Hepatocyte growth factor reverses the TGF-beta-induced growth inhibition of CCL-64 cells. A novel bioassay for HGF and implications for the TGF-beta bioassay.


:The influence of human hepatocyte growth factor (HGF) on the transforming growth factor beta (TGF-beta) bioassay CCL-64 was examined. HGF induced proliferation of the CCL-64 cells and potently counteracted TGF-beta-induced growth inhibition. HGF was not inactivated by transient acidification to pH 2, a commonly used procedure to activate latent TGF-beta. HGF was a stronger mitogen for the mink lung cells than epidermal growth factor (EGF), a known stimulator of CCL-64 cell growth. Costimulation of the cells by these two cytokines resulted in an additive effect on proliferation. In complex biological fluids containing large amounts of HGF, the TGF-beta concentration can be underestimated when determined by the CCL-64 assay. When a fixed amount of TGF-beta is added, the CCL-64 cells can be used as a reliable bioassay for HGF with a sensitivity of about 1 ng/ml.


J Immunol Methods


Börset M,Waage A,Sundan A




Has Abstract


1996-01-16 00:00:00














  • Epitope mapping and affinity purification of monospecific antibodies by Escherichia coli cell surface display of gene-derived random peptide libraries.

    abstract::We report a method for the precise mapping of linear epitopes by presenting a peptide library on the surface of Escherichia coli cells. A random library of gene fragments derived from the classical swine fever virus (CSFV) envelope protein E(rns) was generated by DNAse I cleavage and cloned into a specially designed b...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Christmann A,Wentzel A,Meyer C,Meyers G,Kolmar H

    更新日期:2001-11-01 00:00:00

  • Ethidium bromide in the detection of antibodies to DNA and of circulating DNA by two-stage counterimmunoelectrophoresis.

    abstract::In an attempt to overcome the limitations of counterimmunoelectrophoresis in the detection of precipitating anti-DNA antibodies or circulating DNA, ethidium bromide has been used to increase the visibility of the precipitin lines and to confirm their specificity. ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Riboldi P,Asero R

    更新日期:1985-12-17 00:00:00

  • Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins.

    abstract::The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific mouse monoclonal antibodies. Seven monoclonal antibodies (...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Kummer JA,Kamp AM,van Katwijk M,Brakenhoff JP,Radosević K,van Leeuwen AM,Borst J,Verweij CL,Hack CE

    更新日期:1993-07-06 00:00:00

  • Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.

    abstract::Many labs wish to measure cytokines in an accurate, reproducible, and rapid manner. An antibody-based membrane array for measuring cytokines has been developed based on the same technology as the traditional ELISA. The aim of this study was to compare results obtained with the traditional ELISA method with those from ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Copeland S,Siddiqui J,Remick D

    更新日期:2004-01-01 00:00:00

  • Cell-specific heterogeneity in sensitivity of phosphatidylinositol-anchored membrane antigens to release by phospholipase C.

    abstract::Cell surface antigens thought to be linked to the membrane via phosphatidylinositol (PI) are incompletely, and variably, released by treatment with phosphatidylinositol-specific phospholipase C (PI-PLC). The basis for this was investigated with cloned tumor cell lines and PI-PLCs isolated from two species of bacteria....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Low MG,Stiernberg J,Waneck GL,Flavell RA,Kincade PW

    更新日期:1988-10-04 00:00:00

  • PCR amplification of the functional immunoglobulin heavy chain variable gene from a hybridoma in the presence of two aberrant transcripts.

    abstract::Single chain antibody fragment genes are commonly created by splicing together the immunoglobulin light chain (VL) and heavy chain variable (VH) genes of a monoclonal antibody produced by a hybridoma. Selective PCR amplification of the functional immunoglobulin variable gene rearrangements can be complicated by the ex...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Irani Y,Tea M,Tilton RG,Coster DJ,Williams KA,Brereton HM

    更新日期:2008-07-31 00:00:00

  • Radioimmunoassay for immunoconglutinins.

