Rapid screening of monoclonal antibodies: new 'microstick' radioimmunoassay.

abstract：:The ability to rapidly screen a complex pathogen proteome for proteins that elicit recall T-lymphocyte responses from immune individuals would accelerate vaccine development. An outer membrane fraction of the rickettsial pathogen Anaplasma marginale induces protective immunity against infection and disease in cattle. ...

journal_title：Journal of immunological methods

authors： Lopez JE,Beare PA,Heinzen RA,Norimine J,Lahmers KK,Palmer GH,Brown WC

更新日期：2008-03-20 00:00:00

abstract：:To increase the selection efficiency and productivity of stable clones expressing recombinant antibody-IL-2 fusion protein, a dicistronic expression vector containing the anti-erbB2 scFv-Fc-IL-2 fusion gene followed by a weakened neo gene, was constructed to allow for the concurrent translation of the recombinant prot...

journal_title：Journal of immunological methods

authors： Chen L,Xie Z,Teng Y,Wang M,Shi M,Qian L,Hu M,Feng J,Yang X,Shen B,Guo N

更新日期：2004-12-01 00:00:00

abstract：:Human interleukin 2 (IL-2) was radioiodinated by a modified form of the chloramine-T reaction. Comparison with biosynthetically radiolabeled IL-2 demonstrated that the iodinated molecule retained similar receptor binding characteristics and proliferation-inducing ability. The iodinated molecule also possessed the dist...

journal_title：Journal of immunological methods

authors： Robb RJ,Mayer PC,Garlick R

更新日期：1985-07-16 00:00:00

abstract：:The method of extraction of outer membrane proteins (OMPs), the conditions of electrophoretic transfer, and the conditions of antibody binding, were compared in Western blotting studies of Neisseria meningitidis outer membrane antigens. The OMP profiles obtained by SDS-PAGE of outer membrane vesicles extracted with li...

journal_title：Journal of immunological methods

authors： Davies RL,Wall RA,Borriello SP

更新日期：1990-12-05 00:00:00

abstract：:Monoclonal antibodies can now be generated against a wide variety of antigens. However, a potentially useful monoclonal antibody may be of the wrong class or subclass for a particular task. Antibodies of the desired class can be obtained by identifying rare subclones in which the variable region has been rearranged to...

journal_title：Journal of immunological methods

authors： Spira G,Bargellesi A,Teillaud JL,Scharff MD

更新日期：1984-11-30 00:00:00

abstract：:Dispersed fluorocarbon oil droplets were used as the antigen-carrier particle in an immunological agglutination assay. The extent of agglutination was found to vary with the percentage of antigen to that of inert protein used in the solution to coat and stabilize the oil droplets. Maximum sensitivity was observed when...

journal_title：Journal of immunological methods

authors： Prather TL,Grande J,Keese CR,Giaever I

更新日期：1986-03-13 00:00:00

abstract：:Six monoclonal and two polyclonal antibodies to fluorescein (FLU) were affinity purified and immobilized on Immulon 2 polystyrene as capture antibodies (CAbs): (a) by passive adsorption at pH 9.6, (b) via a streptavidin bridge to a biotinylated carrier molecule, and (c) via an antiglobulin which had been previously ad...

journal_title：Journal of immunological methods

authors： Butler JE,Ni L,Nessler R,Joshi KS,Suter M,Rosenberg B,Chang J,Brown WR,Cantarero LA

更新日期：1992-06-24 00:00:00

abstract：:A small scale solid-phase radioimmunoassay employing 125I-labelled protein A is described which is suitable for screening large numbers of monoclonal antibodies directed against antigens which can be prepared in small amounts only, for example oncoviral proteins. The use of polystyrene Terasaki microtest plates instea...

journal_title：Journal of immunological methods

authors： Tanaka T,Kurth R

更新日期：1984-12-31 00:00:00

abstract：:We present a double antibody immunoassay for tumour necrosis factor alpha (TNF alpha) with a peroxidase dependent endpoint which can be detected by absorbance or chemiluminescence depending on the choice of substrate. The chemilumimetric and colorimetric assays have a detection threshold in human serum of 3.9 pg/ml an...

journal_title：Journal of immunological methods

authors： Lamb WR,Pumphrey RS,Brenchley PE

更新日期：1992-11-05 00:00:00

abstract：:The optimal conditions for the detection of outer membrane proteins (OMPs) and lipopolysaccharide (LPS) of Pasteurella haemolytica by immunoblotting were evaluated. The variables examined included the equilibration time of the gels before transfer, composition of the transfer buffer, type of blotting membrane, blockin...

