Abstract:
:A technique for detecting the nitroblue tetrazolium-reducing activity of human polymorphonuclear leukocyte enzymes separated on polyacrylamide gels is reported. Slices of gel are incubated in buffer containing nitroblue tetrazolium and either NADPH or NADH. Enzymes capable of reducing nitroblue tetrazolium form a dark band due to the formation of the insoluble formazan. By this technique the soluble fraction of human polymorphonuclear leukocyte sonicates was shown to contain several enzymes capable of reducing nitroblue tetrazolium in the presence of NADPH and NADH.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Phillips WA,Shelton MJ,Hosking CSdoi
10.1016/0022-1759(82)90058-8subject
Has Abstractpub_date
1982-10-29 00:00:00pages
175-81issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(82)90058-8journal_volume
54pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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