Abstract:
:The cholesterol-rich lipid rafts on T cell membrane play important role in the formation of T cell receptor (TCR) signalosome upon receptor ligation. Analytical studies on the kinetics of lipid rafts formation and recruitment of protein receptors to lipid rafts are still limited by the use of a large number of cells. Herein, we describe a strategy for detecting fine alterations in the amount and distribution of glycosphingolipid (GM1) lipid rafts, and in the formation of GM1-TCR complexes in detergent-insoluble and -soluble compartments of the T cell membrane from a relative low number of cells. Using this strategy, we found that the GM1 moiety was physically associated with TCR in both detergent-insoluble and -soluble fractions. Shortly after ligation of CD3/TCR complex with a soluble CD3- epsilon mAb, the TCR was found mainly in the detergent-soluble fraction of the T cell membrane.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Thomas S,Kumar RS,Casares S,Brumeanu TDdoi
10.1016/s0022-1759(03)00014-0keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
161-8issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175903000140journal_volume
275pub_type
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