Hepatocyte growth factor reverses the TGF-beta-induced growth inhibition of CCL-64 cells. A novel bioassay for HGF and implications for the TGF-beta bioassay.


:The influence of human hepatocyte growth factor (HGF) on the transforming growth factor beta (TGF-beta) bioassay CCL-64 was examined. HGF induced proliferation of the CCL-64 cells and potently counteracted TGF-beta-induced growth inhibition. HGF was not inactivated by transient acidification to pH 2, a commonly used procedure to activate latent TGF-beta. HGF was a stronger mitogen for the mink lung cells than epidermal growth factor (EGF), a known stimulator of CCL-64 cell growth. Costimulation of the cells by these two cytokines resulted in an additive effect on proliferation. In complex biological fluids containing large amounts of HGF, the TGF-beta concentration can be underestimated when determined by the CCL-64 assay. When a fixed amount of TGF-beta is added, the CCL-64 cells can be used as a reliable bioassay for HGF with a sensitivity of about 1 ng/ml.


J Immunol Methods


Börset M,Waage A,Sundan A




Has Abstract


1996-01-16 00:00:00














  • A shorter and more specific oral sensitization-based experimental model of food allergy in mice.

    abstract::Cow's milk protein allergy (CMPA) is one of the most prevalent human food-borne allergies, particularly in children. Experimental animal models have become critical tools with which to perform research on new therapeutic approaches and on the molecular mechanisms involved. However, oral food allergen sensitization in ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bailón E,Cueto-Sola M,Utrilla P,Rodríguez-Ruiz J,Garrido-Mesa N,Zarzuelo A,Xaus J,Gálvez J,Comalada M

    更新日期:2012-07-31 00:00:00

  • Lymphocyte subset enumeration in HIV seronegative and HIV-1 seropositive adults in Dar es Salaam, Tanzania: determination of reference values in males and females and comparison of two flow cytometric methods.

    abstract::The level of CD4(+) T-lymphocytes represents a useful marker with which to monitor the progression of HIV infection. Sex and geographical differences in the reference values of lymphocyte subsets have been reported. We have compared two flow cytometric methods (MultiSET and SimulSET) for the quantification of lymphocy...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Urassa WK,Mbena EM,Swai AB,Gaines H,Mhalu FS,Biberfeld G

    更新日期:2003-06-01 00:00:00

  • Evaluation of single- and dual antigen delayed fluorescence immunoassay in comparison to an ELISA and the in vivo toxin neutralisation test for detection of diphtheria toxin antibodies.

    abstract::An evaluation of the delayed fluorescence immunoassay (Delfia) against an ELISA method for determination of diphtheria antitoxin levels in serum was performed. The Delfia was also validated in the in vivo toxin neutralisation test (Txn) in rabbits. Two variants of the Delfia were studied, a single-antigen Delfia (sDel...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bonin E,Tiru M,Hallander H,Bredberg-Rådén U

    更新日期:1999-11-19 00:00:00

  • The use of 7-amino-actinomycin D in the analysis of Candida albicans phagocytosis and opsonization.

    abstract::We describe the use of 7-amino-actinomycin D (7AAD) to measure phagocytosis and the opsonizing capacity of serum. Heat-inactivated Candida albicans was previously stained with 7AAD and incubated with resident peritoneal macrophages. The samples were analyzed by flow cytometry and phagocytic cells were identified by th...

    journal_title:Journal of immunological methods



    authors: Ortega E,Algarra I,Serrano MJ,Alvarez de Cienfuegos G,Gaforio JJ

    更新日期:2001-07-01 00:00:00

  • Development of a radioimmunoassay for human milk-fat-globule membrane glycoprotein. Its quantitation in spent media from both primary and established mammary and non-mammary epithelial cell lines.

    abstract::A radioimmunoassay (RIA) was developed for the quantitative measurement of a glycoprotein which was purified from human milk-fat-globule membrane (MFGM) and termed MFGM-pg 70. The assay with a sensitivity of detecting 30 ng of MFGM-pg 70/ml was employed to quantitate the levels of MFGM-gp 70 shed in supernatants from ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Imam A,Neville AM

