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In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter.

Abstract：

:This paper describes a new and easy to handle reusable minifermenter for high-density culture of hybridoma and other cells. The culture apparatus is composed of two modules: a 40 ml disposable cell culture and antibody production chamber (the 'production module') and a 550 ml medium reservoir (the 'supply module'). The two modules are separated from each other by a dialysis membrane allowing passage of low molecular mass nutrients and metabolites. The monoclonal antibodies are produced and enriched in the production module. The outer part of this module is made from a thin gas-permeable silicone rubber membrane allowing exchange of gases (oxygen and carbon dioxide). To start the culture, the cells are injected into the production module through ports in the silicone rubber which are equipped with Luer Lock connectors. Samples can be removed in the same way. For culturing, the minifermenter is rolled on a roller apparatus in a carbon dioxide-supplied incubator. Depending on the individual properties of the hybridoma cells cultured, cell densities of more than 10 x 10(6) (in some cases up to 35 x 10(6)) cells per ml and monoclonal antibody concentrations of several mg per ml can be obtained in the new minifermenter. On average, 61 mg (range: 9-159 mg) could be produced within 1-4 weeks. In terms of their properties the monoclonal antibodies produced in the new modular minifermenter were indistinguishable from antibodies prepared from ascitic fluid or from the supernatant of conventional stationary culture. The culture method is a useful alternative to the in vivo production method in mice. In addition, it represents a completely new, inexpensive and easy to handle general solution to the problem of culturing cells in high density and obtaining cellular products in high concentrations.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Falkenberg FW,Weichert H,Krane M,Bartels I,Palme M,Nagels HO,Fiebig H

doi

10.1016/0022-1759(94)00266-y

subject

Has Abstract

pub_date

1995-02-13 00:00:00

pages

13-29

issue

eissn

0022-1759

issn

1872-7905

pii

002217599400266Y

journal_volume

179

pub_type

杂志文章

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In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter.