Abstract:
:We studied reactivity of highly purified pituitary hormones in our human calcitonin (hCT) radioimmunoassay (RIA) which can detect 1 pg of hCT. ACTH at doses of greater than 1 microgram of peptide per RIA tube reacted in the hCT assay, as did beta-endorphin (beta EPH) at a dose of 10 micrograms per tube. No reactivity was observed with comparable concentrations of all other known pituitary hormones. ACTH also reacted at doses greater than 1 microgram per tube with 7 other hCT antisera which recognized differing antigenic determinants in the calcitonin molecule but it was not reactive with 2 antisera against porcine calcitonin or 2 antisera against salmon calcitonin. This slight degree of cross-reactivity of hACTH and beta EPH in the hCT RIA cannot account for the presence of immunoreactive CT in pituitary glands. Nevertheless, antisera used for the localization of peptides must be rigorously tested for the existence of cross-reactivities with other possible substances, especially if such antisera detect the peptide in unexpected tissues.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Catherwood BD,Deftos LJdoi
10.1016/0022-1759(79)90144-3subject
Has Abstractpub_date
1979-01-01 00:00:00pages
315-22issue
3-4eissn
0022-1759issn
1872-7905pii
0022-1759(79)90144-3journal_volume
31pub_type
杂志文章abstract::The discovery of a naturally occurring missense point mutation in the gene encoding Fas-ligand (FasL/CD95L) in generalized lymphoproliferative disease (gld) mice has lead to the characterization of FasL as an important mediator of apoptosis. Further analysis of FasL function can be facilitated by crossing the gld muta...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(85)90231-5
更新日期:1985-09-03 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90211-b
更新日期:1992-09-18 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(88)90452-8
更新日期:1988-04-22 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1983-05-27 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1984-05-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90208-b
更新日期:1992-09-18 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90264-x
更新日期:1994-03-10 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(78)90177-1
更新日期:1978-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(97)90008-9
更新日期:1977-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2003.12.014
更新日期:2004-05-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90087-a
更新日期:1992-07-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2012.02.009
更新日期:2012-04-30 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(02)00329-0
更新日期:2002-12-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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更新日期:2015-07-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90313-2
更新日期:1979-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(77)90121-1
更新日期:1977-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(85)90312-6
更新日期:1985-02-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90186-w
更新日期:1992-10-02 00:00:00
abstract::Antibody-based sensors are now widely used in therapeutics, diagnostics, and in environmental monitoring. Recombinant antibodies are becoming integral parts of such devices due to their reported high affinities, their capacity for engineering to achieve highly defined performance characteristics and the fact that thei...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2018.10.005
更新日期:2018-12-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
doi:10.1016/s0022-1759(99)00143-x
更新日期:1999-12-10 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(02)00018-2
更新日期:2002-04-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2010.04.005
更新日期:2010-06-30 00:00:00
abstract::A new method is described for demonstrating the individual subclasses of human IgG immunoglobulins by means of analytical isotachophoresis. Individual subclasses of IgG purified from sera of multiple myeloma patients have distinct mobility patterns. When a mixture with purified IgG for each of the 4 subclasses is anal...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90163-7
更新日期:1979-01-01 00:00:00