An automated device for immunocytochemistry.

abstract：:A new T(H1)/T(H2) in vitro model for mechanistic studies and drug screening in human T cells was established working with ficoll-separated PBMCs or elutriated lymphocytes cultured in serum-free medium. Human T cells could be kept viable and reactive in this medium for several months. In this model, superantigens (SAs)...

journal_title：Journal of immunological methods

authors： Gehring S,Schlaak M,van der Bosch J

更新日期：1998-10-01 00:00:00

abstract：:Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based o...

journal_title：Journal of immunological methods

authors： Houthuys E,Movahedi K,De Baetselier P,Van Ginderachter JA,Brouckaert P

更新日期：2010-07-31 00:00:00

abstract：:We have developed an enzyme-linked immunosorbent assay (ELISA) for the quantitation of C4 nephritic factor (C4NeF). Incubation of the C4NeF-positive serum from patient M.I. with normal human serum (NHS) in the presence of human aggregated IgG (AHG) resulted in the formation of stable C4-C2 complex. No complex was form...

journal_title：Journal of immunological methods

authors： Seino J,Kinoshita Y,Sudo K,Horigome I,Sato H,Narita M,Noshiro H,Kudo K,Fukuda K,Saito T

更新日期：1990-03-27 00:00:00

abstract：:Using a solid-phase non-radioisotopic (non-RI) reverse transcriptase (RT) assay, antibodies inhibiting human immunodeficiency virus type 1 (HIV-1) RT activity (RTI antibody) were investigated for their ability to inhibit binding of RT to a template-primer and DNA polymerization. The RTI antibody inhibited the binding ...

journal_title：Journal of immunological methods

authors： Odawara F,Sano K,Otake T,Okubo S,Nakano T,Takasaki T,Ueba N,Misaki H,Nakai M

更新日期：1996-12-15 00:00:00

abstract：:A simple semi-automated method for the assay of large numbers of replicate microcultures for the presence of antibody-forming cell clones is described. The supernatant medium is removed from microcultures by a single sharp flick on inverting the tray. The cultured cells are mixed with 0.05 ml of a plaque-revealing mix...

journal_title：Journal of immunological methods

authors： Pike BL,Jennings G,Shortman K

更新日期：1982-01-01 00:00:00

abstract：:This report describes a simple solid-phase technique for the positive selection of lymphocytes labeled with fluoresceinated antibodies. B lymphocytes were labeled with fluoresceinated anti-human Ig or monoclonal anti-human Ia (L243), and then were bound to plastic culture dishes coated with affinity purified goat anti...

journal_title：Journal of immunological methods

authors： Fong S,Tsoukas CD,Pasquali JL,Fox RI,Rose JE,Raiklen D,Carson DA,Vaughan JH

更新日期：1981-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for screening production of monoclonal antibodies with specificity for HBsAg. Mouse hybridoma IgG were firstly extracted from assay medium with goat anti-mouse IgG adsorbed on polystyrene beads. The specific antibody was revealed by saturation with HBs antigen...

journal_title：Journal of immunological methods

authors： Boniolo A,Dovis M,Matteja R

更新日期：1982-01-01 00:00:00

abstract：:Leukocyte scintigraphy has been used as a standard diagnostic procedure for the detection of inflammation in vivo. In this study, we developed a method of labelling purified lymphocytes with technetium99m-hexamethyl propyleneamine oxime (Tc99m-HMPAO) without significantly impairing their function. This was confirmed b...

journal_title：Journal of immunological methods

authors： Jaakkola K,Knuuti J,Söderlund K,Saraste A,Jalkanen S,Voipio-Pulkki LM

更新日期：1998-05-01 00:00:00

abstract：:A dot-ELISA technique for the detection of G. lamblia specific antigen in stool eluates of clinical cases of giardiasis was developed and evaluated employing monospecific antibodies to a G. lamblia specific coproantigen with a molecular mass of 66 kDa. The assay detected 22 (91.7%) of the 24 microscopically confirmed ...

journal_title：Journal of immunological methods

authors： Vinayak VK,Dutt P,Puri M

更新日期：1991-03-21 00:00:00

abstract：:Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was...

journal_title：Journal of immunological methods

authors： Zhao J,Chang W,Liu L,Xing X,Zhang C,Meng H,Gopinath SCB,Lakshmipriya T,Chen Y,Liu Y

更新日期：2021-02-01 00:00:00

abstract：:Ranibizumab (Lucentis®), a humanized antigen-binding fragment (Fab) monoclonal antibody that blocks VEGF-A activity, is currently approved for the treatment of several retinal degenerative diseases. The assessment of drug pharmacokinetics (PK) is essential for evaluating exposure as it relates to drug safety and effic...