journal_title：Journal of immunological methods

authors： Davies RL,Parton R,Coote JG,Gibbs HA,Freer JH

更新日期：1994-01-03 00:00:00

abstract：:An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

journal_title：Journal of immunological methods

authors： Madsen RD,Saeed FA,Coates SR

更新日期：1987-11-05 00:00:00

abstract：:Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not di...

journal_title：Journal of immunological methods

authors： Kajdacsy-Balla AA,Mendes NF

更新日期：1976-01-01 00:00:00

abstract：:An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange s...

journal_title：Journal of immunological methods

authors： Luk JM,Lindberg AA

更新日期：1991-03-01 00:00:00

abstract：:We evaluated an enzyme-linked immunosorbent assay (ELISA) for antibodies to native DNA (nDNA) in which protamine was used to link DNA to polystyrene. Elevated anti-nDNA was largely restricted to patients with systemic lupus erythematosus (SLE), and within this group good correlation between ELISA and the ammonium sulf...

journal_title：Journal of immunological methods

authors： Rubin RL,Joslin FG,Tan EM

更新日期：1983-10-28 00:00:00

abstract：:Immune complexes attached to and ingested by human polymorphonuclear (PMN) cells were quantified by cytofluorometry using a fluorescence quenching assay which permits differentiation between attachment and ingestion. The fluorescence intensity decreased after ingestion as a result of the low pH in the phagolysosomes. ...

journal_title：Journal of immunological methods

authors： Sahlin S,Hed J,Rundquist I

更新日期：1983-05-27 00:00:00

abstract：:We have used a conceptually novel way to construct antibody mimics based on the binding of a noncatalytic enzyme to its substrate. Bacteriophage-derived endosialidase cleaves polysialic acid (polySia), an important oncofetal and bacterial antigen, which is poorly immunogenic. We fused to green fluorescent protein (GFP...

journal_title：Journal of immunological methods

authors： Jokilammi A,Ollikka P,Korja M,Jakobsson E,Loimaranta V,Haataja S,Hirvonen H,Finne J

更新日期：2004-12-01 00:00:00

abstract：:In this study we have employed surface plasmon resonance to examine the kinetic binding constants of a new class of soluble cytokine receptor complexes. The solution assembly of both homomeric and heteromeric interleukin-2 receptor ectodomain complexes has been achieved using coiled-coil molecular recognition. These c...

journal_title：Journal of immunological methods

authors： Wu Z,Johnson KW,Ciardelli TL

更新日期：1995-06-14 00:00:00

abstract：:A new T(H1)/T(H2) in vitro model for mechanistic studies and drug screening in human T cells was established working with ficoll-separated PBMCs or elutriated lymphocytes cultured in serum-free medium. Human T cells could be kept viable and reactive in this medium for several months. In this model, superantigens (SAs)...

journal_title：Journal of immunological methods

authors： Gehring S,Schlaak M,van der Bosch J

更新日期：1998-10-01 00:00:00

abstract：:Successful delivery of effector cells to tumor vasculature and the expression of cellular activities, such as extravasation and tumor infiltration are important aspects of the immune intervention of neoplastic disease. The structural rigidity of the effector cell, an indication of its cytoskeletal characteristics, pla...

journal_title：Journal of immunological methods

authors： Melder RJ,Jain RK

更新日期：1994-09-30 00:00:00

abstract：:Nonspecific effects of reagents used to selectively isolate lymphocyte subpopulations are most often analyzed according to their influence on cell viability and proliferation. While characterizing monoclonal anti-human B cell antibodies, we detected consistent changes in B cell function between individual donors, no m...

journal_title：Journal of immunological methods

authors： Munchus MS,Levitt D

更新日期：1984-02-10 00:00:00

abstract：:A new quantitative assay for migration inhibitory factor (MIF) employs 3H-labelled cultured mouse or human lymphoid cells migrating from capillary tubes. Capillaries filled with labelled cells are placed in liquid scintillation counting vials, along with the MIF-containing sample and are removed at the end of a five-h...