    更新日期:1986-11-20 00:00:00

  • Application of differential display to immunological research.

    abstract::The majority of immunological processes are mediated by cell-to-cell contact or receptor-ligand interactions that transmit intracellular signals and affect the regulation of transcription in the nucleus. As a consequence, precursor cells develop into their respective lineages and cells differentiate further during an ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审


    authors: Ali M,Markham AF,Isaacs JD

    更新日期:2001-04-01 00:00:00

  • Affinity purification of IgG monoclonal antibodies using the D-PAM synthetic ligand: chromatographic comparison with protein A and thermodynamic investigation of the D-PAM/IgG interaction.

    abstract::This study investigates the applicability of D-PAM, the inverso form of the Protein A Mimetic synthetic peptide affinity ligand (PAM) obtained from the screening of a multimeric combinatorial peptide library, in monoclonal IgG isolation from ascitic fluids and cellular supernatants. D-PAM affinity columns, prepared by...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: D'Agostino B,Bellofiore P,De Martino T,Punzo C,Rivieccio V,Verdoliva A

    更新日期:2008-04-20 00:00:00

  • A fluorescence quenching technique using trypan blue to differentiate between attached and ingested glutaraldehyde-fixed red blood cells in phagocytosing murine macrophages.

    abstract::A fluorescence quenching method, using trypan blue, is described for quantifying the ingestion of either glutaraldehyde-fixed sheep red blood cells or antibody coated glutaraldehyde-fixed sheep red blood cells by murine peritoneal macrophages. This method is based on the observations that glutaraldehyde-fixed red bloo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Loike JD,Silverstein SC

    更新日期:1983-02-25 00:00:00

  • The simultaneous direct detection of Mycoplasma pneumoniae and Legionella pneumophila antigens in sputum specimens by a monoclonal antibody immunoblot assay.

    abstract::An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Madsen RD,Saeed FA,Coates SR

    更新日期:1987-11-05 00:00:00

  • The development of species-specific immunodiagnostics for Stachybotrys chartarum: the role of cross-reactivity.

    abstract::Mold contamination and exposure to fungi in indoor environments has been associated with various adverse health effects but little is known about the significance of individual fungal species in the initiation or exacerbation of such effects. Using Stachybotrys chartarum as a model fungus we sought to demonstrate that...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Schmechel D,Simpson JP,Beezhold D,Lewis DM

    更新日期:2006-02-20 00:00:00

  • Rapid dissociation of adherent human tumor cells by ultrasound.

    abstract::Cultured human melanoma and gastrointestinal carcinoma cells were detached from substrate and further dissociated by placing the culture vessel into a water-filled ultrasonic cleaner (43 kHz) and sonicating it for 10-50 s. Plating efficiency and long-term growth of three melanoma cell lines were similar after ultrasou...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Menssen HD,Herlyn M,Rodeck U,Koprowski H

    更新日期:1987-11-23 00:00:00

  • A comparison between ELISPOT methods for the detection of cytokine producing cells: greater sensitivity and specificity using ELISA plates as compared to nitrocellulose membranes.

    abstract::We have used the ELISPOT method employing plastic ELISA plates without substrate in agar for the detection of single cells producing interferon-gamma (IFN-gamma) and interleukin-4 (IL-4). When using PBMC directly stimulated in the assay wells with T cell mitogens it was possible to measure production of human IFN-gamm...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Rönnelid J,Klareskog L

    更新日期:1997-01-15 00:00:00

  • Primed lymphocyte testing in pigs: conditions for the generation of primed cells and their response characteristics.

    abstract::Pig peripheral blood lymphocytes, typed for LD antigens by negative reactions to homozygous cells in primary MLRs and for SD antigens by complement-mediated lymphocytotoxicity, were sensitised in vitro against X-irradiated lymphocytes. These "primed" cells were rechallenged with lymphocytes possessing a range of LD an...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Pawelec GP

    更新日期:1978-01-01 00:00:00

  • High level expression of a human rabies virus-neutralizing monoclonal antibody by a rhabdovirus-based vector.

