Recombinant fusion proteins for haemagglutination-based rapid detection of antibodies to HIV in whole blood.

abstract：:Cephalexin was structurally modified by the attachment of a spacer at the carboxylic acid through which it was subsequently covalently attached to BSA. This method permitted the molecule to be attached without cleavage of the beta-lactam ring giving a conjugate distinct from previously described immunogenic preparatio...

journal_title：Journal of immunological methods

authors： Kachab EH,Wu WY,Chapman CB

更新日期：1992-02-14 00:00:00

abstract：:This paper reports a new ELISA to measure the level of rabies anti-glycoprotein G antibodies after vaccination. The Platelia Rabies II kit was evaluated on different populations of dogs, cats and foxes. For each target species, sera from naive, unvaccinated and vaccinated animals were tested. Platelia Rabies II result...

journal_title：Journal of immunological methods

authors： Servat A,Feyssaguet M,Blanchard I,Morize JL,Schereffer JL,Boue F,Cliquet F

更新日期：2007-01-10 00:00:00

abstract：:Counting of rat mast cells and assessment of degranulation were performed by flow-cytometry using a cytograph. It was shown that the number of mast cells in a total cell population may be obtained rapidly and reproducibly following toluidine blue staining. A dose-response for degranulation by compound 48/80 was perfor...

journal_title：Journal of immunological methods

authors： Nakagawa T,Stadler BM,De Weck AL

更新日期：1980-01-01 00:00:00

abstract：:Concanavalin A-activated murine suppressor T cells act via the mediator, soluble immune response suppressor (SIRS) which non-specifically suppresses IgM and IgG antibody responses to a variety of antigens, cytotoxic T lymphocyte responses and proliferative responses to alloantigens and mitogens in vitro. SIRS is a pro...

journal_title：Journal of immunological methods

authors： Aune TM,Pierce CW

更新日期：1982-08-27 00:00:00

abstract：:To select sequences complementary to their binding sites, two anti-streptokinase (SK) monoclonal antibodies (mAbs), A4.5 and A5.5, were used in biopanning of 15-mer and hexamer phage-displayed peptide libraries, respectively. mAb A4.5 inhibits the catalytic activity of streptokinase-plasminogen activator complex (SKPA...

journal_title：Journal of immunological methods

authors： Parhami-Seren B,Krudysz J,Tsantili P

更新日期：2002-09-15 00:00:00

abstract：:An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

journal_title：Journal of immunological methods

authors： Madsen RD,Saeed FA,Coates SR

更新日期：1987-11-05 00:00:00

abstract：:ADP-ribosylation factors (Arf) are small GTP-binding proteins involved in vesicular transport and the activation of phospholipase D (PLD). The conversion of Arf-GDP to Arf-GTP is promoted in vivo by guanine nucleotide exchange factors such as ARNO or cytohesin-1. In order to examine the expression of ARNO and cytohesi...

journal_title：Journal of immunological methods

authors： Garceau V,Houle MG,Chouinard F,Gagnon S,Harbour D,Naccache PH,Bourgoin SG

更新日期：2001-03-01 00:00:00

abstract：:Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC...

journal_title：Journal of immunological methods

authors： Joshi L,Ponnana M,Sivangala R,Chelluri LK,Nallari P,Valluri VL,Gaddam S

更新日期：2016-05-01 00:00:00

abstract：:Proper evaluation of immunogenicity during clinical development of biotherapeutics is a major challenge to bioanalytical scientists, in part due to matrix interference in anti-drug antibody (ADA) and neutralizing antibody (NAB) assays. If not addressed, matrix interference could confound the immunogenicity assessment ...

journal_title：Journal of immunological methods

authors： Wang Y,Luong M,Guadiz C,Zhang M,Gorovits B

更新日期：2019-11-01 00:00:00

abstract：:Helicobacter pylori is a bacterial pathogen, estimated to infect half the world's population. The bacterium is the aetiological cause of gastritis, the common precursor for peptic ulcer disease and gastric cancer. Immunisation of at-risk individuals is the most cost-effective means of dealing with such a widespread pa...

journal_title：Journal of immunological methods

authors： Keenan JI,Allardyce RA,Bagshaw PF

更新日期：1997-11-10 00:00:00

abstract：:The existing methods for phagocytosis evaluation are inadequate for assessing all the real events occurring during phagocytosis, or for continuously following the kinetics of the process. Our purpose is to establish the use of luminous bacteria as an object for phagocytosis. The bioluminescence test offers an easy and...