    abstract::A competitive radioimmunoassay for immunoconglutinin (IK) was developed using isolated antostimulated rabbit IK labelled with 125I and glutaraldehyde-fixed sheep erythrocytes coated with fixed complement (EAC). IKs in test sera of human or animal origin produced specific inhibition of uptake of [125I]IK onto EAC and t...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Druguet M,Chayvialle JA,André C,Pepys MB

    更新日期:1980-01-01 00:00:00

  • Characterization of the diversity of T cell receptor γδ complementary determinant region 3 in human peripheral blood by Immune Repertoire Sequencing.

    abstract::γδ T cells function as sentinels in early host response to infections and malignancies. Although γδ T cells are regarded as innate immune cells and recognize antigens in a non-MHC restricted manner, they possess a huge diversity of complementary determinant region 3 (CDR3) of T cell receptor (TCR) generated by the rea...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Chen H,Zou M,Teng D,Zhang J,He W

    更新日期:2017-04-01 00:00:00

  • Two-colour combination enzyme-linked immunosorbent assay for the simultaneous detection of HBV and HIV infection.

    abstract::To reduce the cost, time and waste in screening for HIV and HBV infections a combined assay for HIV-1 and -2 antibodies and HBsAg has been developed. Monoclonal anti-HBs antibodies were co-immobilized with synthetic peptides representing immunodominant regions of HIV-1 and -2. The presence of anti-HIV antibodies in th...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Porstmann T,Nugel E,Henklein P,Döpel H,Rönspeck W,Pas P,von Baehr R

    更新日期:1993-01-14 00:00:00

  • Unbiased analysis, enrichment and purification of thymic stromal cells.

    abstract::The microenvironment of the thymus consists of functionally discrete niches composed of distinct stromal cell subsets. Clinically relevant changes affecting T-cell differentiation occur within these niches with age and injury caused by irradiation and chemotherapy treatments. The study of thymic stromal cells has been...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Gray DH,Fletcher AL,Hammett M,Seach N,Ueno T,Young LF,Barbuto J,Boyd RL,Chidgey AP

    更新日期:2008-01-01 00:00:00

  • Isolation of specific antibody under conditions of low ionic strength.

    abstract::Specific rabbit and goat antibodies to tobacco mosaic virus, bovine serum albumin and human gamma globulin have been isolated by acid dissociation from the antigen in the absence of salt. Maximum recovery of 96-100% of active antibody occurred at pH 2.8-2.9. The amount of antibody recovered within any given pH range w...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Hardie G,van Regenmortel MH

    更新日期:1977-01-01 00:00:00

  • Retransplantation of murine skin allografts: assessment of treatment effects on the allograft in the absence of effects on the host.

    abstract::In transplantation techniques previously described it was impossible to separate the effects of immunosuppressive treatments on the host from the effects directed at the allograft itself. The skin allograft retransplantation technique described here allows one to assess changes in the immunogenicity of the allograft g...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Sugarbaker PH,McCullough CS,Deutsch HL

    更新日期:1984-09-04 00:00:00

  • An immunofluorescence assay for complement activation by the classical pathway.

    abstract::The functional integrity of classical complement pathway components was determined by an immunofluorescence (IFL) assay based on the capacity of cytoskeletal intermediate filaments (IMF) to bind C1q and to activate the complement pathway. The assay uses IMF-rich capillary endothelium of human term placentae as complem...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Linder E,Rhen M,Meri S

    更新日期:1981-01-01 00:00:00

  • Use of ATPase-associated antigen (M2) for detection of antimitochondrial antibodies in primary biliary cirrhosis by fluorometric immunoassay.

    abstract::An indirect binding assay, the fluorometric immunoassay (FIAX), was established for the detection of anti-M2 antibodies which are specific markers for primary biliary cirrhosis (PBC). Submitochondrial particles (SMP) from beef heart and rat liver and the ATPase-associated antigen (M2) were used. The antigens were fixe...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Klein R,Lindenborn-Fotinos J,Berg PA