    abstract::Humans exposed to rabies virus must be promptly treated by passive immunization with anti-rabies antibody and active immunization with rabies vaccine. Currently, antibody prepared from pooled human serum or from immunized horses is utilized. However, neither of these reagents are readily available, entirely safe, or c...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Morimoto K,Schnell MJ,Pulmanausahakul R,McGettigan JP,Foley HD,Faber M,Hooper DC,Dietzschold B

    更新日期:2001-06-01 00:00:00

  • A simple multiple chamber apparatus for measuring chemotaxis of polymorphonuclear leukocytes utilizing centrifugation of the chambers before incubation.

    abstract::A simple apparatus for measuring chemotaxis is described which contains thirty chambers per unit. Only very few cells and small volumes of chemotactic factor solution are required per chamber. For satisfactory results the apparatus must be centrifuged briefly prior to incubation. Under these conditions results can be ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Swanson MJ

    更新日期:1977-01-01 00:00:00

  • Determination of phenobarbital by radioimmunoassay.

    abstract::A radioimmunoassay for phenobarbital has been studied. Antiphenobarbital antisera were obtained by repeated immunization of rabbits with p-succinamidophenobarbital conjugated to bovine serum albumin. Less than 0.2 pmol of phenobarbital could be measured by this procedure. The specificity of the antibodies was directed...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Yamaoka A,Takatori T

    更新日期:1979-01-01 00:00:00

  • A novel antibody overlay technique for two-dimensional immunoelectrophoresis.

    abstract::A new antibody overlay technique for 2-dimensional immunoelectrophoresis (2-D-IEP) is described. After the first dimension electrophoresis of the antigen, the desired amount of antiserum is applied to the initial agarose layer and then evenly distributed over a defined surface of the gel with a PVC film. This modifica...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Lebecq JC,Salhi SL,Bastide JM

    更新日期:1984-02-10 00:00:00

  • DNA antibodies detected by microhemagglutination test in the diagnosis of systemic lupus erythematosus.

    abstract::A method of tanned erythrocyte agglutination of wide applicability is described for the study of DNA antibodies. The practical aspects of the method have been explored in estimation of antibodies to DNA in human cases of systemic lupus erythematosus, discoid lupus erythematosus, rheumatoid arthritis, other collagenose...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Depiante-Depaoli M,Riera CM,Yantorno C,Risemberg A,Strussberg A

    更新日期:1978-01-01 00:00:00

  • An objective filter-based, enzymatic method for the in vivo measurement of the migration of human polymorphonuclear leucocytes.

    abstract::Incorporation of control valves into a previously described device enabled us to regulate the formation of 8 suction blisters on the upper surface of the forearm in adult human volunteers. After the removal of the raised epidermis and blister fluid, uniform areas of denuded dermis were obtained by placing hollow adhes...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Fernandes AC,Anderson R,Ras GJ

    更新日期:1985-11-07 00:00:00

  • A novel method to efficiently isolate medullary thymic epithelial cells from murine thymi based on UEA-1 MicroBeads.

    abstract:OBJECTIVE:The central mechanism for establishing a self-tolerant and functional T cell repertoire includes the promiscuous expression of otherwise tissue-restricted proteins by medullary thymic epithelial cells (TEC). We here demonstrate a novel and highly efficient method for isolating this rare key cell type. METHOD...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Dohr D,Engelmann R,Müller-Hilke B

    更新日期:2019-04-01 00:00:00

  • The use of denaturing conditions for gel diffusion of insoluble epidermal proteins.

    abstract::Modifications of the classical technique for double diffusion in agar plates are presented. These procedures allow insoluble proteins to be dissolved in a variety of denaturing solvents and antibody production specificity to be monitored by the appearance of precipitin lines in agarose gels as in the conventional Ouch...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Lee LD,Baden HP

    更新日期:1977-01-01 00:00:00

  • Rosétte formation between human b lymphocytes and zymosan-C3 complexes using different complement sources.

    abstract::Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not di...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Kajdacsy-Balla AA,Mendes NF