journal_title：Journal of immunological methods

authors： Lowe J,Wakshull E,Shek T,Chuntharapai A,Elliott R,Rusit J,Maia M

更新日期：2018-10-01 00:00:00

abstract：:Genetic mapping of differentiation specific surface antigens has been hampered by difficulty in preparation of interspecies hybrid cells which continue to express differentiated functions. A method has been developed for production of interspecies T cell hybrids which continue to express T cell specific cell surface m...

journal_title：Journal of immunological methods

authors： Messer Peters PG,Kamarck ME,Ruddle FH

更新日期：1983-08-12 00:00:00

abstract：:We describe a rapid in vitro assay for the evaluation of in vivo properties of conjugate vaccines. Using human and murine monoclonal antibodies (mAb) specific for lipopolysaccharides (LPS), isolated from Pseudomonas aeruginosa, we determined in a competitive binding assay the amount of LPS or conjugate vaccine which w...

journal_title：Journal of immunological methods

authors： Bruderer U,Fürer E,Cryz SJ Jr,Lang AB

更新日期：1990-10-19 00:00:00

abstract：:A simple and specific enzyme-linked immunosorbent assay (ELISA) has been developed to detect circulating IgG and IgM anti-idiotypic antibodies directed against anti-HBs molecules using 96-well polyvinyl microtitre plates as the solid phase and HRPO-labelled goat anti-HBs as conjugate. Anti-idiotype reactions were obse...

journal_title：Journal of immunological methods

authors： Irshad M,Gandhi BM,Acharya SK,Joshi YK,Tandon BN

更新日期：1987-02-11 00:00:00

abstract：:Pertussis toxin is known to elicit lymphocytosis in whooping cough patients and experimental animals, by blocking the extravasation of lymphocytes and stimulating their release from lymphoid organs such as the thymus. The mechanisms responsible for these unique effects of PT are not fully understood. The effect of per...

journal_title：Journal of immunological methods

authors： Verschueren H,Dewit J,Van der Wegen A,De Braekeleer J,Van Heule G,Dekegel D,De Baetselier P

更新日期：1991-11-22 00:00:00

abstract：:Levels of acute phase cytokines secreted ex vivo by peripheral blood mononuclear cells (PBMCs) have been shown to be associated with clinical conditions or histologic lesions in renal transplant recipients. One of the limiting factors for the potential use of this assay as a diagnostic tool is the incubation time need...

journal_title：Journal of immunological methods

authors： Mfarrej BG,Azzi J,De Serres SA

更新日期：2011-03-07 00:00:00

abstract：:We describe a method for the generation of high-affinity monoclonal antibodies, which combines the power of natural immune responses with in vitro panning, B cell culture, RT-PCR and expression of the recombinant product. B cells from immunised rabbits were incubated at approximately 1000-10,000 cells per well with so...

journal_title：Journal of immunological methods

authors： Lightwood DJ,Carrington B,Henry AJ,McKnight AJ,Crook K,Cromie K,Lawson AD

更新日期：2006-10-20 00:00:00

abstract：:We have developed a standardized procedure for the isolation of monocytes from peripheral blood by negative selection using magnetic polymer particles coated with monoclonal antibodies against T and B lymphocytes. The average purity of the monocyte suspension was 85%, and monocyte recovery was 72% from Ficoll-Hypaque ...

journal_title：Journal of immunological methods

authors： Flø RW,Naess A,Lund-Johansen F,Maehle BO,Sjursen H,Lehmann V,Solberg CO

更新日期：1991-03-01 00:00:00

abstract：:We have developed a sensitive and flexible method for the qualitative evaluation of IgG-Fc receptor interactions in cell suspensions. The assay is based on the flow cytometric quantitation of antibody-coated erythrocyte (EA) rosetting using fluorescein-labelled indicator erythrocytes (E). The number of IgG molecules o...

journal_title：Journal of immunological methods

authors： Tuijnman WB,Van de Winkel JG,Capel PJ

更新日期：1990-03-09 00:00:00

abstract：:Pathological prion protein (PrPSc) which is a conformational isoform of a host-encoded protein designated (PrPC) serves as a specific marker protein for the immunochemical diagnosis of transmissible spongiform encephalopathies (TSE). The generation of suitable antibodies to PrPSc therefore underlies the specificity an...

