Abstract:
:The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific mouse monoclonal antibodies. Seven monoclonal antibodies (mAb) were raised against granzyme A, which all recognized the same or overlapping epitopes. They reacted specifically in an immunoblot of interleukin-2 (IL-2) stimulated PBMNC with a disulfide-linked homodimer of 43 kDa consisting of 28 kDa subunits. Seven mAb against granzyme B were obtained, which could be divided into two groups, each recognizing a different epitope. On an immunoblot, all mAb reacted with a monomer of 33 kDa protein. By immunohistochemistry, these mAb could be used to detect granzymes A and B expression in activated CTL and NK cells. The availability of these mAb may facilitate studies on the role of human cytotoxic cells in various immune reactions and may contribute to a better understanding of the role of granzymes A and B in the cytotoxic response in vivo.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Kummer JA,Kamp AM,van Katwijk M,Brakenhoff JP,Radosević K,van Leeuwen AM,Borst J,Verweij CL,Hack CEdoi
10.1016/0022-1759(93)90241-xsubject
Has Abstractpub_date
1993-07-06 00:00:00pages
77-83issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(93)90241-Xjournal_volume
163pub_type
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journal_title:Journal of immunological methods
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