complexes were prepared with anti-CD8 (Leu 2a), anti-FITC mouse IgG1 monoclonal antibodies and monoclonal rat anti-mouse IgG1. Optimum conditions for depletion of CD8 positive cells from peripheral blood mononuclear cell suspensions were determined with 1 ml columns. 90-99% of the CD8 positive cells could be removed with 0-17% non-specific adsorption of CD8 negative cells. The weakest link in this system was the bond between hapten-modified albumin and the glass beads. These results indicate that tetramolecular antibody complexes are useful for the specific immunoadsorption of cells to a defined affinity matrix.

journal_name

J Immunol Methods

authors

Thomas TE,Lansdorp PM

doi

10.1016/0022-1759(88)90361-4

subject

Has Abstract

pub_date

1988-09-13 00:00:00

pages

219-26

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(88)90361-4

journal_volume

112

pub_type

杂志文章
  • Quantitation of tumor growth in the ears of mice.

    abstract::Transplantation of ascitic P-815 mastocytoma cells intradermally in the ears of syngeneic DBA/2J mice allows a simple and precise quantitation of tumor growth by ear swelling. Tumor growth was retarded and then arrested in allogeneic DBA/1J mice, whereas syngeneic hosts died as a result of tumor dissemination in drain...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90086-2

    authors: Maia LC,Guerra RN,Rossi MI

    更新日期:1982-11-26 00:00:00

  • Development of a quantitative lateral-flow assay for rapid detection of fatty acid-binding protein.

    abstract::Using human heart-type fatty acid-binding protein (H-FABP) as an early cardiac marker to confirm or exclude a diagnosis of acute myocardial infarction (AMI) soon after admission requires a rapid assay system. Due to the requirement of skillful technicians and complicated assay procedures, most immunochemical assays fo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(03)00243-6

    authors: Chan CP,Sum KW,Cheung KY,Glatz JF,Sanderson JE,Hempel A,Lehmann M,Renneberg I,Renneberg R

    更新日期:2003-08-01 00:00:00

  • Immunosuppressive properties of anti-CD3 single-chain Fv and diabody.

    abstract::The mouse anti-human CD3 monoclonal antibody OKT3 is a potent immunosuppressive agent used in clinical transplantation. However, OKT3 therapy is associated with unpleasant and often serious side effects which appear to result from cytokine release, complement activation and a human anti-mouse antibody (HAMA) response....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2003.11.007

    authors: Le Gall F,Reusch U,Moldenhauer G,Little M,Kipriyanov SM

    更新日期:2004-02-01 00:00:00

  • Reduction of rigidity in human activated natural killer cells by thioglycollate treatment.

    abstract::Successful delivery of effector cells to tumor vasculature and the expression of cellular activities, such as extravasation and tumor infiltration are important aspects of the immune intervention of neoplastic disease. The structural rigidity of the effector cell, an indication of its cytoskeletal characteristics, pla...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(94)90332-8

    authors: Melder RJ,Jain RK

    更新日期:1994-09-30 00:00:00

  • Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins.

    abstract::The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific mouse monoclonal antibodies. Seven monoclonal antibodies (...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(93)90241-x

    authors: Kummer JA,Kamp AM,van Katwijk M,Brakenhoff JP,Radosević K,van Leeuwen AM,Borst J,Verweij CL,Hack CE

    更新日期:1993-07-06 00:00:00

  • Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform.

    abstract::Multiplex bead-based assays have many advantages over ELISA, particularly for the analyses of large quantities of samples and/or precious samples of limited volume. Although many commercial arrays covering multitudes of biologically significant analytes are available, occasionally the development of custom arrays is n...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2016.02.018

    authors: McDonald JU,Ekeruche-Makinde J,Ho MM,Tregoning JS,Ashiru O

    更新日期:2016-06-01 00:00:00

  • Malaria infections: what and how can mice teach us.