journal_title：Journal of immunological methods

authors： Groschup MH,Harmeyer S,Pfaff E

更新日期：1997-08-22 00:00:00

abstract：:Cut point determination is an important aspect of immunogenicity assay development. The cut point can be influenced by a myriad of factors. Key among those is the analytical variability of the assay itself and biological variation due to test samples. Since a smaller cut point value may result in improved sensitivity,...

journal_title：Journal of immunological methods

authors： Kubiak RJ,Zhang J,Ren P,Yang H,Roskos LK

更新日期：2018-12-01 00:00:00

abstract：:Goat anti-mouse antibodies conjugated with colloidal 40 nm gold particles (G40) were used as secondary layers to stain human T lymphocytes in an attempt to extend the polarized light epi-illumination microscopic technique to flow cytometric multiparameter analysis. G40-labelled T cells were further stained with phycoe...

journal_title：Journal of immunological methods

authors： Festin R,Björklund B,Tötterman TH

更新日期：1987-07-16 00:00:00

abstract：:A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OK...

journal_title：Journal of immunological methods

authors： Morizane T,Satoh I,Kumagai N,Saito H,Iino A,Inagaki Y,Watanabe T,Tsuchimoto K,Tsuchiya M

更新日期：1986-07-24 00:00:00

abstract：:Dendritic cells (DC) are increasingly explored as cellular vaccines for tumor immunotherapy. In most reported DC-based cancer vaccine trials, DC have been pulsed with soluble tumor antigen-derived peptide ligands of MHC molecules. Considering that the half-life of peptide/MHC complexes on the cell surface is relativel...

journal_title：Journal of immunological methods

authors： Waeckerle-Men Y,Scandella E,Uetz-Von Allmen E,Ludewig B,Gillessen S,Merkle HP,Gander B,Groettrup M

更新日期：2004-04-01 00:00:00

abstract：:Covalently cross-linked large and intermediate-sized IgA oligomers, prepared with IgA anti-dinitrophenyl (DNP) and bis-DNP-pimelic acid ester, were used to examine the ability of different concentrations (3.5%, 5% and 7%, w/v) of polyethylene glycol (PEG) to precipitate IgA immune complexes (IgA-IC). The size of the I...

journal_title：Journal of immunological methods

authors： Imai H,Chen A,Wyatt RJ,Rifai A

更新日期：1987-11-05 00:00:00

abstract：:The performance of immunoassays for the detection of autoantibodies is of critical importance to the diagnosis and assessment of patients with systemic lupus erythematosus (SLE). Our objective was to compare 3 multiplexed assays for measurement of multiple autoantibodies and their association with global disease activ...

journal_title：Journal of immunological methods

authors： Hanly JG,Su L,Farewell V,Fritzler MJ

更新日期：2010-06-30 00:00:00

abstract：:The T cell receptor (TCR) determines the cellular response to antigens, which are presented on the surface of target cells in the form of a peptide bound to a product of the major histocompatibility complex (pepMHC). The response of the T cell depends on the affinity of the TCR for the pepMHC, yet many TCRs have been ...

journal_title：Journal of immunological methods

authors： Chervin AS,Aggen DH,Raseman JM,Kranz DM

更新日期：2008-12-31 00:00:00

abstract：:The present study examined the use of replication-defective adenovirus for in vitro production of an immunoadhesin. A recombinant adenovirus, rendered replication defective by deletion of the E1 gene, was constructed to contain the murine interleukin-10 gene fused in frame with the hinge, CH2, and CH3 domains of the m...

journal_title：Journal of immunological methods

authors： Anglade E,Sullivan DM,Csaky KG

更新日期：1997-03-10 00:00:00

abstract：:Surface molecules exclusively expressed by cells of the bone marrow (BM) are candidate targets for delivering therapeutic agents to this tissue. To identify ligands specific for the BM, we performed a series of pannings in vivo with random peptide phage displayed libraries (RPPDL). We could show that phages bind to bo...

journal_title：Journal of immunological methods

authors： Finger AN,Bisoffi M,Wetterwald A,Gautschi E,Hohenfeld U,Klima I,Stadler BM,Mazzucchelli L,Thalmann GN,Cecchini MG

更新日期：2002-06-01 00:00:00

abstract：:The automated complement fixation system presented in this paper differs from those described by Studievic et al. (1971) and Vargues and Henley (1974). In the one developed here only one sampler is used with a wheel presenting a double row of cups. This system can assay 60 samples per h and can be modified to double t...

journal_title：Journal of immunological methods

authors： Coursaget P,Maupas P,Goudeau A,Millman I

更新日期：1976-01-01 00:00:00