journal_title：Journal of immunological methods

authors： Barak M,Ulitzur S,Merzbach D

更新日期：1983-11-25 00:00:00

abstract：:A new system for assaying monoclonal antibodies consisting of an 8 x 12 array of sticks which fits into a 96-well microtiter plate is described. Tests using virus specific monoclonal antibodies and virus proteins demonstrated sensitivity equivalent to the conventional microtiter plate assay. Antibody production, antig...

journal_title：Journal of immunological methods

authors： Scheinberg DA,Pan XQ,Wilsnack R,Strand M

更新日期：1983-03-25 00:00:00

abstract：:This paper describes an improved method for detecting tyrosine phosphorylation levels in T cell subsets by flow cytometry early after CD3 crosslinking stimulation. It consists in introducing gentle paraformaldehyde fixation between CD3 crosslinking in cold conditions and the shift to 37 degrees C, which activates down...

journal_title：Journal of immunological methods

authors： Vuillier F,Scott-Algara D,Cayota A,Siciliano J,Nugeyre MT,Dighiero G

更新日期：1995-09-11 00:00:00

abstract：:To increase the selection efficiency and productivity of stable clones expressing recombinant antibody-IL-2 fusion protein, a dicistronic expression vector containing the anti-erbB2 scFv-Fc-IL-2 fusion gene followed by a weakened neo gene, was constructed to allow for the concurrent translation of the recombinant prot...

journal_title：Journal of immunological methods

authors： Chen L,Xie Z,Teng Y,Wang M,Shi M,Qian L,Hu M,Feng J,Yang X,Shen B,Guo N

更新日期：2004-12-01 00:00:00

abstract：:A method is described for the purification of monoclonal antibody from mouse ascitic fluid. The fluid is clarified and the lipid removed using silicon dioxide powder, before the immunoglobulin is precipitated using polyethylene glycol. The method provides IgM antibody in high yield and good purity. In the case of IgG ...

journal_title：Journal of immunological methods

authors： Neoh SH,Gordon C,Potter A,Zola H

更新日期：1986-07-24 00:00:00

abstract：:An immunoaffinity method for the purification of hepatitis B surface antigen (HBsAg) using monoclonal anti-idiotype (anti-Id) as a specific eluent is described. Ammonium sulfate fractions of HBsAg-containing plasma were absorbed on monoclonal idiotype (Id) immunoadsorbents. The bound material was eluted from the colum...

journal_title：Journal of immunological methods

authors： Kim WB,Kim BM,Choi EC,Kim BK

更新日期：1990-01-24 00:00:00

abstract：:Laurell crossed immunoelectrophoresis (two-dimensional electroimmunodiffusion) was used to prepare minute amounts of purified parasite antigens complexed with their precipitating antibodies obtained from rabbits. These complexes, emuslified in Freund's complete adjuvant, were then used to prime rabbits for selective p...

journal_title：Journal of immunological methods

authors： Hillyer GV,Cervoni M

更新日期：1978-01-01 00:00:00

abstract：:Rabbit antiserum, specific for protein A from Staphylococcus aureus, was conjugated to alkaline phosphatase and used in a double antibody solid-phase enzyme immunoassay. The assay was developed to monitor eluate from a large-scale protein A-Sepharose affinity column used to purify monoclonal antibodies for human clini...

journal_title：Journal of immunological methods

authors： Dertzbaugh MT,Flickinger MC,Lebherz WB 3rd

更新日期：1985-10-24 00:00:00

abstract：:Thrombospondin (TSP), a platelet glycoprotein, was purified from platelet supernatants applied sequentially to Sepharose 4B and heparin-agarose affinity columns. This TSP inhibited alternative pathway activity in serum as assessed by lysis of rabbit erythrocytes and contained bound heparin, a substance which is known ...

journal_title：Journal of immunological methods

authors： Scott PE,Purkall DB,Ruddy S

更新日期：1997-03-28 00:00:00

abstract：:Frequencies of lipopolysaccharide (LPS)-reactive B cells in the mouse can be determined in the limiting dilution culture system developed by Andersson et al. (1976, 1977a) which is completely dependent upon the presence of thymus filler cells, usually of rat origin. The assessment of B cell clones of mouse origin, how...

