DNA antibodies detected by microhemagglutination test in the diagnosis of systemic lupus erythematosus.

abstract：:Using in vitro established tumour cell lines attempts were made to assess the suitability of tetrazolium salt reduction (MTT) assay to replace the conventional radioactive base techniques for measuring cell proliferation and cell killing. The optimum conditions for MTT loading time, concentration of MTT and the time f...

journal_title：Journal of immunological methods

authors： Hussain RF,Nouri AM,Oliver RT

更新日期：1993-03-15 00:00:00

abstract：INTRODUCTION:Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune ...

journal_title：Journal of immunological methods

authors： Bargalló ME,Guardo AC,Maleno MJ,Miralles L,Egaña-Gorroño L,Escribà T,García F,Gatell JM,Arnedo M,Plana M

更新日期：2017-03-01 00:00:00

abstract：:We describe an intracellular cytokine staining, flow cytometry based technique for quantitative and qualitative assessment of CD8+ T lymphocyte-mediated antigen specific immune responses. This adaptation of the technique first described by Suni et al. [J. Immunol. Methods, 212 (1998) 89; Cytometry, 40 (2000) 60] utili...

journal_title：Journal of immunological methods

authors： Speller SA,Warren AP

更新日期：2002-04-01 00:00:00

abstract：:After centrifugation of heparinised whole blood on Hypaque-Ficoll medium (s.g. 1.114, Ferrante and Thong, 1980) the specific gravities were measured of the layers of medium above and below the band of polymorphonuclear leukocytes (PMN), as well as of the PMN and sedimented erythrocytes. The erythrocytes and PMN were a...

journal_title：Journal of immunological methods

authors： Bignold LP,Ferrante A

更新日期：1987-01-26 00:00:00

abstract：:Cyclophilins A and B (CyPA and CyPB) are known to be the main binding proteins for cyclosporin A (CsA), a potent immunosuppressive drug. Due to the high homology between the two proteins, antibodies to CyPB were found to cross-react with CyPA. In order to avoid this phenomenon, we raised specific antibodies against pe...

journal_title：Journal of immunological methods

authors： Allain F,Boutillon C,Mariller C,Spik G

更新日期：1995-01-13 00:00:00

abstract：:A rapid, direct method for the purification of sheep red cell rosetting lymphocytes (ERFC) was developed. The whole procedure, including rosette formation, density separation and hemolysis could be completed within 10 min. A mixture of human peripheral blood mononuclear cells (PBMC) and 2-aminoethylisothiouronium brom...

journal_title：Journal of immunological methods

authors： Mikamo S

更新日期：1988-03-16 00:00:00

abstract：:A double-sandwich ELISA, developed for detection of IgM antibodies to the major surface protein of Toxoplasma gondii (P30), is proposed for the diagnosis of acute acquired toxoplasmosis. The method is based on the capture of serum IgM antibodies, which are revealed indirectly by the sequential addition of a Toxoplasma...

journal_title：Journal of immunological methods

authors： Cesbron JY,Capron A,Ovlaque G,Santoro F

更新日期：1985-10-24 00:00:00

abstract：:Antigen-specific lymphocytes are important in the immune response to viral infection. Peripheral blood mononuclear cells (PBMC) are traditionally used as a source of effector cells in most immunological studies. We described here the use of the bispecific monoclonal antibodies (BSMAB) anti CD3:CD8 (CD3,8) and anti CD3...

journal_title：Journal of immunological methods

authors： Jones N,Agrawal D,Elrefaei M,Hanson A,Novitsky V,Wong JT,Cao H

更新日期：2003-03-01 00:00:00

abstract：:A mild procedure for the purification of rabbit Fab' antibodies is described. A small column of normal goat IgG-Sepharose 4B (3 cm x 5mm) was saturated with F(ab')2 prepared from rabbit anti-goat IgG, and anti-goat IgG Fab was eluted from the column by splitting the disulfide bond in the hinge of the F(ab')2 molecule ...

journal_title：Journal of immunological methods

authors： Ishikawa E,Yoshitake S

更新日期：1980-01-01 00:00:00

abstract：:Serologically and biochemically pure HLA antigens and other beta 2-microglobulin-associated proteins were isolated using a rapid immunoprecipitation technique with a monoclonal anti-beta 2-microglobulin reagent A88. Elution of the immunoprecipitate with beta 2-microglobulin isolated from kidney patients proved to be e...

