Abstract：
:A new sandwich immunoassay method for measuring human C5a-des-Arg was developed using monoclonal antibody specifically reactive with C5a-des-Arg. Monoclonal antibodies were obtained from a panel of hybridomas produced by fusion of mouse myeloma cells, P3 X 63-AG8,653, with spleen cells from a CBF1(C57BL/6 X BALB/c) mouse immunized with purified C5a. The reactivities of these monoclonal antibodies against C5a, C5a-des-Arg and C5 were tested by solid-phase radioimmunoassay. One of the antibodies reacted with C5a-des-Arg, but not with C5a and C5. By use of this antibody for capturing antibody in sandwich immunoassay, a rapid and simple method was developed for measuring C5a-des-Arg without previous removal of C5. The sensitivity of this assay system was approximately 1 ng/ml for C5a-des-Arg.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Takeda J,Kinoshita T,Takata Y,Kozono H,Tanaka E,Hong K,Inoue Kdoi
10.1016/0022-1759(87)90159-1subject
Has Abstractpub_date
1987-08-03 00:00:00pages
265-70issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(87)90159-1journal_volume
101pub_type
杂志文章abstract：:Little is known about the correlation between the amount of anti-A antibodies and the respective complement activating capacity. We have therefore developed a new ELISA procedure for the study of complement activation by anti-A antibodies bound to immobilized blood group substance A through the classical pathway. C1q ...
journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/0022-1759(88)90181-0
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1983-09-30 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1999-02-01 00:00:00
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journal_title：Journal of immunological methods
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