    更新日期:1983-11-11 00:00:00

  • The ethanol fractionation of mouse serum.

    abstract::A method for the ethanol fractionation of normal mouse serum has been developed which is rapid and simple to perform. The method was shown to be effective in purification of IgG1, IgG2 and gammaM-gammaA globulins. Gel filtration and zone electrophoresis proved to be effective in separating the gammaM-gammaA globulin c...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Measel JW,Cozad GC

    更新日期:1976-01-01 00:00:00

  • The simultaneous direct detection of Mycoplasma pneumoniae and Legionella pneumophila antigens in sputum specimens by a monoclonal antibody immunoblot assay.

    abstract::An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Madsen RD,Saeed FA,Coates SR

    更新日期:1987-11-05 00:00:00

  • The methodology for determining the efficacy of antibody-mediated immunity.

    abstract::The basic method for evaluating the efficacy of antibody-mediated immunity (AMI) dates from the 1890s and involves the administration of specific Ab to an immunologically naïve host followed by microbial or toxin challenge. Other methods used to evaluate AMI involve correlating the presence of specific Ab with resista...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审


    authors: Casadevall A

    更新日期:2004-08-01 00:00:00

  • Fc receptors on myeloid leukaemic cells: comparison between assays of rosette-forming cells.

    abstract::A comparison has been made between assays of Fc receptor bearing cells among blood leucocytes from normal individuals and from patients with myeloid leukaemia, using human anti-D coated erythrocytes (HEA) and sheep erythrocytes sensitised with a rabbit anti-sheep erythrocyte antibody (SEA). Differences were found betw...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Ridway JC,Taylor GM,Harris R

    更新日期:1979-01-01 00:00:00

  • Utility of Systematic Isolation of immune cell subsets from HIV-infected individuals for miRNA profiling.

    abstract:INTRODUCTION:Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bargalló ME,Guardo AC,Maleno MJ,Miralles L,Egaña-Gorroño L,Escribà T,García F,Gatell JM,Arnedo M,Plana M

    更新日期:2017-03-01 00:00:00

  • Cytokine and immunoglobulin concentrations in cervical secretions: reproducibility of the Weck-cel collection instrument and correlates of immune measures.

    abstract::Elucidation of local immune response at the cervix is important for understanding and evaluating STD vaccine approaches currently being proposed. However, no well-validated method exists for the collection of cervical secretions for evaluation of cervical immune response. The purpose of this study was to determine the...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Hildesheim A,McShane LM,Schiffman M,Bratti MC,Rodriguez AC,Herrero R,Morera LA,Cardenas F,Saxon L,Bowman FP,Crowley-Nowick PA

    更新日期:1999-05-27 00:00:00

  • Monopalmitic acid-peptide conjugates induce cytotoxic T cell responses against malarial epitopes: importance of spacer amino acids.

    abstract::Cytolytic T cells (CTL) play a critical role in providing protection against the liver stage of malaria infection. Previous investigations have shown that induction of CTL against peptide or proteins can be achieved by attachment of lipids. In the present study, we used the Plasmodium berghei circumsporozoite protein ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Verheul AF,Udhayakumar V,Jue DL,Wohlhueter RM,Lal AA

    更新日期:1995-06-09 00:00:00

  • An enzyme immunoassay for carcinoembryonic antigen (CEA) with homogeneous reactivity to different CEA preparations and low cross-reactivity with CEA-related normal antigens.

    abstract::A new sandwich-type solid-phase enzyme immunoassay (EIA) for carcinoembryonic antigen (CEA) was established by using two selected monoclonal antibodies (MAbs). One MAb, F82-61, which is immobilized on polystyrene beads, reacts with an epitope present on the N-terminal domain (N) of the CEA molecule and the other, F11-...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Nagasaki H,Watanabe S,Kuroki M,Matsumoto Y,Murakami M,Omoda N,Kondo N,Jinnai H,Takaoka H,Matsuoka Y