journal_title：Journal of immunological methods

authors： McDaniel MC,Robbins CH,Hokanson JA,Papermaster BW

更新日期：1978-01-01 00:00:00

abstract：:Using human heart-type fatty acid-binding protein (H-FABP) as an early cardiac marker to confirm or exclude a diagnosis of acute myocardial infarction (AMI) soon after admission requires a rapid assay system. Due to the requirement of skillful technicians and complicated assay procedures, most immunochemical assays fo...

journal_title：Journal of immunological methods

authors： Chan CP,Sum KW,Cheung KY,Glatz JF,Sanderson JE,Hempel A,Lehmann M,Renneberg I,Renneberg R

更新日期：2003-08-01 00:00:00

abstract：:Antibody molecules bind to antigen with six complementary determining region (CDR) loops, three of which are located on each variable heavy (V(H)) and light (V(L)) chains. Discovery and optimization of antibodies that bind antigen using in vitro techniques require diversification of one or more of these CDRs. Since an...

journal_title：Journal of immunological methods

authors： Leonard B,Sharma V,Smider V

更新日期：2006-12-20 00:00:00

abstract：:A new sandwich immunoassay method for measuring human C5a-des-Arg was developed using monoclonal antibody specifically reactive with C5a-des-Arg. Monoclonal antibodies were obtained from a panel of hybridomas produced by fusion of mouse myeloma cells, P3 X 63-AG8,653, with spleen cells from a CBF1(C57BL/6 X BALB/c) mo...

journal_title：Journal of immunological methods

authors： Takeda J,Kinoshita T,Takata Y,Kozono H,Tanaka E,Hong K,Inoue K

更新日期：1987-08-03 00:00:00

abstract：:Hybridomas secreting monoclonal antibodies (MAbs) against African horse sickness virus (AHSV) were generated using different AHSV antigen preparations (inactivated AHSV, semi-purified virus, and a preparation of nonstructural viral proteins) in one of three different in vitro primary immunization systems: (i) the Cel-...

journal_title：Journal of immunological methods

authors： Halabi G,McCullough KC

更新日期：1995-10-26 00:00:00

abstract：:Humans exposed to rabies virus must be promptly treated by passive immunization with anti-rabies antibody and active immunization with rabies vaccine. Currently, antibody prepared from pooled human serum or from immunized horses is utilized. However, neither of these reagents are readily available, entirely safe, or c...

journal_title：Journal of immunological methods

authors： Morimoto K,Schnell MJ,Pulmanausahakul R,McGettigan JP,Foley HD,Faber M,Hooper DC,Dietzschold B

更新日期：2001-06-01 00:00:00

abstract：:Flow cytometry offers a promising alternative to the current methods of amniocentesis or chorionic villus sampling (CVS) for fetal cell sorting for prenatal diagnosis. While flow cytometric methods have been greatly improved to be more sensitive at detecting fetal cells within the maternal circulation, there are still...

journal_title：Journal of immunological methods

authors： Curtis MG,Walker B,Denny TN

更新日期：2011-01-05 00:00:00

abstract：:We have recently described a new colorimetric DNA enzyme immunoassay (DEIA) for detecting specific hybrids of complementary nucleic acids. This technology is based on an antibody that selectively recognizes double, but not single stranded DNA and therefore reveals the hybridization event independently from the DNA seq...

journal_title：Journal of immunological methods

authors： Bettinardi A,Imberti L,Sottini A,Primi D

更新日期：1992-01-21 00:00:00

abstract：:In this paper we provide both a theoretical and experimental analysis of the sensitivity of a DNA quantitation assay using a fluorescent chromophore which non-covalently binds dsDNA. It is well-known that the range of DNA concentrations available for fluorescence quantitation depends on the concentration of the chromo...

journal_title：Journal of immunological methods

authors： Dragan AI,Bishop ES,Casas-Finet JR,Strouse RJ,Schenerman MA,Geddes CD

更新日期：2010-10-31 00:00:00

abstract：:The therapeutic use of dendritic cells (DC) in antigen-specific anti-tumor vaccines, requires sufficient numbers of functional DC, the preparation of which should comply with the code of Good Manufacturing Practice. In addition, the expression of tumor specific antigen should be possible in these DC. As a preclinical ...

journal_title：Journal of immunological methods

authors： Rouard H,Léon A,Klonjkowski B,Marquet J,Tennezé L,Plonquet A,Agrawal SG,Abastado JP,Eloit M,Farcet JP,Delfau-Larue MH

更新日期：2000-07-31 00:00:00