journal_title：Journal of immunological methods

authors： LeClair K,Van Agthoven A,Terhorst C

更新日期：1981-01-01 00:00:00

abstract：:Receptor-mediated antibody internalization is a key mechanism underlying several anti-cancer antibody therapeutics. Delivering highly toxic drugs to cancer cells, as in the case of antibody drug conjugates (ADCs), efficient removal of surface receptors from cancer cells and changing the pharmacokinetics profile of the...

journal_title：Journal of immunological methods

authors： Nath N,Godat B,Zimprich C,Dwight SJ,Corona C,McDougall M,Urh M

更新日期：2016-04-01 00:00:00

abstract：:External proficiency testing programs designed to evaluate the performance of end-point laboratories involved in vaccine and therapeutic clinical trials form an important part of clinical trial quality assurance. Good clinical laboratory practice (GCLP) guidelines recommend both assay validation and proficiency testin...

journal_title：Journal of immunological methods

authors： Garcia A,Keinonen S,Sanchez AM,Ferrari G,Denny TN,Moody MA

更新日期：2014-07-01 00:00:00

abstract：:Giant liposomes (mean diameter 5.5 microns) composed of egg phosphatidylcholine or distearoyl phosphatidylcholine, phosphatidyl glycerol, cholesterol and triolein were prepared by a double emulsion technique. They were then mixed with model particulate (killed Bacillus subtilis, and killed Bacille Calmette-Guérin) and...

journal_title：Journal of immunological methods

authors： Antimisiaris SG,Jayasekera P,Gregoriadis G

更新日期：1993-12-03 00:00:00

abstract：:Monoclonal antibodies have been prepared against a synthetic peptide with a sequence corresponding to a repeated hydrophilic region of the protein core of the human MUC-2 gastrointestinal mucin. Peptide conjugates, prepared by glutaraldehyde cross-linking with keyhole limpet haemocyanin (KLH) and bovine serum albumin ...

journal_title：Journal of immunological methods

authors： Price MR,Sekowski M,Hooi DS,Durrant LG,Hudecz F,Tendler SJ

更新日期：1993-02-26 00:00:00

abstract：:Antibody molecules bind to antigen with six complementary determining region (CDR) loops, three of which are located on each variable heavy (V(H)) and light (V(L)) chains. Discovery and optimization of antibodies that bind antigen using in vitro techniques require diversification of one or more of these CDRs. Since an...

journal_title：Journal of immunological methods

authors： Leonard B,Sharma V,Smider V

更新日期：2006-12-20 00:00:00

abstract：:We have developed a double monoclonal sandwich enzyme immunoassay to measure smooth muscle myosin heavy chain (MHC). Analytical performance of the assay showed reliable detection of smooth muscle MHC in human sera. The mean of the smooth muscle MHC level in normal human sera was 0.9 +/- 0.9 ng/ml. In sera of patients ...

journal_title：Journal of immunological methods

authors： Katoh H,Suzuki T,Yokomori K,Suzuki S,Ohtaki E,Watanabe M,Yazaki Y,Nagai R

更新日期：1995-09-11 00:00:00

abstract：:Interferons-alpha and -beta (IFN-alpha and -beta) are cytokines that are widely known to induce potent anti-viral activity. However, it has become increasingly apparent that IFN-alpha and -beta exert a variety of other biological effects, including anti-tumour and immunomodulatory activities and are increasingly used ...

journal_title：Journal of immunological methods

authors： Mire-Sluis AR,Page LA,Meager A,Igaki J,Lee J,Lyons S,Thorpe R

更新日期：1996-09-09 00:00:00

abstract：:We have observed that the red reaction product of alkaline phosphatase immuno-conjugates and certain substrate preparations produces a brilliant red fluorescence that is visible by fluorescence microscopy using both fluorescein and rhodamine filter combinations. This provides a level of sensitivity greater than that o...

journal_title：Journal of immunological methods

authors： Murdoch A,Jenkinson EJ,Johnson GD,Owen JJ

更新日期：1990-08-28 00:00:00

abstract：:We have recently described a new colorimetric DNA enzyme immunoassay (DEIA) for detecting specific hybrids of complementary nucleic acids. This technology is based on an antibody that selectively recognizes double, but not single stranded DNA and therefore reveals the hybridization event independently from the DNA seq...

journal_title：Journal of immunological methods

authors： Bettinardi A,Imberti L,Sottini A,Primi D

更新日期：1992-01-21 00:00:00

abstract：:Radioimmunoassays are usually developed empirically since there have been few established rules which cover general RIA behaviour. Evidence is presented which enables the delineation of 2 types of RIA with distinct group characteristics. (1) Simple RIA, conforming to the law of mass-action kinetics, is a result of uni...