    更新日期:1993-06-18 00:00:00

  • Use of the periodate oxidation coupling method for the detection of antibody and antibody-producing cells specific for staphylococcal lipoteichoic acid.

    abstract::The periodate oxidation and chromium chloride coupling methods were compared for their ability to sensitize indicator erythrocytes with staphylococcal lipoteichoic acid (LTA) for the detection of specific antibody. Erythrocytes, sensitized with periodiate-activated lipoteichoic acid, were found to be superior for use ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Beining PR,Flannery GM,Caldes G,Prescott B,Baker PJ

    更新日期:1980-01-01 00:00:00

  • Difficulties in obtaining monoclonal antibodies to subsets of human leukocytes, using neonatal tolerance induction in mice.

    abstract::Many antigens are shared between different types of human leukocytes. In an effort to obtain new lineage-specific monoclonal antibodies, particularly antibodies to dendritic cells, we attempted to tolerize newborn mice to one type of leukocyte and then immunize the adults with another. We found that T cells, either un...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Yamaguchi Y,O'Doherty U,Peng M,Steinman RM

    更新日期:1995-04-12 00:00:00

  • Development of a continuous IL-7-dependent murine pre-B cell line PB-1 suitable for the biological characterisation and assay of human IL-7.

    abstract::Human interleukin-7 (IL-7) is a cytokine that appears to be critical for early T- and B-cell development and although IL-7 is currently under investigation as a therapeutic agent in a variety of hematolymphopoietic disorders, there have been few instances of the detection or investigation of this cytokine using a biol...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Mire-Sluis AR,Healey L,Griffiths S,Hockley D,Thorpe R

    更新日期:2000-03-06 00:00:00

  • The in vitro production of chimeric murine thymus from nonlymphoid embryonic precursors.

    abstract::A simplified technique for the production of chimeric thymus completely in vitro from the third pharyngeal pouch (TPP) of 10-day mouse embryos has been examined. The results are similar to those obtained by a more cumbersome technique published by others. This modified technique permits the production of large numbers...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Pyke KW,Bartlett PF,Mandel TE

    更新日期:1983-03-11 00:00:00

  • Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy.

    abstract::Near-infrared Raman spectroscopy may provide a highly sensitive, noninvasive means to identify activation status of leukocytes. The purpose of the current study was to establish Raman spectroscopic characteristics of T cell activation. Activation of the RsL.11 T cell clone in vitro with Con A resulted in specific decr...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Mannie MD,McConnell TJ,Xie C,Li YQ

    更新日期:2005-02-01 00:00:00

  • An agglutination assay using emulsified oils.

    abstract::Dispersed fluorocarbon oil droplets were used as the antigen-carrier particle in an immunological agglutination assay. The extent of agglutination was found to vary with the percentage of antigen to that of inert protein used in the solution to coat and stabilize the oil droplets. Maximum sensitivity was observed when...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Prather TL,Grande J,Keese CR,Giaever I

    更新日期:1986-03-13 00:00:00

  • Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen.

    abstract::Establishment of humoral immunity against pathogens is dependent on events that occur in the germinal center and the subsequent induction of high-affinity neutralizing antibodies. Quantitative assays that allow monitoring of affinity maturation and duration of antibody responses can provide useful information regardin...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Lynch HE,Stewart SM,Kepler TB,Sempowski GD,Alam SM

    更新日期:2014-02-01 00:00:00

  • Performance evaluation of the MBio Diagnostics point-of-care CD4 counter.

    abstract::The measurement of the absolute CD4 T-cell count is critical in the initial evaluation and staging of HIV-infected persons, yet access to this technology remains limited in many low resource settings where disease burden is highest. Here we evaluate the performance of a prototype point-of-care device (POC) to quantify...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Logan C,Givens M,Ives JT,Delaney M,Lochhead MJ,Schooley RT,Benson CA

    更新日期:2013-01-31 00:00:00