    abstract::Malaria imposes a horrific public health burden - hundreds of millions of infections and millions of deaths - on large parts of the world. While this unacceptable health burden and its economic and social impact have made it a focal point of the international development agenda, it became consensual that malaria contr...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jim.2014.05.001

    authors: Zuzarte-Luis V,Mota MM,Vigário AM

    更新日期:2014-08-01 00:00:00

  • Sheep erythrocyte rosettes in pigs, sheep, cattle and goats demonstrated in the presence of dextran.

    abstract::Formation of rosettes with sheep RBC by blood lymphocytes from young pigs was increased from 24.4% +/- 2.2 (mean +/- S.E.) in PBS to 54.1% +/- 1.9 in the presence of dextran. This increase was achieved without inducing appreciable rosette formation with other RBC which do not form rosettes in PBS. Lymphocytes which r...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(78)90146-1

    authors: Binns RM

    更新日期:1978-01-01 00:00:00

  • The effect of antigen cross-linking on the sensitivity of the enzyme-linked immunosorbent assay.

    abstract::To investigate the effects of antigen cross-linking on ELISA results, we assayed antibodies to several purified antigens, using microtitration trays coated with antigens in their native and cross-linked forms. Cross-linking was achieved by a carbodiimide promoted condensation reaction. For several antigens an increase...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(84)90389-2

    authors: Rotmans JP,Scheven BA

    更新日期:1984-05-11 00:00:00

  • Metal-enhanced PicoGreen fluorescence: application to fast and ultra-sensitive pg/ml DNA quantitation.

    abstract::In this paper we provide both a theoretical and experimental analysis of the sensitivity of a DNA quantitation assay using a fluorescent chromophore which non-covalently binds dsDNA. It is well-known that the range of DNA concentrations available for fluorescence quantitation depends on the concentration of the chromo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2010.09.011

    authors: Dragan AI,Bishop ES,Casas-Finet JR,Strouse RJ,Schenerman MA,Geddes CD

    更新日期:2010-10-31 00:00:00

  • Phagocyte interactions with Mycobacterium tuberculosis--Simultaneous analysis of phagocytosis, phagosome maturation and intracellular replication by imaging flow cytometry.

    abstract::Utilization of compounds that enhance the innate immune response against Mycobacterium tuberculosis is an attractive strategy for combating tuberculosis in the post-antibiotic era. Thus, it is crucial to develop methods that can be used to screen for such compounds and to investigate their mechanisms of action. Here, ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2015.10.003

    authors: Johansson J,Karlsson A,Bylund J,Welin A

    更新日期:2015-12-01 00:00:00

  • Monoclonal antibodies to cystic fibrosis antigen.

    abstract::A series of monoclonal antibodies has been prepared against a granulocyte antigen previously shown to be present in the serum of individuals homozygous or heterozygous for the cystic fibrosis (CF) gene. The specificity of the monoclonals was established by their ability to remove the antigen (CF antigen) from serum. T...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(86)90109-2

    authors: Hayward C,Chung S,Brock DJ,Van Heyningen V

    更新日期:1986-07-11 00:00:00

  • Isolation of porcine colostral immunoglobulins and preparation of monospecific anti-gamma, anti-alpha, and anti-mu chain antibodies using agarose-linked immunosorbents.

    abstract::A simple method for the simultaneous isolation of IgG2, IgG1, IgA and IgM from porcine colostral whey is described. It makes use of DEAE-cellulose chromatography followed by repeated Sepharose 6B exclusion chromatography after the removal of fat, casein and lipoproteins, Fused rocket immunoelectrophoresis is used to s...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(77)90058-8

    authors: Reyero C,Stockl W

    更新日期:1977-01-01 00:00:00

  • Detection and analysis of cytokine mRNA in tissues and cell lines.

    abstract::Many normal and pathological processes have been shown to be influenced by the quantitative and qualitative nature of cytokine production. Cytokines have also been shown to be central in modulating host responses to invasion by pathogens. Therefore detection and analysis of cytokine production are critical in the unde...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(97)00124-5

    authors: Walker KB

    更新日期:1998-03-01 00:00:00

  • An improved ELISA for anti-native DNA by elimination of interference by anti-histone antibodies.