journal_title：Journal of immunological methods

authors： Hooijkaas H,Preesman AA,Benner R

更新日期：1982-01-01 00:00:00

abstract：:E. coli O157:H7 is a pathogenic microorganism that has been implicated in numerous cases of foodborne illnesses. A variety of rapid methods exist that show promise for the presumptive detection of this pathogen without the immediate need for incubating test samples for hours to days in microbial enrichment and culture...

journal_title：Journal of immunological methods

authors： Gehring AG,Irwin PL,Reed SA,Tu SI,Andreotti PE,Akhavan-Tafti H,Handley RS

更新日期：2004-10-01 00:00:00

abstract：:Incorporation of control valves into a previously described device enabled us to regulate the formation of 8 suction blisters on the upper surface of the forearm in adult human volunteers. After the removal of the raised epidermis and blister fluid, uniform areas of denuded dermis were obtained by placing hollow adhes...

journal_title：Journal of immunological methods

authors： Fernandes AC,Anderson R,Ras GJ

更新日期：1985-11-07 00:00:00

abstract：:A simple method to study 19S anti-SRBC antibody production is described. This technique, performed by incubation of immune spleen cells, SRBC and C in the fluid phase, utilises photomeric estimation of antibody mediated haemoglobin release. The test is quicker and more accurate than the two-step haemolytic plaque assa...

journal_title：Journal of immunological methods

authors： Van Dijk H,Bloksma N

更新日期：1977-01-01 00:00:00

abstract：:A comparison has been made between assays of Fc receptor bearing cells among blood leucocytes from normal individuals and from patients with myeloid leukaemia, using human anti-D coated erythrocytes (HEA) and sheep erythrocytes sensitised with a rabbit anti-sheep erythrocyte antibody (SEA). Differences were found betw...

journal_title：Journal of immunological methods

authors： Ridway JC,Taylor GM,Harris R

更新日期：1979-01-01 00:00:00

abstract：:Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation ...

journal_title：Journal of immunological methods

authors： Miyakawa S,Okui T,Shiraishi T,Yoshihara T,Hirayama M,Satomi Y,Hamada T,Nishida M,Akimoto C,Sato S

更新日期：2020-05-01 00:00:00

abstract：:alpha-Lactalbumin has been purified from mouse milk by the use of ammonium sulfate precipitation, Bio-Gel P-100 chromatography and DEAE-cellulose chromatography. Mouse alpha-lactalbumin exists as two major charge forms with the same molecular weight. Both charge forms have been used in the development of a radioimmuno...

journal_title：Journal of immunological methods

authors： Zamierowski MM,Ebner KE

更新日期：1980-01-01 00:00:00

abstract：:IgM antibodies are often of low affinity (dissociation constant (Kd) > 10(-5) M) and therefore they are usually neglected as tools in, e.g., immunoassays. Previous studies have shown that low affinity biological interactions can be studied and exploited in affinity chromatography, biosensor technology and capillary el...

journal_title：Journal of immunological methods

authors： Strandh M,Ohlin M,Borrebaeck CA,Ohlson S

更新日期：1998-05-01 00:00:00

abstract：:Western blotting is one of the most commonly and widely used methods for identifying the presence of an antigen in biochemical studies. The present study describes a novel 'coupled blotting' approach that allows antigen on dot and Western blots to be simultaneously probed with primary and secondary probing reagents. T...

journal_title：Journal of immunological methods

authors： Sundaram P

更新日期：1996-09-09 00:00:00

abstract：:Melanins are notoriously difficult to work with because of their unique physical and chemical properties. The study of melanins is hampered by the scarcity of melanin-specific reagents and serological techniques. In this study we describe modifications to the standard method for the isolation of melanins from in vitro...

journal_title：Journal of immunological methods

authors： Rosas AL,Nosanchuk JD,Gómez BL,Edens WA,Henson JM,Casadevall A

更新日期：2000-10-20 00:00:00

abstract：:A method is described to quantitate complement fragment C4ai in human plasma, synovial fluid, and urine. Samples are first precipitated with 50% saturated (NH4)2SO4 to remove cross-reactive macromolecules C4 and pro-C4. Whereas greater than 97% of C4 is removed by this precipitation step, 88% of C4ai remains in soluti...

journal_title：Journal of immunological methods

authors： Gorski JP

更新日期：1981-01-01 00:00:00