    更新日期:1976-01-01 00:00:00

  • Contaminant bands on SDS-polyacrylamide gel electrophoresis are recognised by antibodies in normal human serum and saliva.

    abstract::Contamination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis samples has commonly produced artefactual bands when sensitive detection systems, such as silver staining or immunoblotting procedures, have been used. Evidence suggests that these bands originate from human skin contamination of reagents and ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Riches PG,Polce B,Hong R

    更新日期:1988-05-25 00:00:00

  • Autoantibodies directed against labile epitopes on cell surface proteins in autoimmune disease patients: proposal of a novel ELISA for the detection of anti-endothelial cell antibodies.

    abstract::This article describes a novel method for detecting anti-endothelial cell antibodies (AECAs). Sera from patients with systemic vasculitis or inflammatory conditions have been reported to contain antibodies (Abs) that bind to endothelial cells (EC), i.e., AECAs. AECAs are known to play immunogenic effects by triggering...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Miura K,Aoun K,Yoshida S,Kurosawa Y

    更新日期:2012-08-31 00:00:00

  • Immunoperoxidase procedures to detect monoclonal antibodies against cell surface antigens. Quantitation of binding and staining of individual cells.

    abstract::An immunoperoxidase method has been developed which allows accurate and sensitive quantitation of the binding of monoclonal antibodies to cell surface antigens. Monolayers of fixed cells were prepared in wells of Terasaki micro-test plates and monoclonal antibodies bound to cell surface antigens were identified by the...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Lansdorp PM,Astaldi GC,Oosterhof F,Janssen MC,Zeijlemaker WP

    更新日期:1980-01-01 00:00:00

  • Simultaneous screening for two different antibodies in ELISA by combining two solid phases: microtiter plate and Falcon assay screening test system lid.

    abstract::An enzyme-linked immunosorbent assay (ELISA) is described that used a combination of 2 solid phases: a microtiter plate covered with a Falcon assay screening test (F.A.S.T.) system lid. By coating each solid phase with a different antigen, it was possible to simultaneously detect 2 different antibodies. The results of...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Ianconescu M

    更新日期:1985-02-11 00:00:00

  • Isolation of specific antibody under conditions of low ionic strength.

    abstract::Specific rabbit and goat antibodies to tobacco mosaic virus, bovine serum albumin and human gamma globulin have been isolated by acid dissociation from the antigen in the absence of salt. Maximum recovery of 96-100% of active antibody occurred at pH 2.8-2.9. The amount of antibody recovered within any given pH range w...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Hardie G,van Regenmortel MH

    更新日期:1977-01-01 00:00:00

  • Comparison between multiplex assays for autoantibody detection in systemic lupus erythematosus.

    abstract::The performance of immunoassays for the detection of autoantibodies is of critical importance to the diagnosis and assessment of patients with systemic lupus erythematosus (SLE). Our objective was to compare 3 multiplexed assays for measurement of multiple autoantibodies and their association with global disease activ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Hanly JG,Su L,Farewell V,Fritzler MJ

    更新日期:2010-06-30 00:00:00

  • An indirect antibody assay using haptenated antigen and 125i-labelled anti-hapten antibody.

    abstract::Hapten (trinitrophenyl) was coupled to antigen (ovalbumin). The haptenated antigen was bound by anti-ovalbumin antibody and binding was quantitated with 125I-labelled anti-hapten antibodies. Thus, with a single radioactive reagent antibodies against a variety of antigens can be detected, whilst the problems inherent i...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Aalberse RC

    更新日期:1978-01-01 00:00:00

  • Direct determination of molecular ratios of peptides coupled via N-succinimidyl 3-(2-pyridyldithio)propionate to carrier proteins using high performance liquid chromatography.

    abstract::A simple, rapid and reproducible method is presented for direct determination of the substitution ratio of a carrier protein with a synthetic nonradioactively labeled peptide. The peptide was covalently linked by a thiol group of a cysteine residue to the immunogenic carrier protein using the heterobifunctional reagen...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bläsi U,Linke RP,Lubitz W

    更新日期:1988-04-06 00:00:00