    abstract::We evaluated an enzyme-linked immunosorbent assay (ELISA) for antibodies to native DNA (nDNA) in which protamine was used to link DNA to polystyrene. Elevated anti-nDNA was largely restricted to patients with systemic lupus erythematosus (SLE), and within this group good correlation between ELISA and the ammonium sulf...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(83)80009-x

    authors: Rubin RL,Joslin FG,Tan EM

    更新日期:1983-10-28 00:00:00

  • Delineation of IgM-receptor bearing human T and B lymphocytes using a direct plaque forming cell (PFC) assay.

    abstract::Based on the observation that binding of IgM cytophilic antibodies to lymphocytes is temperature dependent, a direct plaque forming cell (PFC) assay was developed to detect IgM-receptor bearing human peripheral blood T and B lymphocytes. Lymphocytes were passively sensitized with IgM anti-SRBC molecules at 4 degrees C...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(79)90330-2

    authors: Haegert DG

    更新日期:1979-01-01 00:00:00

  • Intrinsically stable IgG aggregates.

    abstract::Measurement of the absorbance due to light scattering at 40 nm proved to be a simple and reliable way of assessing the extent of aggregation in heat-treated IgG solutions. Using this technique the rate of aggregation was demonstrated to be markedly temperature dependent with a sharp inflexion in the curve close to 63 ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(81)90274-x

    authors: McCarthy D,Goddard DH,Pell BK,Holborow EJ

    更新日期:1981-01-01 00:00:00

  • Phage-based assay for rapid detection of bacterial pathogens in blood by Raman spectroscopy.

    abstract::Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2018.12.004

    authors: De Plano LM,Fazio E,Rizzo MG,Franco D,Carnazza S,Trusso S,Neri F,Guglielmino SPP

    更新日期:2019-02-01 00:00:00

  • Possible artifacts in the radioiodination of surface proteins of trypanosomatids.

    abstract::Three different methods for the radioiodination of surface proteins in trypanosomatids have been comparatively analyzed. Data interpretation in the lactoperoxidase-mediated method was impaired by autoiodination of the enzyme and its tight retention by the cells. In the enzymobeads method, iodinated enzymes were releas...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90051-5

    authors: Camargo EP,Barbieri CL,Jankevicius JV

    更新日期:1982-07-30 00:00:00

  • Quantitative analysis of Helicobacter pylori infection in a mouse model.

    abstract::Progress in elucidating the pathogenesis of Helicobacter pylori gastric infection and in developing an H. pylori vaccine will be aided by an animal model in which H. pylori can be reliably detected. To validate the use of the mouse model of H. pylori infection, we determined the susceptibility of three inbred strains ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(00)00215-5

    authors: Smythies LE,Chen JA,Lindsey JR,Ghiara P,Smith PD,Waites KB

    更新日期:2000-08-28 00:00:00

  • Detection of AMP-activated protein kinase in human sera by immuno-isoelectric focusing.

    abstract::AMPKalpha is a subunit of AMP-activated protein kinase (AMPK), a heterotrimeric enzyme that works as a fuel sensor activated in response to the depletion of cellular ATP. AMPKalpha is considered as a master switch in regulating glucose and lipid metabolism. Determining its presence in patient sera may help in diagnosi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2009.10.001

    authors: Malvoisin E,Livrozet JM,El Hajji-Ridah I,Makloufi D,Vincent N

    更新日期:2009-12-31 00:00:00

  • Resin-based micropipette tip for immunochromatographic assays in urine samples.

    abstract::A novel bioanalysis system based on immunochromatography was developed in connection with a nitrocellulose resin modified micropipette tip, such as ZipTip. The sandwich-type immunoassay was applied to our bioanalysis system. The simple handling of the micropipette enabled us to increase the sample volume and detect lo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2006.02.011

    authors: Yuhi T,Nagatani N,Endo T,Kerman K,Takata M,Konaka H,Namiki M,Takamura Y,Tamiya E