journal_title：Journal of immunological methods

authors： Willey KP

更新日期：1985-11-28 00:00:00

abstract：:To increase the selection efficiency and productivity of stable clones expressing recombinant antibody-IL-2 fusion protein, a dicistronic expression vector containing the anti-erbB2 scFv-Fc-IL-2 fusion gene followed by a weakened neo gene, was constructed to allow for the concurrent translation of the recombinant prot...

journal_title：Journal of immunological methods

authors： Chen L,Xie Z,Teng Y,Wang M,Shi M,Qian L,Hu M,Feng J,Yang X,Shen B,Guo N

更新日期：2004-12-01 00:00:00

abstract：:Rh0(D) antibodies which retain immune specificity after radiolabeling were prepared by a procedure which does not require IgG isolation from serum, requires 10-fold less isotope than conventional techniques and yields antibody solutions of defined composition. The method involves radioiodination of IgG on immobilized ...

journal_title：Journal of immunological methods

authors： Victoria EJ,Wasserman SL,Kleeman JE,Masouredis SP

更新日期：1982-01-01 00:00:00

abstract：:Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous methods to develop a flow...

journal_title：Journal of immunological methods

authors： Charles ED,Orloff MI,Dustin LB

更新日期：2011-01-05 00:00:00

abstract：:Simplified isolation procedures are described for the parenchymal cell of the liver and the major non-parenchymal cell, the Kupffer cell. Hepatocytes are obtained in a purity of approximately 100%; a yield 10 X 10(6) cells/g liver tissue and the viability is greater than 85%. The recovery of Kupffer cells is 82%, viab...

journal_title：Journal of immunological methods

authors： Page DT,Garvey JS

更新日期：1979-01-01 00:00:00

abstract：:A leishmania amastigote radiobinding assay has been developed using organisms labeled with tritiated uracil. These labeled amastigotes resemble freshly isolated unlabeled amastigotes in metabolic activity, buoyant density, morphology, viability and their ability to transform into promastigotes. Organisms routinely inc...

journal_title：Journal of immunological methods

authors： Mosser DM

更新日期：1990-07-03 00:00:00

abstract：:A new, relatively simple, spectrophotometric technique has been developed which is useful for accurately determining the extent of chromophore labeling of proteins. Often the absorbance spectra and extinction coefficients of dye/protein conjugates are strongly affected by changes in the chromophore microenvironment th...

journal_title：Journal of immunological methods

authors： Meadows DL,Shafer JS,Schultz JS

更新日期：1991-10-25 00:00:00

abstract：:In addition to their known implication in allergy studies, IgE antibodies are becoming an increasingly interesting antibody class in cancer research. However, large-scale purification of IgE antibodies still poses substantial challenges, as they cannot be purified using techniques commonly used for other immunoglobuli...

journal_title：Journal of immunological methods

authors： Vukovic N,Harraou S,van Duijnhoven SMJ,Zaiss DM,van Elsas A

更新日期：2021-02-01 00:00:00

abstract：:A mixed hemadsorption (MH) test for naturally acquired rubella immunity and immune responses after rubella vaccination has been compared with the hemagglutination-inhibition (HI) test, the immunodiffusion (ID) test, the neutralization test (NT), the hemolysis-in-gel (HIG) test and the indirect immunofluorescence (IF) ...

journal_title：Journal of immunological methods

authors： Grandien M,Espmark A,Grillner L

更新日期：1980-01-01 00:00:00

abstract：:The advantages of chicken (Gallus gallus domesticus) antibodies as immunodiagnostic and immunotherapeutic biomolecules has only been recently recognized. Even so, chicken antibodies remain less-well characterized than their mammalian counterparts. This review aims at providing a current overview of the structure, func...

journal_title：Journal of immunological methods

authors： Lee W,Syed Atif A,Tan SC,Leow CH

更新日期：2017-08-01 00:00:00

abstract：:It is known that membranoproliferative glomerulonephritis (MPGN), hypocomplementaemia and C3 nephritic factor (C3NeF) are closely related to each other, and the presence or absence of C3NeF in the serum is important for evaluating the nature of MPGN. However, some difficulties have been encountered in detecting this f...

journal_title：Journal of immunological methods

authors： Ohi H,Watanabe S,Fujita T,Seki M,Hatano M

更新日期：1990-07-20 00:00:00