    更新日期:2006-05-30 00:00:00

  • A novel assay for detecting virus-specific antibodies triggering activation of Fcγ receptors.

    abstract::IgG responses are crucial in antiviral defence and instrumental for the serodiagnosis of infections. Fcγ receptors (FcγRs), which recognize the Fc-part of IgG, differ regarding their IgG binding affinity, IgG subclass preference, cellular expression profile and pathogen elimination mechanisms elicited upon activation....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.09.006

    authors: Corrales-Aguilar E,Trilling M,Reinhard H,Mercé-Maldonado E,Widera M,Schaal H,Zimmermann A,Mandelboim O,Hengel H

    更新日期:2013-01-31 00:00:00

  • Performance evaluation of the MBio Diagnostics point-of-care CD4 counter.

    abstract::The measurement of the absolute CD4 T-cell count is critical in the initial evaluation and staging of HIV-infected persons, yet access to this technology remains limited in many low resource settings where disease burden is highest. Here we evaluate the performance of a prototype point-of-care device (POC) to quantify...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.10.002

    authors: Logan C,Givens M,Ives JT,Delaney M,Lochhead MJ,Schooley RT,Benson CA

    更新日期:2013-01-31 00:00:00

  • Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye.

    abstract::Receptor-mediated antibody internalization is a key mechanism underlying several anti-cancer antibody therapeutics. Delivering highly toxic drugs to cancer cells, as in the case of antibody drug conjugates (ADCs), efficient removal of surface receptors from cancer cells and changing the pharmacokinetics profile of the...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2016.02.001

    authors: Nath N,Godat B,Zimprich C,Dwight SJ,Corona C,McDougall M,Urh M

    更新日期:2016-04-01 00:00:00

  • A rapid rosette technique for quantitation and separation of mononuclear cell subsets using monoclonal antibodies.

    abstract::We present a rapid and simple method for simultaneous quantitation and separation of mononuclear cell (MNC) subsets. When lymphoid cells are sensitized with monoclonal antibodies of the OK and Leu series, they rapidly form rosettes with ox erythrocytes (ORBC) coated with affinity-purified rabbit IgG against mouse IgG....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90033-3

    authors: Egeland T,Lea T

    更新日期:1982-12-17 00:00:00

  • A novel method for detecting antigen-specific human regulatory T cells.

    abstract::Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognitio...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.01.004

    authors: Ebert LM,MacRaild SE,Davis ID,Cebon J,Chen W

    更新日期:2012-03-30 00:00:00

  • Some characteristics of natural cytostatic mouse splenocytes.

    abstract::Murine B16 melanoma cells and metastasis variants of this tumor are resistant to NK activity mediated by normal splenocytes. The B16 cells are, however, sensitive to splenocyte-mediated cytostasis. Cytostasis was measured by a [125I]UDR incorporation-inhibition [(125I]UDR I-I) assay. The main characteristics of the [1...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(81)90066-1

    authors: Ehrlich R,Efrati M,Witz IP

    更新日期:1981-01-01 00:00:00

  • Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy.

    abstract::Near-infrared Raman spectroscopy may provide a highly sensitive, noninvasive means to identify activation status of leukocytes. The purpose of the current study was to establish Raman spectroscopic characteristics of T cell activation. Activation of the RsL.11 T cell clone in vitro with Con A resulted in specific decr...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2004.11.020

    authors: Mannie MD,McConnell TJ,Xie C,Li YQ

    更新日期:2005-02-01 00:00:00

  • Direct time-resolved fluorescence immunoassay for serum oestradiol based on the idiotypic anti-idiotypic approach.

    abstract::We report a novel competitive type immunoassay for oestradiol based on the idiotypic anti-idiotypic approach. This has been achieved by the production of an anti-idiotypic antibody (anti-Id) which is directed against the oestradiol binding site of the primary idiotypic antibody (Ab1). In this format the primary Ab1 wa...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(91)90068-q

    authors: Altamirano-Bustamante A,Barnard G,Kohen F

    更新日期:1991-04-08 00:00:00